PLANT SERINE PROTEASES
    54.
    发明公开

    公开(公告)号:EP3632925A1

    公开(公告)日:2020-04-08

    申请号:EP18198240.6

    申请日:2018-10-02

    摘要: The present invention relates to a genetically modified plant or plant cell derived from a wild-type plant or plant cell, said wild-type plant or plant cell producing at least one serine protease comprising the motif SX 1 RGPX 2 LKPDX 3 X 4 X 5 PGX 6 SGTSMX 7 X 8 PHX 9 PX 10 WSPX 11 AX 12 X 13 SAX 14 MTT (SEQ ID No. 1), wherein X 1 is S, G or A, X 2 is a peptide consisting of 7 to 12 amino acid residues, X 3 is I or L, X 4 is T, I, L or M, X 5 is A or G, X 6 is a peptide consisting of 24 to 28 amino acid residues, X 7 is S or A, X 8 is C or A, X 9 is a peptide consisting of 12 amino acid residues, X 10 is T, E, D, K or H, X 11 is S or A, X 12 is V or I, X 13 is K or R and X 14 is I, L or M, wherein the proteolytic activity of the at least one serine protease in the genetically modified plant or plant cell is reduced compared to its activity in the wild-type plant or plant cell.

    METHOD FOR DETERMINING CONDITIONS FOR CULTIVATION, AND METHOD FOR PRODUCING DESIRED PROTEIN OR DESIRED PEPTIDE

    公开(公告)号:EP4265104A1

    公开(公告)日:2023-10-25

    申请号:EP22739380.8

    申请日:2022-01-11

    摘要: Disclosed is a method for determining conditions for cultivating a plant body expressing a protein of interest or a peptide of interest in a 24-hour cycle in which a dark period and a light period are repeated alternately, including: a step of determining an optimum leaf temperature based on a capability to express a protein of interest or a peptide of interest; and a step of setting light and dark period leaf temperatures and light and dark period time lengths after a plant body is infected with a bacterium or a virus having a polynucleotide encoding the protein of interest or the peptide of interest so as to satisfy the following formula, the following formula being: Dark period leaf temperature (°C) × Dark period time length (hour) + Light period leaf temperature (°C) × Light period time length (hour) = Optimum leaf temperature (°C) × 24 (hour) ± 24.