NOVEL CELLULASE GENE
    34.
    发明公开
    NOVEL CELLULASE GENE 有权
    中毒剂细胞因子

    公开(公告)号:EP2554667A1

    公开(公告)日:2013-02-06

    申请号:EP10848946.9

    申请日:2010-03-31

    摘要: An object is to identify endoglucanase and β-glucosidase genes by isolating genomic DNA containing cellulase genes, which are classified into endoglucanases or β-glucosidases, from Acremonium cellulolyticus , and sequencing the nucleotide sequences thereof. The inventors intensively compared the amino acid sequences of known endoglucanases and β-glucosidases with each other to find conserved region of amino acid sequences in Acremonium cellulolyticus , and various primers were designed based on the information. PCR was carried out using the various primers thus designed and genomic DNA or cDNA as a template. As a result, gene fragments of endoglucanases and β-glucosidases were obtained. Primers were designed based on the gene fragments, and PCR was carried out to amplify nine genes of endoglucanases and β-glucosidases. The nucleotide sequences thereof were sequenced, and the present invention was completed.

    摘要翻译: 目的是通过分离含有纤维素分解的内切葡聚糖酶或β-葡糖苷酶的含有纤维素酶基因的基因组DNA,并对其核苷酸序列进行测序来鉴定内切葡聚糖酶和β-葡糖苷酶基因。 本发明人将已知内切葡聚糖酶和β-葡糖苷酶的氨基酸序列彼此进行了深入比较,以发现解纤维解淀粉中的氨基酸序列的保守区域,并根据该信息设计了各种引物。 使用如此设计的各种引物进行PCR,并将基因组DNA或cDNA作为模板进行。 结果得到内切葡聚糖酶和β-葡糖苷酶的基因片段。 基于基因片段设计引物,进行PCR扩增内切葡聚糖酶和β-葡糖苷酶的9个基因。 对其核苷酸序列进行测序,完成本发明。

    PAPER COATING OR BINDING FORMULATIONS AND METHODS OF MAKING AND USING SAME
    35.
    发明公开
    PAPER COATING OR BINDING FORMULATIONS AND METHODS OF MAKING AND USING SAME 审中-公开
    纸张涂布纸或粘合制剂和方法生产及其用途

    公开(公告)号:EP2550396A1

    公开(公告)日:2013-01-30

    申请号:EP11710467.9

    申请日:2011-03-21

    申请人: BASF SE

    发明人: HAYES, Peter C.

    IPC分类号: D21H19/56 D21H21/10

    CPC分类号: D21H19/56 D21H21/10

    摘要: Paper coating or binding formulations are provided that comprise aqueous copolymer emulsions including copolymers derived from one or more copolymerizable surfactants of formula I and one or more monomers. The paper coating or binding formulations can include one or more mineral fillers, coating pigments, or mixtures thereof, wherein the total weight concentration of the one or more mineral fillers, coating pigments, or mixtures thereof is 65% or higher, based on the total weight of the composition. Paper products coated with the paper coating or binding formulations, methods of making the paper coating or binding formulations, and methods of making the coated paper products are also disclosed.