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公开(公告)号：EP0501821A3
公开(公告)日：1993-03-03
申请号：EP92301721.4
申请日：1992-02-28
申请人： PHARMACIA S.p.A.
CPC分类号： C12N15/86 , A61K38/00 , C07K14/005 , C07K14/815 , C07K2319/00 , C07K2319/02 , C07K2319/035 , C07K2319/33 , C12N2710/14122 , C12N2710/14143 , C12N2760/20222
摘要： The present invention relates to anti-thrombin polypeptides isolated from the leech Hirudinaria manillensis and to processes for their preparation. The polypeptides of the invention may be modified by way of amino acid extension at either or each end, and may be subjected to post-translational modification. The anti-thrombin polypeptides may be prepared by isolating them from the tissue or secretions of the leech Hirudinaria manillensis but they also may be synthetized by recombinant DNA methods. According to this latter aspect, the invention provides DNA sequences, expression vectors and host cell lines for the recombinant preparation of the polypeptides. The anti-thrombin polypeptides of the invention find an useful application in the treatment of venous thrombosis, vascular shunt occlusion and thrombin-induced disseminated intravascular coagulation.
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公开(公告)号：EP0222412B1
公开(公告)日：1992-11-19
申请号：EP86115833.5
申请日：1986-11-14
发明人： Maeda, Susumu , Furusawa, Mitsuru , Marumoto, Yasumasa , Horiuchi, Tadashi , Sato, Yoshinari , Saeki, Yoshiyuki
CPC分类号： C12N15/86 , C07K14/005 , C07K14/56 , C07K14/65 , C07K2319/00 , C07K2319/09 , C07K2319/735 , C07K2319/75 , C12N2710/14122 , C12N2710/14143
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63.发明公开
公开(公告)号：EP0384294A3
公开(公告)日：1991-06-05
申请号：EP90102939.7
申请日：1990-02-15
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/005 , C12N2710/14122 , Y02A40/162
摘要： A gene coded for a polypeptide isolated from an occlusion body of a baculovirus such as Trichoplusia ni granulosis virus possessing a biological activity of enhancing virus infection of host insects by causing rapid degradation of the peritrophic membrane lining the midgut lumen of insects. Novel pesticides incorporating said gene or fragments thereof are also claimed.
摘要翻译： 编码用于分离自杆状病毒（例如粉纹夜蛾）病毒的杆状病毒的闭塞体的多肽的基因，具有通过引起昆虫中肠内脏的内皮细胞膜的快速降解来增强宿主昆虫的病毒感染的生物学活性。 还要求引用所述基因或其片段的新型农药。
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公开(公告)号：EP0385394A3
公开(公告)日：1991-05-22
申请号：EP90103793.7
申请日：1990-02-27
发明人： Yamada, Nobutoshi, Ishihara Sangyo Kaisha Ltd. , Matsuo, Norifusa, Ishihara Sangyo Kaisha Ltd. , Araki, Takaaki, Ishihara Sangyo Kaisha Ltd.
CPC分类号： C12N15/86 , C07K14/005 , C12N2710/14122 , C12N2710/14143
摘要： A polyhedrin gene of Spodoptera litura nuclear polyhedrosis virus (SℓNPV C-411) has the following restriction enzyme cleavage map and about 3 kilobase pairs: At least a part of said polyhedrin gene can be substituted by a gene coding for a useful substance to construct a vector and a recombinant virus, and it is possible to produce a useful substance such as a peptide, protein or glycoprotein by culturing a cell infected with the recombinant virus.
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公开(公告)号：EP0127839A3
公开(公告)日：1986-06-25
申请号：EP84105841
申请日：1984-05-22
发明人： Smith, Gale E. , Summers, Max D.
CPC分类号： C12N15/86 , C07K14/005 , C07K14/52 , C07K2319/00 , C07K2319/02 , C07K2319/09 , C07K2319/61 , C07K2319/735 , C07K2319/75 , C12N2710/14122 , C12N2710/14143 , C12N2710/14151
摘要： A method for producing a recombinant baculovirus expression vector, capable of expressing a selected gene in a host insect cell is disclosed. The method involves cleaving baculovirus DNA to produce a DNA fragment comprising a polyhedrin gene or portion thereof, including a polyhedrin promoter. A recombinant transfer vector is prepared by inserting said DNA fragment into a cloning vehicle and thereafter inserting a selected gene into the thus modified cloning vehicle such that it is under the transcriptional control of the polyhedrin promoter. The recombinant transfer fector is contacted with a baculovirus DNA so as to effect recombination and incorporation of the selected gene into the baculovirus genome. The resultant recombinant baculovirus is then used to infect susceptible insects or cultured insect cells and the protein product from the incorporated selected gene is produced from the infection.
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