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    YEAST STRAINS ENGINEERED TO PRODUCE ETHANOL FROM ACETIC ACID AND GLYCEROL
    15.
    发明公开
    YEAST STRAINS ENGINEERED TO PRODUCE ETHANOL FROM ACETIC ACID AND GLYCEROL 审中-公开
    酵母菌株生产乙酸乙酸和甘油的工艺

    公开(公告)号：EP3321368A2

    公开(公告)日：2018-05-16

    申请号：EP17180637.5

    申请日：2012-11-26

    申请人： DSM IP Assets B.V.

    发明人： TEUNISSEN, Aloysius Wilhelmus Rudolphus Hubertus ,  KLAASSEN, Paul ,  HARTMAN, Wouter Willem Antonius ,  VAN BEUSEKOM, Shimaira ,  DE BONT, Johannes Adrianus Maria

    IPC分类号： C12P7/06 ,  C12P7/08 ,  C12P7/10 ,  C12N1/18 ,  C12N1/22 ,  C12N9/02 ,  C12N9/04

    CPC分类号： C12P7/06 ,  C12N1/22 ,  C12N9/0006 ,  C12N9/0008 ,  C12P7/08 ,  C12P7/10 ,  C12Y101/01006 ,  C12Y102/01 ,  C12Y102/0101 ,  Y02E50/16 ,  Y02E50/17

    摘要： The present invention relates to processes for producing ethanol from lignocellulosic hydrolysates comprising hexoses, pentoses and acetic acid, whereby genetically modified yeast cells are use that comprise an exogenous gene encoding an acetaldehyde dehydrogenase and a bacterial gene encoding an enzyme with NAD+-linked glycerol dehydrogenase activity. The process is further characterised in that glycerol is present in or fed into the culture medium, whereby the modified yeast cell ferments the hexoses, pentoses, acetic acid and glycerol to ethanol. The invention further relates to yeast cells for use in such processes. The yeast cells advantageously comprise genetic modifications that improve glycerol utilization such as modifications that increase one or more of dihydroxyacetone kinase activity and transport of glycerol into the cell. The yeast cell further preferably comprises a functional exogenous xylose isomerase gene and/or functional exogenous genes which confer to the cell the ability to convert L-arabinose into D-xylulose 5-phosphate and they may comprise a genetic modification that increase acetyl-CoA synthetase activity.

    摘要翻译： 本发明涉及由包含己糖，戊糖和乙酸的木质纤维素水解产物生产乙醇的方法，其中使用基因修饰的酵母细胞，其包含编码乙醛脱氢酶的外源基因和编码具有NAD +连接的甘油脱氢酶活性的酶的细菌基因 。 该方法的进一步特征在于甘油存在于或加入培养基中，由此修饰的酵母细胞将己糖，戊糖，乙酸和甘油发酵成乙醇。 本发明还涉及用于这种方法的酵母细胞。 酵母细胞有利地包含改进甘油利用的遗传修饰，例如增加二羟基丙酮激酶活性中的一种或多种的修饰以及甘油转运至细胞中的修饰。 酵母细胞还优选包含赋予细胞将L-阿拉伯糖转化为D-木酮糖-5-磷酸的能力的功能性外源木糖异构酶基因和/或功能性外源基因，并且它们可包含增加乙酰-CoA合成酶 活动。

    HYDROLYSIS AND FERMENTATION PROCESS FOR ANIMAL FEED PRODUCTION
    18.
    发明授权
    HYDROLYSIS AND FERMENTATION PROCESS FOR ANIMAL FEED PRODUCTION 有权
    动物饲料生产的水解和发酵过程

    公开(公告)号：EP2696700B1

    公开(公告)日：2018-03-21

    申请号：EP12716556.1

    申请日：2012-04-13

    申请人： Ensus UK Limited

    发明人： LYWOOD, Warwick ,  PINKNEY, John ,  JAVED, Muhammad ,  EDWARDS, James

    IPC分类号： A23K10/38 ,  A23K10/12 ,  A23K20/163 ,  C12P7/08 ,  C12P7/14

    CPC分类号： A23K10/38 ,  A23K20/163 ,  C12P7/08 ,  C12P7/14 ,  Y02E50/16 ,  Y02P60/873

    摘要： A method for production of an animal feed product comprises: a) partial hydrolysis of a fermentation feedstock or the non-ethanol by-product of a fermentation process performed on a fermentation feedstock, which partial hydrolysis converts non starch polysaccharides to soluble oligomers and monomers; b) fermentation of the soluble oligomers and monomers in the partially hydrolysed feedstock or non-ethanol by-product to produce ethanol; e) recovery of the non-ethanol by-product from the fermentation of step b) to produce an animal feed product more specifically an animal feed product with improved nutritional content. A method for production of an animal feed product comprises: a) partial hydrolosis of the non ethanol by-product of a fermentation process performed on a fermentation feedstock, which partial hydrolysis converts non starch polysaccharides to soluble oligomers and monomers; b) recovery of the partially hydrolysed product from step a), to exclude the soluble oligomers and monomers, to produce an animal feed product, more specifically an animal feed product with improved nutritional content. The methods produce an animal feed product with improved nutritional content by virtue of decreased levels of pentose sugars, increased relative protein concentration, decreased relative fibre concentration, decreased levels of soluble oligomers and monomers or decreased levels of reducing sugars.

    METHOD FOR PROCESSING A BIOMASS CONTAINING LIGNOCELLULOSE
    19.
    发明公开
    METHOD FOR PROCESSING A BIOMASS CONTAINING LIGNOCELLULOSE 有权
    加工含有木质纤维素的生物质的方法

    公开(公告)号：EP3293267A1

    公开(公告)日：2018-03-14

    申请号：EP17199316.5

    申请日：2013-04-10

    申请人： Kinetic Biofuel A/S

    发明人： Bonde, Torben Andreas

    IPC分类号： C12P7/10 ,  D21C1/02 ,  A23K10/12 ,  A23K10/32 ,  C08B1/00 ,  C12P19/02 ,  C12P19/14 ,  C12P5/02

    CPC分类号： C08B1/00 ,  A23K10/12 ,  A23K10/32 ,  C12P5/023 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  C12P2203/00 ,  D21C1/02 ,  Y02E50/16 ,  Y02E50/343

    摘要： There is disclosed a method for processing a biomass (for example straw) containing lignocellulose such that cellulose and hemicellulose are made accessible for further processing, typically by decomposition, without needing energy-consuming dissolution of the biomass in water.
    The method includes repeated compressions of the biomass in a reciprocating piston press, where loose biomass is continuously fed into a piston chamber in front of a piston which moves the loose biomass into a tubular reaction chamber in which the biomass is compressed for producing a vapour explosion and autohydrolysis under simultaneous displacement of compressed biomass through the reaction chamber.
    After compression, the biomass can be added fluid livestock manure, fluid waster water sludge etc. in a biogas plant for a subsequent biogas process.

    摘要翻译： 公开了一种处理含有木质纤维素的生物质（例如稻草）的方法，使得纤维素和半纤维素通常可通过分解而进入进一步处理，而不需要能量消耗地溶解生物质在水中。 该方法包括往复式活塞压榨机中的生物质的反复压缩，其中松散的生物质被连续地供给到活塞前方的活塞室中，该活塞将松散的生物质移动到管状反应室中，其中生物质被压缩以产生蒸气爆炸 并在通过反应室同时置换压缩的生物质时自动水解。 在压缩之后，生物质可以在沼气工厂中添加流体牲畜粪便，流体废水污泥等用于随后的沼气过程。