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1.发明公开METHOD FOR PRODUCING ARMORACIA RUSTICANA-DERIVED PEROXIDASE RECOMBINANT PROTEIN USING FILAMENTOUS FUNGI 有权用于生产辣根来源的重组PEROXIDASEPROTEINEN使用线程蘑菇
公开(公告)号：EP2857511A4
公开(公告)日：2016-07-20
申请号：EP13798125
申请日：2013-05-30
发明人： YOKOYAMA FUMIKAZU , OKAKURA KAORU , INOUE ATSUSHI , MURASHIMA KOICHIRO , NAGASATO TOSHIAKI , YANAI KOJI , NAKANE AKITAKA
CPC分类号： C12N9/0065 , C12Q1/28 , C12Y111/01007 , D06L4/40 , G01N33/535 , G01N33/581
摘要： A modified polynucleotide has a different base sequence in at least one codon from a wild-type base sequence encoding a horseradish peroxidase polypeptide. The usage frequency of the modified codon of the polynucleotide corresponds to the codon usage frequencies of three filamentous fungal species in Humicola, Aspergillus, and Trichoderma. The polynucleotide is capable of expressing the polypeptide to be encoded in a filamentous fungus.
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2.发明公开MUTANT XYLANASE, MANUFACTURING METHOD AND USE THEREFOR, AND METHOD FOR MANUFACTURING SACCHARIFIED LIGNOCELLULOSE 有权突变体木聚糖酶，工艺用于生产SACCHARIFIZIERTER木质纤维素PRODUCING及其用途和方法
公开(公告)号：EP2784161A4
公开(公告)日：2015-11-25
申请号：EP12851111
申请日：2012-11-22
发明人： YANAI HISAAKI , TAMAI HIROKI , OSABE MASAMI , YOKOYAMA FUMIKAZU , OKAKURA KAORU , INOUE ATSUSHI
IPC分类号： C12N15/56 , A21D8/04 , A23K1/165 , C11D3/386 , C12N1/15 , C12N1/19 , C12N1/21 , C12N9/24 , C12P19/02 , C12P19/14 , C13K1/02 , C13K13/00 , D06L4/40 , D21C5/00
CPC分类号： C12N9/2482 , A21D8/042 , A23K20/189 , C11D3/38636 , C12N9/248 , C12P19/02 , C12P19/14 , C12Y302/01004 , C12Y302/01008 , C12Y302/01021 , C13K1/02 , C13K13/002 , Y02E50/16 , Y02P20/52
摘要： What is aimed at is provision of an inexpensive and efficient saccharification method for lignocellulose using a thermostable xylanase and provision of a mutant xylanase that has a substitute amino acid residue, and that exhibits stable activity even under severe conditions in which enzymes easily inactivate, and that provides an initial rate of reaction not significantly reduced as compared to a wild-type xylanase corresponding to the mutant xylanase. Provided is a method of producing a saccharified product of lignocellulose, including contacting a lignocellulosic raw material with a thermostable xylanase, and a mutant xylanase that provides an initial rate of reaction that is at least 70% of that provided by a wild-type xylanase corresponding thereto, that has a xylanase activity after heat treatment at 50 °C for 24 hours that is at least 50% of its xylanase activity before the heat treatment, and that has a substitute amino acid residue.
摘要翻译： 什么在旨在是使用突变体木聚糖酶的一个热电表木聚糖酶和佣金规定木质纤维素的廉价和有效的糖化方法的做有一个取代氨基酸残基，甚至是在其中酶容易失活的苛刻条件没有表现出稳定的活性，并没有 提供在不显着降低相对于野生型木聚糖酶对应于突变体木聚糖酶初始反应速率。 提供的是生产木质纤维素的糖化产品，包括接触木质纤维素原料与热塑性表木聚糖酶的方法，和突变的木聚糖酶没有提供在没有反应的初始速率并通过野生型木聚糖酶提供了至少70％CORRESPONDING 于此，确实具有在50℃下热处理后的木聚糖酶活性24小时所做的是其木聚糖酶活性的至少50％的热处理前，也确实具有取代氨基酸残基。
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公开(公告)号：EP2468859A4
公开(公告)日：2013-03-13
申请号：EP10809957
申请日：2010-08-17
发明人： YOKOYAMA FUMIKAZU , YOKOYAMA KENGO , MAZUKA NOBUKO
IPC分类号： C12N15/09 , A23K1/16 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/42 , C12P19/14 , C12P21/02 , D21C5/02 , D21H17/22
CPC分类号： C12N9/2445 , A23K10/14 , A23K10/32 , C12P19/14 , C12Y302/01021 , D06M15/15 , D21C5/005 , D21H17/005 , D21H17/22 , D21H21/10
摘要： By combination of hydrophobic chromatography and strongly basic anion-exchange chromatography, a novel, highly hydrophobic ²-glucosidase was successfully identified from Acremonium cellulolyticus. Further, a gene corresponding to the identified ²-glucosidase was isolated. When multiple modifications were introduced into the base sequence of the gene, the gene was successfully expressed in Trichoderma viride at a high level, and the expression product successfully exhibited a high ²-glucosidase activity.
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公开(公告)号：EP2554667A4
公开(公告)日：2013-09-04
申请号：EP10848946
申请日：2010-03-31
发明人： YOKOYAMA FUMIKAZU
CPC分类号： C12N9/2437 , A23K10/14 , A23K20/189 , C12N9/2445 , C12P19/02 , C12P19/14 , C12P2203/00 , C12Y302/01004 , C12Y302/01021 , D06M16/003 , D21C5/005 , D21C5/025 , D21H21/10 , Y02E50/16 , Y02W30/648
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