公开(公告)号：EP4001406A3

公开(公告)日：2022-08-03

申请号：EP21205995.0

申请日：2021-11-02

Applicant: Korea Advanced Institute of Science and Technology

Inventor： LEE, Sang Yup ,  YANG, Dongsoo ,  PARK, Seon Young

IPC: C12N9/10 ,  C12P23/00 ,  C12P17/16 ,  C07K14/705 ,  C12N9/04 ,  C12N9/90 ,  C12N1/20 ,  C12N15/52

Abstract: Disclosed are a recombinant microorganism for producing a hydrophobic material, which is subjected to cell-membrane engineering in order to be imparted with at least one characteristic among an increase in a cell-membrane area, an increase in formation and secretion of an outer membrane vesicle, and an increase in formation of an inner membrane vesicle, and a cell-membrane engineering method for preparation thereof, whereby an insoluble hydrophobic material can be produced with high efficiency, the recombinant microorganism for high-efficiency production of carotenoids or violacein analogues is useful for producing natural pigments, antioxidants, antibiotics, cosmetic additives, anticancer agents, food additives, or nutritional supplements, and the natural pigment production technology developed herein achieves a great increase in production ability. Therefore, the present invention is effective at preparing a recombinant strain for efficient production of a variety of industrially and medically useful metabolites and at establishing an efficient preparation method.

公开(公告)号：EP3660157A1

公开(公告)日：2020-06-03

申请号：EP19762287.1

申请日：2019-05-02

Applicant: Korea Advanced Institute of Science and Technology

Inventor： LEE, Sang Yup ,  KIM, Kyung-Jin ,  AHN, Jung Ho ,  SEO, Hogyun ,  LEE, Jong An

IPC: C12N15/74 ,  C12N9/04 ,  C12P7/46

Abstract: Disclosed are a mutant microorganism for producing succinic acid exhibiting improved activity of conversion of oxaloacetate to malate through the introduction of genes encoding a malate dehydrogenase, wherein an amino acid residue that interacts with a pyrophosphate moiety of NADH through an amide functional group of a main chain of malate dehydrogenase is glutamine (Gln), and a method of producing succinic acid using the same. The mutant microorganism producing succinic acid according to the present invention is capable of producing a high concentration of succinic acid at the highest productivity compared to other mutant microorganisms reported to date when the microorganism is cultured in a limited medium. In addition, the mutant microorganism is capable of producing succinic acid at higher productivity and product concentration through further advanced fermentation technology.

公开(公告)号：EP3594350A1

公开(公告)日：2020-01-15

申请号：EP18760488.9

申请日：2018-02-26

Applicant: Korea Advanced Institute of Science and Technology

Inventor： LEE, Sang Yup ,  YANG, Jung Eun ,  PARK, Si Jae

IPC: C12N15/63 ,  C12N9/10 ,  C12P7/62 ,  C08G63/688

Abstract: The present invention relates to a recombinant microorganism to which a gene coding for 2-hydroxyisocaproate-CoA transferase and a gene coding for polyhydroxyalkanoate synthase are introduced and which has a potential of producing polyhydroxyalkanoate bearing an aromatic monomer or a long-chain 2-HA monomer and a method for producing polyhydroxyalkanoate bearing an aromatic monomer or a long-chain 2-HA monomer, using the recombinant microorganism. According to the present invention, a biodegradable polymer bearing an aromatic monomer or a long-chain 2-HA monomer can be produced.

公开(公告)号：EP2054502B1

公开(公告)日：2014-04-02

申请号：EP07793240.8

申请日：2007-07-25

Applicant: Korea Advanced Institute of Science and Technology

Inventor： LEE, Sang Yup ,  LIM, Sung Won ,  SONG, Hyohak

IPC: C12N1/21

CPC classification number: C12P7/46 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/1217 ,  C12N15/902 ,  C12Y203/01054 ,  Y02P20/52

公开(公告)号：EP2468884A2

公开(公告)日：2012-06-27

申请号：EP10810154.4

申请日：2010-08-18

Applicant: Korea Advanced Institute of Science and Technology ,  Avellino Co., Ltd

Inventor： LEE, Sang Yup ,  YOO, So Young ,  KIM, Do Kyun ,  PARK, Tae Jung ,  YUN, Jung Kuk ,  LEE, Gene

IPC: C12Q1/68

CPC classification number: C12Q1/6837 ,  B01J2219/00509 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00614 ,  B01J2219/00621 ,  B01J2219/00637 ,  B01J2219/00648 ,  B01J2219/00722 ,  B82Y30/00 ,  C12Q1/6883 ,  C12Q2600/158

Abstract: The present invention relates to a multi-spot metal-capped nanostructure array nucleic acid chip for diagnosing corneal dystrophy, and more particularly to a multi-spot metal-capped nanostructure array nucleic acid chip capable of employing LSPR (localized surface plasmon resonance) optical properties, a preparation method thereof, and a multi-spot metal-capped nanostructure array nucleic acid chip for diagnosing BIGH3 gene mutations, which can diagnose various corneal dystrophies. According to the invention, the metal-capped nanostructure array nucleic acid chip can be combined with analysis devices, including a light source, a detector, a spectrophotometer and a computer, to provide an LSPR optical property-based optical biosensor, and the use of the multi-spot metal-capped nanostructure array nucleic acid chip for diagnosing BIGH3 gene mutations allows the simultaneous diagnosis of various corneal dystrophies that are genetic ocular diseases.

Abstract translation: 本发明涉及一种多点金属封端的纳米结构阵列的核酸芯片用于诊断角膜营养不良，并且更具体地涉及一种多点金属封端的纳米结构阵列的核酸能够采用LSPR的芯片（局部表面等离子体共振）的光学特性 ，其制备方法，和多点金属封端的纳米结构阵列的核酸芯片用于诊断BIGH3基因突变，从而可以诊断各种角膜营养不良。 。根据本发明，金属封端的纳米结构阵列的核酸芯片可以与分析装置，包括光源，检测器，分光光度计和计算机，被组合在LSPR光学基于属性的光学生物传感器提供，并且使用的 用于诊断BIGH3基因突变的多点金属封端的纳米结构阵列的核酸芯片允许各种角膜营养不良的同时诊断是遗传的眼部疾病一样。

公开(公告)号：EP2121918B1

公开(公告)日：2011-09-28

申请号：EP07768570.9

申请日：2007-07-02

Applicant: Korea Advanced Institute of Science and Technology

Inventor： LEE, Sang Yup ,  LEE, Kwang Ho ,  PARK, Jin Hwan ,  KIM, Tae Yong, Korea Advanced Institute of Science & Technology

IPC: C12N15/09 ,  C12N1/21 ,  C12P13/08

CPC classification number: C12P13/08

Abstract: The present invention relates to a mutant microorganism producing a high concentration of L-threonine in high yield, prepared using site-specific mutation, not random mutation, such as treatment with a mutation inducer, a method for preparing the same, and a method for preparing L-threonine using the mutant microorganism producing L-threonine. By using the mutant microorganism according to the present invention, L-threonine can be prepared at high yield, additional strain development becomes possible and their physiological phenomena can be easily understood since genetic information of L-threonine producing microorganism can be identified.

公开(公告)号：EP2227541A2

公开(公告)日：2010-09-15

申请号：EP08862527.2

申请日：2008-12-18

Applicant: Korea Advanced Institute of Science and Technology

Inventor： LEE, Sang Yup ,  LEE, Jeong Wook ,  SONG, Hyohak ,  KIM, Ji Mahn ,  CHOI, Sol ,  PARK, Jin Hwan

IPC: C12N9/10 ,  C12N9/26 ,  C12N15/09 ,  C12N15/00

CPC classification number: C12N15/70 ,  C12N1/20 ,  C12N9/1205 ,  C12N9/16 ,  C12N9/2408 ,  C12P1/04 ,  C12P7/065 ,  C12P7/40 ,  C12P7/46 ,  C12P7/54 ,  C12P7/56 ,  C12P13/08 ,  C12Y207/01004 ,  C12Y207/01069 ,  C12Y302/01026 ,  Y02E50/17

Abstract: The present invention relates to a recombinant microorganism capable of metabolizing sucrose, and more particularly to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding sucrose phosphotransferase and/or a gene encoding sucrose-6-phosphate hydrolase is introduced or to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding β-fructofuranosidase is introduced. According to the present invention, a recombinant microorganism capable of using inexpensive sucrose as a carbon source instead of expensive glucose is provided. In addition, in a process of culturing microorganisms which have been incapable of using sucrose as a carbon source, sucrose can substitute for other carbon sources including glucose.

公开(公告)号：EP2121918A1

公开(公告)日：2009-11-25

申请号：EP07768570.9

申请日：2007-07-02

Applicant: Korea Advanced Institute of Science and Technology

Inventor： LEE, Sang Yup ,  LEE, Kwang Ho ,  PARK, Jin Hwan ,  KIM, Tae Yong, Korea Advanced Institute of Science & Technology

IPC: C12N15/09 ,  C12N1/21 ,  C12P13/08

CPC classification number: C12P13/08

Abstract: The present invention relates to a mutant microorganism producing a high concentration of L-threonine in high yield, prepared using site-specific mutation, not random mutation, such as treatment with a mutation inducer, a method for preparing the same, and a method for preparing L-threonine using the mutant microorganism producing L-threonine. By using the mutant microorganism according to the present invention, L-threonine can be prepared at high yield, additional strain development becomes possible and their physiological phenomena can be easily understood since genetic information of L-threonine producing microorganism can be identified.

Abstract translation: 本发明涉及使用位点特异性突变而不是随机突变（例如用突变诱导剂处理）制备高产率L-苏氨酸的突变体微生物，其制备方法以及用于制备高浓度L-苏氨酸的方法 使用产生L-苏氨酸的突变微生物制备L-苏氨酸。 通过使用根据本发明的突变微生物，可以高收率地制备L-苏氨酸，由于可以鉴定产生L-苏氨酸的微生物的遗传信息，因此可以进一步发展菌株发育并且可以容易地理解它们的生理现象。

公开(公告)号：EP1692271B1

公开(公告)日：2009-08-12

申请号：EP04734158.1

申请日：2004-05-20

Applicant: Korea Advanced Institute of Science and Technology

Inventor： LEE, Sang Yup ,  LEE, Sang Jun

IPC: C12N1/21 ,  C12P7/46 ,  C12N15/63 ,  C12N15/74 ,  C12R1/00

CPC classification number: C12P7/46 ,  C12N1/20 ,  C12R1/01

公开(公告)号：EP2054502A1

公开(公告)日：2009-05-06

申请号：EP07793240.8

申请日：2007-07-25

Applicant: Korea Advanced Institute of Science and Technology

Inventor： LEE, Sang Yup ,  LIM, Sung Won ,  SONG, Hyohak

IPC: C12N1/21

CPC classification number: C12P7/46 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/1217 ,  C12N15/902 ,  C12Y203/01054 ,  Y02P20/52

Abstract: The present invention relates to a rumen bacterial mutant producing homo- succinic acid and a method for producing homo-succinic acid using the same, and more particularly to a rumen bacterial mutant producing succinic acid at a high concentration while producing little or no other organic acids in anaerobic conditions, which is obtained by disrupting a gene encoding lactate dehydrogenase (idhA), a gene encoding phosphotransacetylase (pta), and a gene encoding acetate kinase (ackA), without disrupting a gene encoding pyruvate formate lyase (pβ), as well as a method for producing succinic acid using the same. The inventive rumen bacterial mutant has the property of having a high growth rate and succinic acid productivity while producing little or no organic acids, as compared to the prior strains producing succinic acid. Thus, the inventive rumen bacterial mutant is useful to produce succinic acid for industrial use.