公开(公告)号：EP0202300A1

公开(公告)日：1986-11-26

申请号：EP85906001.0

申请日：1985-11-18

申请人： Schering Biotech Corporation

发明人： YOKOTA, Takashi ,  LEE, Frank, Don ,  RENNICK, Donna, Maye ,  ARAI, Ken-ichi

IPC分类号： C12N15 ,  A61K38 ,  A61P37 ,  C07H21 ,  C07K14 ,  C12N1 ,  C12N5 ,  C12P21 ,  C12R1

CPC分类号： C07K14/535 ,  A61K38/00

摘要： Vecteurs plasmidiques véhiculant des clones d'ADN complémentaire (cADN) codant les polypeptides présentant une activité de facteur de stimulation de colonies de granulocytes/macrophages humains, comprenant une activité de facteur de croissance d'éosinophiles. L'un de ces polypeptides comporte en longueur environ 144 acides aminés, y compris une séquence d'amorce potentielle. Le cADN est dérivé de l'ARN messager isolé d'une source cellulaire humaine après activation avec de la concavaline A. Le cADN a été cloné par incorporation dans un vector plasmidique, transformé par la suite en E. coli. Le vecteur plasmidique contenait également des segments d'ADN provenant du virus SV40, permettant l'expression du cADN après transfection dans une cellule hôte de mammifère, telle que des cellules COS-7 de singes.

公开(公告)号：EP0177357A1

公开(公告)日：1986-04-09

申请号：EP85307094.4

申请日：1985-10-03

申请人： Schering Biotech Corporation

发明人： Lee, Frank Don ,  Yokota, Takashi ,  Arai, Ken-ichi

IPC分类号： C12N15/26 ,  C12P21/02 ,  C07K13/00 ,  C12P19/38 ,  C12N1/20 ,  C12N5/00 ,  C12Q1/00

CPC分类号： C12N15/81 ,  C07K14/55 ,  C12N15/1096

摘要： Plasmid vectors are provided that carry complementary DNA (cDNA) clones coding for polypeptides exhibiting murine interieukin-2 activity. One of these polypeptides is 168 amino acids in length, including a potential leader sequence of about 19 amino acids. The cDNA is derived from messenger RNA isolated from a mouse T-cell line after activation with concanavalin A. The cDNA was cloned by incorporation into a plasmid vector, which is then transformed into E. coli. The plasmid vector also contained DNA segments from the SV40 virus, permitting expression of the cDNA after transfection into a mammalian host cell, such as monkey COS-7 cells.

摘要翻译： 提供携带编码表达鼠白介素-2活性的多肽的互补DNA（cDNA）克隆的质粒载体。 这些多肽之一是长度为168个氨基酸，包括约19个氨基酸的潜在前导序列。 cDNA是用刀豆球蛋白A活化后从小鼠T细胞系分离的信使RNA衍生的。通过掺入到质粒载体中克隆cDNA，然后转化到大肠杆菌中。 质粒载体还含有来自SV40病毒的DNA片段，其在转染到哺乳动物宿主细胞如猴COS-7细胞后能够表达cDNA。

公开(公告)号：EP0155192A2

公开(公告)日：1985-09-18

申请号：EP85301834.9

申请日：1985-03-15

申请人： Schering Biotech Corporation ,  THE JOHNS HOPKINS UNIVERSITY

发明人： Martens, Christine L. ,  Ishizaka, Kimishige ,  Moore, Kevin W. ,  Huff, Thomas F.

IPC分类号： C12P21/02 ,  C12N15/12 ,  A61K37/02

CPC分类号： C07K14/47 ,  A61K38/00 ,  Y10S930/26

摘要： Plasmid vectors are provided that carry complementary DNA (cDNA) clones coding for polypeptides exhibiting mammalian IgE binding factor activity. One of these clones contains an open reading frame consisting of 556 codons. The cDNA is derived for example from messenger RNA isolated from a rat/mouse T-cell hybridoma line. The cDNA can be cloned by incorporation into a pcD plasmid vector. The plasmid vector also contains DNA segments from th SV40 virus, permitting expression of the cDNA to form a polypeptide having IgE potentiating activity after transfection into a mammalian host cell, such as monkey Cos7 cells.

公开(公告)号：EP0138133A1

公开(公告)日：1985-04-24

申请号：EP84111677.5

申请日：1984-09-29

申请人： Schering Biotech Corporation

发明人： Yokota, Takashi ,  Lee, Frank Don ,  Rennick, Donna Maye ,  Arai, Ken-ichi

IPC分类号： C12N15/27 ,  C07K15/06 ,  C07H21/04 ,  C12P21/00 ,  A61K37/02

CPC分类号： C07K14/52 ,  A61K38/00 ,  Y10S930/12 ,  Y10S930/14 ,  Y10S930/141 ,  Y10S930/30

摘要： Plasmid vectors are provided that carry complementary DNA (cDNA) clones coding for polypeptides exhibiting mammalian multi-lineage cellular growth activity and/or mammalian mast cell growth factor activity. One of these polypeptides is 166 amino acids in length, including a potential leader sequence of about 19 amino acids. The cDNA is derived from messenger RNA isolated from a mouse T-cell line after activation with concanavalin A. The cDNA was cloned by incorporation into a plasmid vector, which then transformed into E. coli. The plasmid vector also contained DNA segments from the SV40 virus, permitting expression of the cDNA after transfection into a mammalian host cell, such as monkey COS-7 cells.