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公开(公告)号：EP3412683B1
公开(公告)日：2022-02-09
申请号：EP18181634.9
申请日：2012-08-16
发明人： BURNETT, Luke , BOYD, Sarah Ann
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公开(公告)号：EP3908652A1
公开(公告)日：2021-11-17
申请号：EP20700136.3
申请日：2020-01-10
申请人： Fermentalg
发明人： CAGNAC, Olivier , ATHANE, Axel , DEMOL, Julien
IPC分类号： C12N1/12 , C07K14/405 , A23L33/195 , C07K1/30
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公开(公告)号：EP3334441B1
公开(公告)日：2021-06-16
申请号：EP16834772.2
申请日：2016-08-11
发明人： COOPER, Ayelet , YONAH, Nachum , BAR, Liliana
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公开(公告)号：EP2883960B1
公开(公告)日：2020-05-13
申请号：EP13828622.4
申请日：2013-08-09
申请人： Ajinomoto Co., Inc.
发明人： IWASAKI Eriko
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公开(公告)号：EP3601331A1
公开(公告)日：2020-02-05
申请号：EP18766626.8
申请日：2018-03-16
发明人： SEO, Jin Seog , MILLER, David
IPC分类号： C07K14/765 , A61K38/38 , C07K1/30 , C07K1/34 , C07K1/36
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公开(公告)号：EP3592760A2
公开(公告)日：2020-01-15
申请号：EP18716111.2
申请日：2018-03-07
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公开(公告)号：EP3521299A1
公开(公告)日：2019-08-07
申请号：EP17858396.9
申请日：2017-10-03
申请人： Spiber Inc.
发明人： HOMMA, Toshimasa
摘要： Disclosed is a method for purifying a recombinant protein of interest from a recombinant cell expressing intracellularly the recombinant protein as an insoluble matter, comprising treating the recombinant cell under conditions where a protein derived from a host cell dissolves in a first aprotic polar solvent to which an inorganic salt is added or not but the recombinant protein does not dissolve, removing a first soluble fraction, and then treating the resulting first insoluble fraction under conditions where the recombinant protein dissolves in a second aprotic polar solvent to which an inorganic salt is added.
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公开(公告)号：EP3404037A1
公开(公告)日：2018-11-21
申请号：EP17738641.4
申请日：2017-01-12
发明人： KIM, Jun Sic , KIM, Hyo Jin , PARK, Ji Yoon , LEE, Ju Ho , SON, Jae Woon , SHIN, Yong Won
摘要： The present disclosure relates to a method for purifying fibrinogen, and more particularly to a method for purifying fibrinogen, comprising the steps of: (a) precipitating fibrinogen of a fibrinogen-containing solution by adding glycine to the solution for a concentration of glycine to be 1.5 to 2.5M, and then removing a supernatant and recovering a precipitate (1 st glycine precipitation); (b) dissolving the precipitate of 1 st glycine precipitation of step (a) in a dissolution buffer to obtain a solution, precipitating the solution by adding glycine thereto for a concentration of glycine to be 0.2 to 1.2M, and recovering a supernatant (2 nd glycine precipitation); (c) precipitating the supernatant of step (b) by adding glycine thereto for a concentration of glycine to be 1.5 to 2.5M, and recovering a precipitate (3 rd glycine precipitation); and (d) dissolving the precipitate of step (c) in a dissolution buffer to obtain a solution, and subjecting the solution to nanofiltration (NF) using a nanofilter.
The novel method for purifying fibrinogen protein according to the present disclosure is a highly safe method that fundamentally blocks the entry of viruses. Furthermore, it can purify fibrinogen protein with high purity and high recovery rate by a simple process, and thus is very economical and efficient. In addition, fibrinogen purified by the method of the present disclosure has an advantage over fibrinogen purified by a conventional method in that it has higher purity, and thus has increased local activity, indicating that it can be effectively used for the prevention and treatment of blood-related diseases, particularly blood clotting diseases.-
公开(公告)号：EP2755992B1
公开(公告)日：2018-11-14
申请号：EP12832274.0
申请日：2012-09-14
IPC分类号： C07K1/30
摘要： There is a recognized need for novel, more simplified, approaches to isolation of plasma from whole blood, as well as a need to isolate cell-free plasma fractions containing different plasma proteins. Methods are divulged for use of aqueous phase systems, formed in blood or blood containing solutions via addition of a single polymer at relatively low concentration, to effect isolation (clarification) of plasma proteins from blood cells. Methods are also divulged to replace widely used Cohn-type plasma protein fractionation which is based on sequential addition of up to 40% (v/v) ethanol and other precipitants, with simple sequential addition of a polyacid. The latter results in isolation of plasma protein fractions (i.e. fibrinogen, immunoglobulin, albumin) in sequence similar to that obtained with Cohn Fractionation and therefore may be suitable for use to reduce solvent use and solvent-related process complications in existing plasma protein purification processes. It may also support use of polymeric film based containers in novel solvent free plasma fractionation processes. The methods disclosed may also be suitable for use in smaller scale plasma protein isolation, in research and diagnostic applications. The general methodologies are robust and can function over a broad range of process variables such as temperature and pH.
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公开(公告)号：EP3383889A1
公开(公告)日：2018-10-10
申请号：EP16870958.2
申请日：2016-11-23
申请人： DAEWOONG CO., LTD.
CPC分类号： C12N9/52 , B01D15/362 , B01D15/363 , B01D61/145 , B01D61/147 , B01D61/58 , B01D2315/16 , C07K14/33 , C12Y304/24069
摘要： The present invention relates to a method for producing a botulinum toxin comprising:(a) treating a culture of a botulinum toxin-producing strain with acid to form a botulinum toxin-containing precipitate; (b) adding a buffer to the botulinum toxin-containing precipitate of step (a), followed by clarification by at least one method selected from the group consisting of depth filtration (DF), microfiltration (MF), ultrafiltration (UF), sterile filtration, membrane chromatography (MC) and centrifugation; (c) subjecting the botulinum toxin-containing solution of step (b) to UF diafiltration, ammonium sulfate precipitation or hydrochloric acid precipitation, and then diluting a retentate resulting from the UF diafiltration in a buffer or dissolving a precipitate resulting from the ammonium sulfate precipitation or hydrochloric acid precipitate in a buffer; and (d) subjecting the retentate dilution, ammonium sulfate precipitate solution or hydrochloric acid precipitate solution of step (c) to anion-exchange chromatography (AEX) to purify the botulinum toxin.
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