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公开(公告)号：EP3266791A1
公开(公告)日：2018-01-10
申请号：EP17177650.3
申请日：2002-12-04
CPC分类号： C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C12Q1/6869 , C12Q2525/186 , C12Q2535/113 , C12Q2525/197
摘要： Nucleosides and nucleotides are disclosed that are linked to detectable labels via a cleavable linker group.
摘要翻译： 公开了核苷和核苷酸，其通过可切割的接头基团连接至可检测标记。
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32.发明授权LABELLED MODIFIED GUANINE-CONTAINING NUCLEOSIDES AND NUCLEOTIDES COMPRISING A FLUOROPHORE ATTACHED TO THE BASE THROUGH A LINKING GROUP COMPRISING A POLYETHYLENE GLYCOL SPACING GROUP AND METHODS FOR THEIR USE 有权标记的经修饰的含鸟苷环核苷和包含荧光团的核苷酸通过包含聚乙二醇间隔基团的连接基团连接到基底及其使用方法
公开(公告)号：EP1926829B2
公开(公告)日：2017-07-19
申请号：EP06779167.3
申请日：2006-08-18
发明人： LIU, Xiaohai , MILTON, John
CPC分类号： C12Q1/686 , C12Q2525/113
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公开(公告)号：EP3177634A1
公开(公告)日：2017-06-14
申请号：EP15750100.8
申请日：2015-08-06
发明人： WU, Xiaolin , LIU, Xiaohai
IPC分类号： C07H19/14
摘要： Some embodiments of the present application relate to novel modified nucleotide linkers for increasing the efficiency of nucleotide incorporation in Sequencing by Synthesis applications. Methods of preparing these modified nucleotide linkers are also provided herewith.
摘要翻译： 本申请的一些实施方案涉及用于增加合成应用测序中的核苷酸掺入效率的新型修饰的核苷酸接头。 本文还提供了制备这些修饰的核苷酸接头的方法。
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34.发明授权LABELLED MODIFIED GUANINE-CONTAINING NUCLEOSIDES AND NUCLEOTIDES COMPRISING A FLUOROPHORE ATTACHED TO THE BASE THROUGH A LINKING GROUP COMPRISING A POLYETHYLENE GLYCOL SPACING GROUP AND METHODS FOR THEIR USE 有权标为已修改鸟嘌呤核苷含核苷酸，并用共价结合荧光团在该基地，其中左统一COMPONENT聚乙二醇单位遏制和应用它们的方法
公开(公告)号：EP1926829B1
公开(公告)日：2014-08-06
申请号：EP06779167.3
申请日：2006-08-18
发明人： LIU, Xiaohai , MILTON, John
CPC分类号： C12Q1/686 , C12Q2525/113
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公开(公告)号：EP3623481A1
公开(公告)日：2020-03-18
申请号：EP19189927.7
申请日：2012-09-21
申请人： Illumina, Inc.
发明人： BOUTELL, Jonathan Mark , ELTOUKHY, Helmy A. , FENG, Wenyi , HIRSCHBEIN, Bernard , JOSEPH, Thomas , KAIN, Robert C. , KLAUSING, Kay , LIU, Xiaohai , SHEN, Min-Jui Richard , SMITH, Geoffrey Paul , SMITH, Randall , TREGIDGO, Carolyn , WU, Xiaolin
IPC分类号： C12Q1/68
摘要： The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.
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公开(公告)号：EP4388127A1
公开(公告)日：2024-06-26
申请号：EP22793322.3
申请日：2022-08-16
申请人： Illumina, Inc.
IPC分类号： C12Q1/6806 , C12Q1/6869 , C07C245/00
CPC分类号： C12Q1/6806 , C12Q1/6869
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公开(公告)号：EP4347885A1
公开(公告)日：2024-04-10
申请号：EP22731433.3
申请日：2022-05-26
申请人： ILLUMINA, INC.
IPC分类号： C12Q1/6874 , G01N21/64
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公开(公告)号：EP3290528A1
公开(公告)日：2018-03-07
申请号：EP17180583.1
申请日：2012-09-21
申请人： Illumina, Inc.
发明人： BOUTELL, Jonathan Mark , ELTOUKHY, Helmy A. , FENG, Wenyl , HIRSCHBEIN, Bernard , JOSEPH, Thomas , KAIN, Robert C. , KLAUSING, Kay , LIU, Xiaohai , SHEN, Min-Jui Richard , SMITH, Geoffrey Paul , SMITH, Randall , TREGIDGO, Carolyn , WU, Xiaolin
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6869 , C12Q2525/113 , C12Q2535/122 , C12Q2563/107
摘要： The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.
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公开(公告)号：EP3981886A1
公开(公告)日：2022-04-13
申请号：EP21189843.2
申请日：2012-09-21
申请人： Illumina, Inc.
发明人： BOUTELL, Jonathan Mark , ELTOUKHY, Helmy A. , FENG, Wenyi , HIRSCHBEIN, Bernard , JOSEPH, Thomas , KAIN, Robert C. , KLAUSING, Kay , LIU, Xiaohai , SHEN, Min-Jui Richard , SMITH, Geoffrey Paul , TREGIDGO, Carolyn , WU, Xiaolin , SMITH, Randall
IPC分类号： C12Q1/6869 , C12Q1/6874
摘要： The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected. Disclosed is also a reversibly blocked nucleotide conjugate, which is indirectly labelled with a hapten attached via a linker to the nucleobase, wherein the hapten is detectable by a binding partner-fluorescent moiety conjugate.
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40.发明公开CONTINUOUS EXTENSION AND DEBLOCKING IN REACTIONS FOR NUCLEIC ACID SYNTHESIS AND SEQUENCING 审中-公开核酸合成和测序反应中的连续扩展和解旋
公开(公告)号：EP3293272A1
公开(公告)日：2018-03-14
申请号：EP17180245.7
申请日：2012-09-28
申请人： Illumina, Inc.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q2521/101 , C12Q2523/107 , C12Q2525/101 , C12Q2525/186 , C12Q2565/1015
摘要： A reaction mixture including (a) a nucleic acid having a primer hybridized to a template, (b) nucleotide analogs, wherein the nucleotide analogs have a blocking moiety; (c) a polymerase that is capable of forming an extended primer by adding the nucleotide analogs to the primer, and (d) a deblocking agent that is capable of removing the blocking moiety from the extended primer. Also provided is a method of synthesizing a polynucleotide including sequentially adding a plurality of the different nucleotides analogs to the nucleic acid via several reaction cycles in the reaction mixture, wherein each reaction cycle includes (i) the polymerase adding a nucleotide analog to the nucleic acid to form a transient nucleic acid species comprising a blocking moiety, and (ii) the deblocking agent modifying the transient nucleic acid species to remove the blocking moiety.
摘要翻译： （a）具有与模板杂交的引物的核酸，（b）核苷酸类似物，其中所述核苷酸类似物具有封闭部分的反应混合物; （c）能够通过将核苷酸类似物添加至引物而形成延伸引物的聚合酶，和（d）能够从延伸引物中去除封闭部分的去封闭剂。 还提供了合成多核苷酸的方法，包括在反应混合物中通过几个反应循环将多个不同的核苷酸类似物依次添加至核酸，其中每个反应循环包括（i）聚合酶将核苷酸类似物添加至核酸 以形成包含阻断部分的瞬时核酸种类，和（ii）去封闭剂修饰瞬时核酸种类以除去阻断部分。
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