摘要:
Provided is a method which is capable of determining, at an initial stage of differentiation, the differentiated state of undifferentiated stem cells. The present invention relates to "a method for determining a differentiated state of a cell comprising detecting expression of FOXB2 gene of a stem cell, and determining the differentiated state based on the result", and "a differentiation marker selected from mRNA or protein derived from the FOXB2 gene". The present invention is applicable to quality management of the stem cells or to methods for preparation and isolation of the differentiated cells. Further, because the differentiated cells can be determined at an initial stage of culture, the present invention is useful for an early stage screening of cells and for quality management of the stem cells, and reduction in culturing period and cost reduction in expenditure regarding a culture medium, or the like, can be expected.
摘要:
The present invention addresses the problem of providing a method for specifically measuring CK-MB while avoiding an influence of CK-BB. That is, the present invention relates to "A method for measuring CK-MB in a sample, comprising: a step for reacting the sample with a first antibody against CK-MB, a second antibody against CK-MB which recognizes an epitope different from that recognized by the first antibody, and an anti-CK-B antibody which recognizes a region of the 1st to 100th amino acid from N-terminus of the amino acid sequence of the subunit B of creatine kinase (step 1); a step for measuring an optical change generated by the reaction (step 2); and a step for determining a quantity of CK-MB in the sample on the basis of the result obtained in the step 2 (step 3), and a kit to be used in the method, and a method for avoiding a influence of creatine kinase BB isozyme in measuring CK-MB, comprising treating a sample with the anti-CK-B antibody."
摘要:
It is an object of the present invention to provide a cyanine-based coloring composition having higher heat resistance, as compared with a conventional coloring composition. The present invention relates to a compound represented by the following general formula (1), a polymer having a monomer unit derived from the compound, along with a coloring composition, a coloring composition for a color filter, and a colored resin comprising the above-described compound or the above-described polymer.
摘要:
In the method of assaying an analyte in a sample of the present invention, magnetic silica particles are used. The magnetic silica particles include: silica particles containing 60 to 95% by weight of a superparamagnetic metal oxide that has an average particle size of 1 to 15 nm; and the analyte, an analog of the analyte, or a substance capable of specifically binding to the analyte immobilized on the surface of the silica particles.
摘要:
Disclosed are a method for measuring ²G comprising the steps of bringing a sample into contact with a ²G-binding protein 1 and a ²G-binding protein 2, each comprising an amino acid sequence which is identical or substantially identical to an amino acid sequence shown in any one of SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, or SEQ ID N0:20, and having the ²-glucan binding activity, to form a complex of the ²G-binding protein 1, ²G in the sample and the ²G-binding protein 2, measuring quantity of the complex, and determining ²G concentration in the sample based on the quantity of the complex; a reagent and a kit for use in said method; a novel ²G-binding protein; a nucleic acid molecule encoding the ²G-binding protein; and a method for producing the aforementioned ²G-binding protein.
摘要翻译:公开了一种测量G的方法,包括使样品与G结合蛋白1和G结合蛋白2接触的步骤,每个包含与SEQ ID NO:1所示的氨基酸序列相同或基本相同的氨基酸序列 SEQ ID NO:4,SEQ ID NO:6,SEQ ID NO:8,SEQ ID NO:10,SEQ ID NO:14,SEQ ID NO:16,SEQ ID NO:18或SEQ ID NO: 20,并具有β-葡聚糖结合活性,形成样品中的G结合蛋白1,G和G结合蛋白2的复合物,测定复合物的量,并基于 复数的数量; 用于所述方法的试剂和试剂盒; 一种新型的G结合蛋白; 编码该结合蛋白的核酸分子; 以及制备上述的G结合蛋白的方法。
摘要:
The present invention relates to a method for purifying a compound selected from a compound represented by the following general formula [1] or [2], a saturated aliphatic hydrocarbon having no substituent, or kerosene: (R represents a halogen atom or a lower alkyl group having 1 to 3 carbon atoms, and n represents an integer of 1 to 5); (R is the same as above, p and q independently represent an integer of 0 to 4, with the proviso that at least one ofp and q is not 0), comprising: removing a impurity contained in said compound by a first step comprising contacting said compound with ozone gas, and then a second step comprising contacting said compound with an adsorbent selected from zeolite or a basic adsorbent.