公开(公告)号：EP1152054B1

公开(公告)日：2005-03-23

申请号：EP00977938.0

申请日：2000-11-24

申请人： Kaneka Corporation

发明人： KIZAKI, Noriyuki ,  YAMADA, Yukio ,  YASOHARA, Yoshihiko ,  HASEGAWA, Junzo

IPC分类号： C12N9/02 ,  C12N15/53 ,  C12N9/04 ,  C12N1/21 ,  C12P7/62

CPC分类号： C12N9/0006 ,  C12P7/62 ,  C12P41/00 ,  C12P41/002

摘要： A novel polypeptide capable of forming tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate; and a polynucleotide encoding the same. Namely, a polypeptide which has an enzymatic activity of assymetrically reducing tert-butyl (S)-6-chloro-5-hydroxy-3-oxohexanoate to give tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate and contains the amino acid sequence represented by SEQ ID NO:1 in Sequence Listing or an amino acid sequence derived from the above amino acid sequence by the substitution, insertion, deletion or addition of at lease one amino acid.

公开(公告)号：EP0916654B1

公开(公告)日：2005-02-23

申请号：EP97933892.8

申请日：1997-08-01

申请人： KANEKA CORPORATION

发明人： YASOHARA, Yoshihiko ,  MIYAMOTO, Kenji ,  KAWANO, Shigeru ,  HASEGAWA, Junzo

IPC分类号： C07C309/64 ,  C07C309/72 ,  C07C303/30 ,  C07B53/00

CPC分类号： C07C303/30 ,  C07B2200/07 ,  C07C309/64 ,  C07C309/66 ,  C07C309/73 ,  C07C327/32 ,  C07D317/24

公开(公告)号：EP1055732A1

公开(公告)日：2000-11-29

申请号：EP99959901.2

申请日：1999-12-20

申请人： KANEKA CORPORATION

发明人： TAOKA, Naoaki ,  KIZAKI, Noriyuki ,  YASOHARA, Yoshihiko ,  INOUE, Kenji ,  HASEGAWA, Junzo

IPC分类号： C12P7/22

CPC分类号： C12P7/22 ,  C12P41/002

摘要： The present invention is related to producing (R)-2-hydroxy-1-phenoxypropane derivatives, which are of value as intermediates of pharmaceuticals, with high optical purity and good efficiency,

comprising a step of reducing a phenoxyacetone derivative with a carbonyl reductase derived from a microorganism of the genus Candida or a culture broth or processed culture broth of said microorganism having the ability to elaborate the carbonyl reductase.

摘要翻译： 本发明涉及生产具有高光学纯度和高效率的作为药物中间体的（R）-2-羟基-1-苯氧基丙烷衍生物，该方法包括用衍生的羰基还原酶还原苯氧基丙酮衍生物的步骤 来自假丝酵母属的微生物或培养肉汤或所述微生物的加工过的培养肉汤，其能够精制所述羰基还原酶。

公开(公告)号：EP1024139A1

公开(公告)日：2000-08-02

申请号：EP99935066.3

申请日：1999-08-05

申请人： KANEKA CORPORATION

发明人： KIZAKI, Noriyuki ,  YAMADA, Yukio ,  YASOHARA, Yoshihiko ,  NISHIYAMA, Akira ,  MIYAZAKI, Makoto ,  MITSUDA, Masaru ,  KONDO, Takeshi ,  UEYAMA, Noboru ,  INOUE, Kenji

IPC分类号： C07D319/06 ,  C07D319/08 ,  C07C59/90 ,  C07C51/353 ,  C07C59/115 ,  C12P7/04

CPC分类号： C07D319/06 ,  C07B2200/07 ,  C07C67/343 ,  C07C253/30 ,  C12P7/62 ,  C07C69/716 ,  C07C255/21

摘要： This invention provides a process for producing optically active 2-[6-(hydroxymethyl)-1,3-dioxan-4-yl] acetic acid derivatives, which are of value as intermediates of drugs, from inexpensive starting materials without using any special equipment such as that required for super-low temperature reactions.
A process for producing optically active 2-[6-(hydroxymethyl)-1,3-dioxan-4-yl]acetic acid derivatives which comprises

reacting an acetic acid derivative at a temperature of not less than -30°C with an enolate prepared by permitting either a base or a metal having a valency of 0 to act on the derivative to produce a hydroxyoxohexanoic acid derivative,
reducing this compound with the aid of a strain of microorganism to provide a halomethyldioxanylacetic acid derivative,
treating this compound with an acetalizing agent in the presence of an acid catalyst to provide a halomethyldioxanylacetic acid derivative,
reacted with an acyloxylating agent to provide a acyloxymethyldioxanylacetic acid derivative, and
subjecting this compound to solvolysis in the presence of a base.

摘要翻译： 本发明提供了一种从廉价起始原料生产具有价值作为药物中间体的光学活性2-δ-（羟甲基）-1,3-二恶烷-4-基乙酸衍生物的方法，而不使用任何特殊设备，例如 这是超低温反应所需的。 一种制备光学活性的2-δ-（羟甲基）-1,3-二恶烷-4-基乙酸衍生物的方法，其包括使不饱和-30℃的乙酸衍生物与通过使任一种 碱或金属，其化合价为0，作用于衍生物以产生羟基己酸衍生物，借助微生物菌株还原该化合物，得到卤代甲基二恶烷基乙酸衍生物，在缩合剂存在下用缩醛化剂处理该化合物 的酸催化剂，得到卤代甲基二恶烷基乙酸衍生物，与酰氧基化剂反应，得到酰氧基甲基二恶烷基乙酸衍生物，并在碱的存在下使其溶解。

公开(公告)号：EP0967271A1

公开(公告)日：1999-12-29

申请号：EP97937861.9

申请日：1997-09-01

申请人： KANEKA CORPORATION

发明人： YASOHARA, Yoshihiko ,  KIZAKI, Noriyuki ,  HASEGAWA, Junzo ,  WADA, Masaru, Usuki-manshon 305 ,  SHIMIZU, Sakayu, 6-9, Tokiwayamashita-cho ,  KATAOKA, Michihiko, Haitsukosumo 227 ,  YAMAMOTO,K. Nihontabaccosangyo Kab. Hiyoshidai-ryo ,  KAWABATA, Hiroshi, Aburesuto Iwakura 1204 ,  KITA, Keiko, Godoshukusha Kosanjyutaku 3-105

IPC分类号： C12N9/02 ,  C12N15/53 ,  C12N15/63 ,  C12N1/21 ,  C12P7/62

CPC分类号： C12P7/62 ,  C12N9/0006 ,  C12P41/002

摘要： An enzyme having carbonyl reduction activity of reducing a carbonyl compound asymmetrically to produce an optically active alcohol, a DNA coding the enzyme, a plasmid having the DNA, a transformant which is a cell transformed with the plasmid, and a production method of an optically active alcohol using the enzyme and/or the transformed cell are provided.

摘要翻译： 还原羰基化合物的具有酶活羰基还原活性不对称产生对光学活性醇，DNA编码的酶，具有DNA，转化体的所有其是用该质粒转化的细胞，和光学活性的制造方法的质粒 同时提供了使用酶和/或转化的细胞醇。

公开(公告)号：EP0916654A1

公开(公告)日：1999-05-19

申请号：EP97933892.8

申请日：1997-08-01

申请人： KANEKA CORPORATION

发明人： YASOHARA, Yoshihiko ,  MIYAMOTO, Kenji ,  KAWANO, Shigeru ,  HASEGAWA, Junzo

IPC分类号： C07C309/64 ,  C07C309/72 ,  C07C303/30 ,  C07B53/00

CPC分类号： C07C303/30 ,  C07B2200/07 ,  C07C309/64 ,  C07C309/66 ,  C07C309/73 ,  C07C327/32 ,  C07D317/24

摘要： Novel and useful optical active 2-aralkyl-3-sulfonyloxy-1-propanol and 2-aralkyl-3-sulfonyloxypropionic acid are provided by using an optical active 2-aralkyl-3-acyloxy-1-propanol as a starting material. Furthermore, an optical active 2-aralkyl-3-thiopropionic acid, which is an important intermediate of enkephalinase inhibitor, is provided. According to the present invention, industrially useful optical active sulfonic acid ester derivatives can be provided.

摘要翻译： 通过使用光学活性2-芳烷基-3-酰氧基-1-丙醇作为起始原料提供了新的和有用的光学活性2-芳烷基-3-磺酰氧基-1-丙醇和2-芳烷基-3-磺酰氧基丙酸。 此外，提供了作为脑啡肽酶抑制剂的重要中间体的光学活性2-芳烷基-3-硫代丙酸。 根据本发明，可以提供工业上有用的光学活性磺酸酯衍生物。