公开(公告)号：EP1992638A1

公开(公告)日：2008-11-19

申请号：EP07711100.3

申请日：2007-02-23

申请人： Centro De Ingenieria Genetica Y Biotecnologia

发明人： GUERRA VALLESPÍ, Maribel ,  TORRÉNS MADRAZO, Isis, del Carmen ,  REYES ACOSTA, Osvaldo ,  GARAY PÉREZ, Hilda, Elisa ,  GUILLÉN NIETO, Gerardo, Enrique ,  ACEVEDO CASTRO, Boris, Ernesto ,  UBIETA GÓMEZ, Raimundo

IPC分类号： C07K14/435 ,  A61K38/17

CPC分类号： C07K14/43509 ,  A61K38/00

摘要： The present invention is related to the development of peptides derived from the sequence HYRIKPTFRRLKWKKYKGKFW, showing aminoacids substitutions, these peptides unable to bind to the lipopolysaccharide and bearing anti-tumoral and immunomodulatory capacities. These peptides alone or in combination are useful to treat cancer, and also in synergy with conventional therapies.

摘要翻译： 本发明涉及来自序列HYRIKPTFRRLKWKKYKGKFW的肽的开发，显示出氨基酸取代，这些肽不能结合脂多糖并具有抗肿瘤和免疫调节能力。 单独或组合使用这些肽可用于治疗癌症，并且与常规疗法协同作用。

公开(公告)号：EP1967212A2

公开(公告)日：2008-09-10

申请号：EP06817995.1

申请日：2006-11-20

申请人： CENTRO DE INGENIERIA GENETICA Y BIOTECNOLOGIA (CIGB)

发明人： RAMÓN HERNÁNDEZ, José, Ángel ,  CASTRO ODIO,Fidel, Raúl ,  SÁEZ MARTÍNEZ, Vivian, María ,  PÁEZ MEIRELES, Rolando ,  FERNÁNDEZ SÁNCHEZ, Eduardo

IPC分类号： A61K47/48

CPC分类号： A61K47/60

摘要： A polymeric dendrimer-like structure with four branches of monomethoxypolyethylene glycol that can be represented as:

The carboxylic group of the previous structure can be functionalized for the production of conjugates of pharmaceutical interest. The binding of this dendrimer-like polyethylene glycol to therapeutic proteins improves their in vitro and in vivo stability.

摘要翻译： 具有四个分支的单甲氧基聚乙二醇的聚合物树状聚合物样结构，其可以表示为：先前结构的羧基可以官能化以生产药物兴趣的缀合物。 这种树枝状大分子聚乙二醇与治疗性蛋白质的结合改善了其体外和体内稳定性。

公开(公告)号：EP1955706A1

公开(公告)日：2008-08-13

申请号：EP06805255.4

申请日：2006-10-30

申请人： CENTRO DE INGENIERIA GENETICA Y BIOTECNOLOGIA (CIGB)

发明人： PENTON ROL, Giselle ,  CERVANTES LLANOS, Majel ,  PENTÓN ARIAS, Eduardo ,  GARCÍA DEL BARCO HERRERA, Diana ,  VALENZUELA SILVA, Carmen, María ,  LÓPEZ SAURA, Pedro, Antonio ,  GUILLÉN NIETO, Gerardo, Enrique

IPC分类号： A61K38/21 ,  A61K31/40

CPC分类号： A61K31/40 ,  A61K38/212 ,  A61K2300/00

摘要： The present invention consists of the combination of Interferon alpha and C-Phycocyanin (IFN-α/C-Phyco) for obtaining a pharmaceutical preparation for autoimmune disease, allergy and cancer treatments. The anti-inflammatory, immunomodulator, antioxidant, anti-viral, anti-proliferative and anti-tumoral effects, associated to the regulatory T cell inducer effect demonstrated in this invention is the rationale for the use of the IFN-α/C-Phyco combination in these diseases.

摘要翻译： 本发明由用于获得用于自身免疫疾病，过敏和癌症治疗的药物制剂的干扰素α和C-藻蓝蛋白（IFN-±/ C-Phyco）的组合组成。 与本发明中证明的调节性T细胞诱导物效应相关的抗炎，免疫调节剂，抗氧化剂，抗病毒，抗增殖和抗肿瘤作用是使用IFN-β/ C-Phyco组合 在这些疾病中。

公开(公告)号：EP1787653A2

公开(公告)日：2007-05-23

申请号：EP05762122.9

申请日：2005-07-05

申请人： CENTRO DE INGENIERIA GENETICA Y BIOTECNOLOGIA (CIGB)

发明人： ABRAHANTES PEREZ, María del Carmen, Calle 186 ,  REYES GONZÁLEZ, Jesús, Calle 186 ,  VELIZ RÍOS, Gloria, Calle 140 ,  MARTÍNEZ DÍAZ, Eduardo, Calle 186 ,  GASMURI GONZÁLEZ, Caridad Anais ,  GARCÍA SUÁREZ, José, Calle 186 ,  BEQUET ROMERO, Mónica ,  GONZÁLEZ LÓPEZ, Luis Javier, Calle 186 ,  CASTELLANOS SERRA, Lila Rosa, Kohly 51. ,  SELMAN-HOUSEIN SOSA, Manuel ,  GÓMEZ RIERA, Raúl ,  GAVILONDO COWLEY, Jorge Victor, Calle G

IPC分类号： A61K38/16 ,  C07K14/265 ,  A61P35/00 ,  A61P31/04

CPC分类号： A61K38/16

摘要： The present invention is related to a composition capable of inhibiting the growth of tumoral cells of different histological origins and of activated endothelial cells. The components of said compositions are polypeptide fragments of the serralisins, corresponding to the C-terminal fragment, from the internal metionine trough the end of the molecule, which could be combined among them and optionally with the prodigiosins that potentiate the antitumoral effect of the composition. The prodigiosins in the composition could be at a concentration of 0.1 - 100 nM. The anti-proliferative action of this composition is mediated by apoptotic mechanism. It's "in vivo" administration has antitumoral, antiangiogenic and protective effect against malignant tumors.

摘要翻译： 本发明涉及能够抑制不同组织起源和活化的内皮细胞的肿瘤细胞生长的组合物。 所述组合物的组分是对应于来自分子末端的内部甲硫氨酸的对应于C末端片段的血清素的多肽片段，其可以在它们之间并且任选地与促进组合物的抗肿瘤作用的生殖细胞因子组合 。 组合物中的生殖细胞可以是0.1-100nM的浓度。 该组合物的抗增殖作用由凋亡机制介导。 “体内”治疗对恶性肿瘤具有抗肿瘤，抗血管生成和保护作用。

公开(公告)号：EP1785729A2

公开(公告)日：2007-05-16

申请号：EP06076889.2

申请日：2006-10-16

申请人： CENTRO DE INGENIERIA GENETICA Y BIOTECNOLOGIA (CIGB)

发明人： Puente, Aniel Sanchez ,  Gonzalez Lopez, Luis Javier ,  Gil Valdes, Jeovanis ,  Betancourt Nunez, Lazaro Hiram ,  Besada Perez, Vladimir Armando ,  Fernandez de Cossio Dorta-Duque, Jorge ,  Alvarez Gil, Félix Modesto ,  Padron Palomares, Gabriel Ramon

IPC分类号： G01N33/68

CPC分类号： G01N33/6812 ,  G01N33/6842 ,  G01N33/6848

摘要： The present invention describes a method that combines the blocking of primary amino groups and the cation exchange chromatography, to simplify peptide mixtures that can be generated or not by proteolytic or chemical treatments. This method allows the selective isolation of an average of 4 multiply-charged peptides (RH peptides) per protein and the study of 90% of the proteins of the analyzed proteomes. It is applicable to studies of quantitative proteomics without the usage of two-dimensional electrophorsis, it is compatible with any type of isotopic labeling and it is very useful to determine the differential expression of proteins present in multiple conditions (3 to 6 conditions) when using different isotopic labeling in a single experiment. The chromatographic system used also allows the fractionation fraction of the RH peptides to achieve the identification of a greater number of proteins.

摘要翻译： 本发明描述了做了方法结合伯氨基和阳离子交换层析的阻塞，为了简化肽混合物也可以通过蛋白水解或化学处理来产生与否。 此方法允许将平均每蛋白4多电荷肽（RH肽）和所分析的蛋白质组中的蛋白质的90％的研究的选择性分离。 它适用于无二维electrophorsis的使用定量蛋白质组学研究中，它是与任何类型的同位素标记的兼容，它是非常有用的确定性矿存在于多个条件（3至6个条件）的蛋白质的差异表达当使用 不同同位素标记在单次实验。 使用这样的色谱系统允许RH肽的分馏部分，以实现蛋白的更大数量的识别。

公开(公告)号：EP1704778A1

公开(公告)日：2006-09-27

申请号：EP05077830.7

申请日：2005-12-09

申请人： CENTRO DE INGENIERIA GENETICA Y BIOTECNOLOGIA (CIGB)

发明人： Aguiar Santiago, Jorge Agustin ,  Fernandez Masso, Julio Raúl ,  Mendoza Mari, Yssel ,  Vasquez Marcos, Maria Mercedes ,  Cruz Leo, Silian ,  Velazquez Perez, Luis Clodoaldo ,  Herrera Martinez, Luis Saturnino

IPC分类号： A01K67/027 ,  C12N15/85 ,  C12N5/10 ,  C07K14/47

CPC分类号： C12N15/8509 ,  A01K67/0275 ,  A01K2227/105 ,  A01K2267/0356

摘要： The present invention is related with the neurobiology and the transgenic animals, more specifically with obtaining a model to study the SCA2 genetic disease. The genome of one mice line has been modified through the introduction of a DNA segment that contains the necessary information for the synthesis of the human ataxin 2. In particular the invention is related with the transgenic mice F066 and the cellular lines able to express the human sca2 gene under the regulation of its self promoter. The F066 transgenic mice reproduce the features of the disease.
The homozygous transgenic mouse line for the new gene was obtained through matting. This transgenic line is useful to study the mechanism that produce the disease and also will permit to prove new therapeutics that contributes to minimize the clinical symptoms in the patients.

摘要翻译： 本发明涉及神经生物学和转基因动物，更具体地涉及获得研究SCA2遗传病的模型。 一条小鼠系的基因组已通过引入含有合成人ataxin2的必需信息的DNA片段进行修饰。特别地，本发明涉及转基因小鼠F066和能够表达人类的细胞系 sca2基因在其自身启动子的调节下。 F066转基因小鼠复制该疾病的特征。 通过消光获得新基因的纯合转基因小鼠品系。 该转基因系可用于研究产生该疾病的机制，并且还将允许证明有助于最小化患者临床症状的新治疗剂。

公开(公告)号：EP0774906B1

公开(公告)日：2006-03-15

申请号：EP95927094.3

申请日：1995-08-10

申请人： CENTRO DE INGENIERIA GENETICA Y BIOTECNOLOGIA

发明人： MENA CAMPOS, Jesus ,  DE LA RIVA DE LA RIVA, Gustavo Alberto ,  VASGUEZ MENDEZ, Ramon, P. ,  FERNANDEZ MACHUCA, Marina ,  COEGO GONZALEZ, Alberto ,  GARCIA GONZALEZ, Melba ,  PIMENTEL VASQUEZ, Eulogio ,  LOPEZ QUESADA, Alina ,  GARCIA GONZALEZ, Rolando ,  ZALDUA GUERRA, Zurima ,  MENCHO PONCE, Juan Diego

IPC分类号： A01N63/00 ,  C12R1/15 ,  C12R1/01

CPC分类号： C12R1/15 ,  A01N63/00 ,  C12R1/01

摘要： New bacterial strains, in particular Sphingobacterium spiritivorum C-926 and Corynobacterium paurometabolum C-924 with useful characteristics in the farming sector due to their nematicidic effect, which can be used in this context for the bio-control of nematodes. Bionematicides capable of effectively controlling phytopathogenic nematode populations in various crops of interest. Useful strains may be isolated from soil taken from areas habitually affected by a high incidence of nematodes, from banana plants showing less severe symptoms of infestation. Strains are selected which are capable of affecting the eggs of the nematode Meloidogyne incognita, in experiments carried out under laboratory conditions. Active strains, such as Sphingobacterium spiritivorum strain C-926 and Corynobacterium paurometabolum strain C-924, are tested to assess the protection which the bacterial preparation, applied directly, can provide to pumpkin and banana plants in relation to Meloidogyne incognita and Radopholus similis. Strains may also be tested against the zoonematodes Haemonchus sp. and Trichostrongylus sp. Control of these parasites allows veterinary use. In addition to the nematodes referred to above, and selected due to the high losses which they incur in crops, the strains may also be effective in the control of populations of Heterodera ssp., Globodera ssp., and Pratylenchus ssp., as well as other species of zoonematodes.

公开(公告)号：EP1577395A1

公开(公告)日：2005-09-21

申请号：EP03767379.5

申请日：2003-12-19

申请人： CENTRO DE INGENIERIA GENETICA Y BIOTECNOLOGIA

发明人： SELMAN-HOUSEIN SOSA, Guillermo ,  SALAZAR RODR GUEZ, Alberto ,  ABREU REMEDIOS, Daymi ,  RAMOS GONZ LEZ, Osmany

IPC分类号： C12N15/82

CPC分类号： C12N15/8216

摘要： An artificial promoter characterized for been a chimerical recombinant DNA molecule such that, when introduced in any class of plant cells, promotes high expression levels of any DNA molecule fused to its 3' end. The basic genetic elements of the molecule described here are: a core promoter with a consensus TATA box, followed by an Exon/Intron/Exon region and a translation enhancer element, all of them artificially constructed. Transcription regulatory elements can be inserted upstream of the promoter here described to confer temporal-, organ- or tissue-specificity to the expression. The designed artificial genetic elements can be functionally inserted between any promoter active in plant cells and any DNA sequence to increase its transcription/translation levels.

公开(公告)号：EP1557468A2

公开(公告)日：2005-07-27

申请号：EP03798037.2

申请日：2003-09-15

申请人： CENTRO DE INGENIERIA GENETICA Y BIOTECNOLOGIA

发明人： SELMAN-HOUSEIN SOSA, Guillermo ,  AGUIAR CABEZA, Eduardo ,  GONZALEZ QUINTERO, Annery, del Carmen ,  RAMOS GONZALEZ, Osmany

IPC分类号： C12N15/82 ,  A01H1/00 ,  A01H5/00

CPC分类号： C12N15/8214

摘要： A DNA vector is provided for stable insertion and expression of heterologous genes in the plastid genomes of any Angiosperm plant cell. In this vector, foreign genes can be inserted in multiple cloning sites located at an artificial intergenic region obtained from the combination of atpB and rbcL operons that belong to plants of different Classes (dicotyledonous and monocotyledonous). The expression cassette is inserted into the plastid genome by homologous recombination between the atpB and rbcL border sequences of the vector with the corresponding homologous regions of plastid genome; in such a way that more than one gene of interest can be expressed under the transcriptional control of the rbcL promoter adjacent to the border region that encodes for the atpB gene.

摘要翻译： 提供DNA载体用于在任何被子植物细胞的质体基因组中稳定插入和表达异源基因。 在该载体中，外源基因可以插入位于人工基因间区域的多个克隆位点，所述人工基因间区域来自属于不同类别（双子叶植物和单子叶植物）的植物的atpB和rbcL操纵子的组合。 表达盒通过载体的atpB和rbcL边界序列与质体基因组的相应同源区域之间的同源重组插入到质体基因组中; 使得可以在与编码atpB基因的边界区相邻的rbcL启动子的转录控制下表达多于一个的感兴趣基因。

公开(公告)号：EP1162266A3

公开(公告)日：2004-01-07

申请号：EP01202009.5

申请日：2001-05-28

申请人： CENTRO DE INGENIERIA GENETICA Y BIOTECNOLOGIA

发明人： Menéndez Diaz, Javier ,  Valdés Prado, Iris, Calle Santa Felicia No. 427 ,  Cabrera Leòn, Nelson

IPC分类号： C12N15/31 ,  C12N15/81 ,  C07K14/39

CPC分类号： C07K14/39 ,  C12N15/815

摘要： This invention relates to the field of recombinant DNA technology. The present invention relates to the isolation of a DNA regulatory region, able to lead the expression of heterologous proteins in Pichia pastoris . In one of its aspects the invention is related to the isolation of a DNA fragment that contains the isocitrate lyase encoding gene (referred here as ICL ) from the yeast Pichia pastoris . In another aspects, this invention describes the use of a fragment derived from the described gene, which lead the expression of a foreign gene, when both are incorporated in a DNA vector and introduced in the host yeast Pichia pastoris. In this way the protein of interest is efficiently produced using this DNA fragment. In the yeast Pichia pastoris the expression of this gene is regulated in response to environmental conditions, such as the growth medium composition. The ICL expression is repressed when a carbon source like glucose is used, and induced when the carbon source present in the medium is ethanol or when the glucose is absent. An additional aspect, we have also isolated a DNA fragment consisting essentially of the Pichia pastoris ICL 3' transcription termination sequence.