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    METHODS AND APPARATUSES FOR CHIP-BASED DNA ERROR REDUCTION
    42.
    发明公开
    METHODS AND APPARATUSES FOR CHIP-BASED DNA ERROR REDUCTION 有权
    方法和设备的基于芯片的DNA减少错误

    公开(公告)号：EP2504449A1

    公开(公告)日：2012-10-03

    申请号：EP10784391.4

    申请日：2010-11-19

    申请人： Gen9, Inc.

    发明人： RAMU, Senthil ,  JACOBSON, Joseph ,  CHU, Larry Li-Yang

    IPC分类号： C12Q1/68

    CPC分类号： C12N15/1058 ,  C12Q1/6848 ,  C12Q2521/514 ,  C12Q2565/537

    摘要： Methods and apparatus relate to reduction of sequence errors generated during synthesis of nucleic acids on a microarray chip. The error reduction can include synthesis of complementary stands (to template strands), using a short universal primer complementary to the template strands and polymerase. Heteroduplex can be formed be melting and re-annealing complementary stands and template strands. The heteroduplexes containing a mismatch can be recognized and cleaved by a mismatch endonuclease. The mismatch-containing cleaved heteroduplexes can be removed from the microarray chip using a global buffer exchange. The error free synthetic nucleic acids generated therefrom can be used for a variety of applications, including synthesis of biofuels and value-added pharmaceutical products.

    Microarray synthesis and assembly of gene-length polynucleotides
    43.
    发明公开
    Microarray synthesis and assembly of gene-length polynucleotides 有权
    MIKROARRAY-SYNTHESE UND -ZUSAMMENBAU VONGENLÄNGEAUFWEISENDEN POLYNUKLEOTIDEN

    公开(公告)号：EP2330216A1

    公开(公告)日：2011-06-08

    申请号：EP10013056.6

    申请日：2003-09-12

    申请人： Gen9, Inc.

    发明人： Oleinikov, Andrew V.

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12N15/1068 ,  B01J19/0046 ,  B01J2219/00378 ,  B01J2219/00385 ,  B01J2219/00432 ,  B01J2219/00454 ,  B01J2219/005 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00626 ,  B01J2219/00639 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00677 ,  B01J2219/00689 ,  B01J2219/00713 ,  B01J2219/00722 ,  B82Y30/00 ,  C12Q1/6837 ,  C40B40/06 ,  C40B50/14 ,  C40B80/00

    摘要： The invention relates to a process for assembling a polynucleotide from a plurality of oligonucleotides comprising: (a) synthesizing or spotting a plurality of different oligonucleotides on a microarray device or bead device having a solid or porous surface, wherein the plurality of oligonucleotides are attached to the solid or porous surface, and wherein each oligonucleotide comprises a sequence region of from about 10 to about 50 bases that is the same or substantially the same as a sequence region of another oligonucleotide; (b) forming an oligonucleotide complementary to a first oligonucleotide by extending a first primer, wherein said primer is complementary to a portion of said first oligonucleotide; (c) is associating said complementary oligonucleotide from said first oligonucleotide and annealing said complementary oligonucleotide to another oligonucleotide comprising a sequence region that is the same or substantially the same as a sequence region of said first oligonucleotide; (d) repeating the primer extension cycles of step (c) to produce a full-length complementary polynucleotide; and (e) amplifying the assembled full length complementary polynucleotide to produce a full length polynucleotide in desired quantities.

    摘要翻译： 本发明涉及一种从多个寡核苷酸组装多核苷酸的方法，包括：（a）在具有固体或多孔表面的微阵列装置或珠粒装置上合成或点样多种不同寡核苷酸，其中多个寡核苷酸附着于 固体或多孔表面，并且其中每个寡核苷酸包含与另一寡核苷酸的序列区相同或基本相同的约10至约50个碱基的序列区域; （b）通过延伸第一引物形成与第一寡核苷酸互补的寡核苷酸，其中所述引物与所述第一寡核苷酸的一部分互补; （c）将所述互补寡核苷酸与所述第一寡核苷酸缔合，并将所述互补寡核苷酸退火至包含与所述第一寡核苷酸的序列区相同或基本相同的序列区的另一寡核苷酸; （d）重复步骤（c）的引物延伸循环以产生全长互补多核苷酸; 和（e）扩增组装的全长互补多核苷酸以产生所需量的全长多核苷酸。

    METHODS FOR NUCLEIC ACID ASSEMBLY AND HIGH THROUGHPUT SEQUENCING
    49.
    发明授权
    METHODS FOR NUCLEIC ACID ASSEMBLY AND HIGH THROUGHPUT SEQUENCING 有权

    公开(公告)号：EP2864531B1

    公开(公告)日：2018-10-24

    申请号：EP13809969.2

    申请日：2013-06-24

    申请人： Gen9, Inc.

    发明人： HUDSON, Michael, E. ,  KUNG, Li-yun A. ,  SCHINDLER, Daniel ,  ARCHER, Stephen ,  SAAEM, Ishtiaq

    IPC分类号： C40B50/06 ,  C12N15/10 ,  C12N15/66

    CPC分类号： C12N15/1031 ,  C12N15/1093 ,  C12N15/66 ,  C12Q2521/301 ,  C12Q2521/501

    摘要： Methods and apparatus of some aspects of the invention relate to the synthesis of high fidelity polynucleotides. In particular, aspects of the invention relate to concurrent enzymatic removal of amplification sequences and ligation of processed oligonucleotides into nucleic acid assemblies. According to some embodiments, the invention provides a method for producing a target nucleic acid having a predefined sequence. In some embodiments, the method comprises the step of providing a plurality of oligonucleotides, wherein each oligonucleotides comprises (i) an internal sequence identical to a different portion of a sequence of a target nucleic acid, (ii) a 5′ sequence flanking the 5′ end of the internal sequence and a 3′ flanking sequence flanking the 3′ end of the internal sequence, each of the flanking sequence comprising a primer recognition site for a primer pair and a restriction enzyme recognition site.