Screen to identify a drug for a regenerative drug therapy
    62.
    发明公开
    Screen to identify a drug for a regenerative drug therapy 审中-公开
    筛选以鉴定所述药物用于再生药物治疗

    公开(公告)号:EP2442103A2

    公开(公告)日:2012-04-18

    申请号:EP11170593.5

    申请日:2006-11-30

    发明人: Choo, Yen

    IPC分类号: G01N33/50 C12N15/06

    摘要: A screen to identify a drug for administration to a patient in a regenerative drug therapy that regenerates or repairs a tissue or organ in a human body comprises providing a physiologically relevant human progenitor cell and screening the progenitor cell for a factor that induces differentiation into a cell of the tissue or organ. The physiologically relevant progenitor cell is obtained by:-
    (a) identifying a target phenotype of interest, being a cell of the tissue or organ that is going to be regenerated;
    (b) obtaining cells of a first type;
    (c) determining a differentiation protocol which leads to the appearance of the target phenotype from cells of the first type via a progenitor cell; and
    (d) modifying the protocol such that the differentiation process is stalled at a stage in which the progenitor cells are present, thereby providing said physiologically relevant progenitor cells for use in the screen.

    METHOD OF IDENTIFYING AGENTS WHICH MODULATE THE ACTIVITY OF CALCIUM-ACTIVATED CHLORIDE CHANNEL
    69.
    发明公开
    METHOD OF IDENTIFYING AGENTS WHICH MODULATE THE ACTIVITY OF CALCIUM-ACTIVATED CHLORIDE CHANNEL 有权
    一种用于识别装置,用于调制钙激活氯离子通道的活性

    公开(公告)号:EP2201113A2

    公开(公告)日:2010-06-30

    申请号:EP08839874.8

    申请日:2008-10-17

    IPC分类号: C12N15/06

    摘要: The present invention provides a method of identifying an agent which modulates an activity of a protein, wherein the protein is represented by the amino acid sequence of SEQ ID NO: 1 or has at least 90 % amino acid sequence identity to SEQ ID NO: 1 and transports chloride ions across a cell membrane, comprising: (a) exposing cells which express the protein to the agent; and (b) measuring degree of chloride ion transport in the exposed cells, wherein a change in the degree of chloride ion transport compared to control cells, which express the protein but not exposed to the agent, is indicative of an agent capable of modulating an activity of the protein. The agents identified by this method can be used for the prevention or treatment of various diseases caused by the dysfunction of calcium activated chloride channel.