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    DIRECT OLIGONUCLEOTIDE SYNTHESIS ON CDNA
    22.
    发明公开
    DIRECT OLIGONUCLEOTIDE SYNTHESIS ON CDNA 审中-公开

    公开(公告)号：EP4464778A2

    公开(公告)日：2024-11-20

    申请号：EP24203665.5

    申请日：2019-12-10

    Applicant: DNA Script

    Inventor： GODRON, Xavier ,  HORGAN, Adrian ,  GARIEL, Sylvain ,  JEDDELOH, Jeffrey ,  NICHOL, Robert ,  YBERT, Thomas

    IPC: C12N15/10

    Abstract: The invention is directed to methods for synthesizing oligonucleotides direction on biomolecules or cells living or fixed. In some embodiments, template-free enzymatic synthesis is implemented under biological conditions with successive cycles of (i) enzymatic addition of a 3'-O-blocked nucleoside triphosphate and (ii) enzymatic deblocking of the incorporated nucleotide to regenerate a free 3' hydroxyl. The invention has applications in single-cell cDNA library construction and analysis.

    HIGHLY ACTIVE CRISPR BASE EDITORS OBTAINED THROUGH CAS-ASSISTED SUBSTRATE-LINKED DIRECTED EVOLUTION (CASLIDE)
    25.
    发明公开
    HIGHLY ACTIVE CRISPR BASE EDITORS OBTAINED THROUGH CAS-ASSISTED SUBSTRATE-LINKED DIRECTED EVOLUTION (CASLIDE) 审中-公开

    公开(公告)号：EP4458963A1

    公开(公告)日：2024-11-06

    申请号：EP23171650.7

    申请日：2023-05-04

    Applicant: Technische Universität Dresden

    Inventor： SÜRÜN, Duran ,  BUCHHOLZ, Frank

    IPC: C12N9/22 ,  C12N9/78 ,  C12N15/10 ,  C12N15/90

    Abstract: The invention relates to a DNA modifying enzyme comprising or consisting of a DNA editing protein and a Cas protein and a peptide linker,
    wherein said DNA editing protein and said Cas protein are connected by said peptide linker; and
    wherein said DNA modifying enzyme has been obtained by directed molecular evolution of an original enzyme; and
    wherein said DNA modifying enzyme shows a base editing rate which is at least 10%, 20% or 30 %, preferably at least 40 %, more preferably at least 50 %, most preferably at least 60 % higher than the base editing rate of said wildtype enzyme.
    The invention relates further to a method of directed molecular evolution of a DNA modifying enzyme and to the use evolved DNA modifying enzymes in research, medicine and agriculture.

    METHODS FOR BARCODING NUCLEIC ACID MOLECULES FROM INDIVIDUAL CELLS OR CELL POPULATIONS
    26.
    发明公开
    METHODS FOR BARCODING NUCLEIC ACID MOLECULES FROM INDIVIDUAL CELLS OR CELL POPULATIONS 审中-公开

    公开(公告)号：EP4397774A3

    公开(公告)日：2024-11-06

    申请号：EP24163869.1

    申请日：2017-10-18

    Applicant: 10X Genomics, Inc.

    Inventor： MIKKELSEN, Tarjei, Sigurd ,  BELGRADER, Phillip ,  ZHENG, Xinying

    IPC: C12N15/10 ,  C12Q1/6869

    Abstract: The present disclosure provides methods, compositions and systems for analyzing individual cells or cell populations through a partitioned analysis of contents of individual cells or cell populations, such as cancer cells and cells of the immune system. Individual cells or cell populations may be co-partitioned with processing reagents for accessing cellular contents, and for uniquely identifying the content of a given cell or cell population, and subsequently analyzing the content of the cell and characterizing it as having derived from an individual cell or cell population, including analysis and characterization of nucleic acid(s) from the cell through sequencing.

    COMPOSITIONS AND METHODS FOR MODIFYING GENOMES
    28.
    发明公开
    COMPOSITIONS AND METHODS FOR MODIFYING GENOMES 审中-公开

    公开(公告)号：EP4407034A3

    公开(公告)日：2024-10-30

    申请号：EP24171775.0

    申请日：2018-08-08

    Applicant: RiceTec, Inc.

    Inventor： BEGEMANN, Matthew ,  GRAY, Benjamin N.

    IPC: C12N15/10 ,  C12N15/63 ,  C12N15/82

    Abstract: Compositions and methods for modifying genomic DNA sequences are provided. The methods produce double-stranded breaks (DSBs) at pre-determined target sites in a genomic DNA sequence, resulting in mutation, insertion, and/or deletion of DNA sequences at the target site(s) in a genome. Compositions comprise DNA constructs comprising nucleotide sequences that encode a Cms1 protein operably linked to a promoter that is operable in the cells of interest. The DNA constructs can be used to direct the modification of genomic DNA at pre-determined genomic loci. Methods to use these DNA constructs to modify genomic DNA sequences are described herein. Additionally, compositions and methods for modulating the expression of genes are provided. Compositions comprise DNA constructs comprising a promoter that is operable in the cells of interest operably linked to nucleotide sequences that encode a mutated Cms1 protein with an abolished ability to produce DSBs, optionally linked to a domain that regulates transcriptional activity. The methods can be used to up- or down-regulate the expression of genes at predetermined genomic loci.

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