公开(公告)号：EP2268809A4

公开(公告)日：2013-02-27

申请号：EP09738908

申请日：2009-05-01

申请人： UNIV KYOTO

发明人： YAMANAKA SHINYA ,  OKITA KEISUKE

IPC分类号： C12N15/09 ,  C12N5/074

CPC分类号： C12N5/0696 ,  C12N15/79 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2506/02 ,  C12N2506/13 ,  C12N2506/1307 ,  C12N2506/1361 ,  C12N2510/00

摘要： This invention provides a method of producing an induced pluripotent stem cell comprising the step of introducing at least one kind of non-viral expression vector (more preferably a plasmid vector) incorporating at least one gene that encodes a reprogramming factor into a somatic cell. An induced pluripotent stem cell wherein no exogenous genes induced is integrated into the cellular genome is also provided.

摘要翻译： 产生诱导性多能干细胞的方法包括将一种或多种非病毒表达载体引入体细胞中。 载体包括Oct家族基因，Klf家族基因，Sox家族基因，Myc家族基因，Lin家族基因和Nanog基因中的一种或多种。 然后将体细胞在支持多能干细胞的培养基中培养。 一种或多种引入的非病毒表达载体的至少一部分基本上不整合到染色体中。

公开(公告)号：EP2462225A1

公开(公告)日：2012-06-13

申请号：EP10806584.8

申请日：2010-08-06

申请人： Kyoto University

发明人： OKITA, Keisuke ,  NAKAGAWA, Masato ,  YAMANAKA, Shinya

IPC分类号： C12N15/09 ,  C12N5/10

CPC分类号： C12N15/85 ,  C12N5/0696 ,  C12N2501/40 ,  C12N2501/48 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2502/13 ,  C12N2506/1361 ,  C12N2510/00 ,  C12N2800/108 ,  C12N2800/30

摘要： Provided is a method of producing an iPS cell, comprising bringing (a) Oct3/4 or a nucleic acid that encodes the same, (b) Klf4 or a nucleic acid that encodes the same, and (c) Sox2 or a nucleic acid that encodes the same, as well as (d1) L-Myc or a nucleic acid that encodes the same and/or (d2) a functional inhibitor of p53, into contact with a somatic cell. It is preferable that (a) a nucleic acid that encodes Oct3/4, (b) a nucleic acid that encodes Klf4, (c) a nucleic acid that encodes Sox2, (d1) a nucleic acid that encodes L-Myc and (e) a nucleic acid that encodes Lin28 or Lin28b be inserted into an episomal vector having loxP sequences placed in the same orientation on the 5′ and 3′ sides of a vector constituent essential for the replication of the vector, that (d2) a nucleic acid that encodes an shRNA against p53 be inserted into a vector ensuring transient expression (plasmid vector and the like), and that all these nucleic acids be transferred to a somatic cell.

公开(公告)号：EP2119769A4

公开(公告)日：2011-01-19

申请号：EP08710780

申请日：2008-02-05

申请人： UNIV MATSUMOTO DENTAL

发明人： OZAWA HIDEHIRO ,  YAMAKI MARIKO ,  ASASHIMA MAKOTO ,  EBINA SATOSHI

IPC分类号： A61L27/00 ,  A61P17/14 ,  C12N5/071 ,  C12Q1/02 ,  G01N33/15 ,  G01N33/50

CPC分类号： G01N33/5082 ,  C12N5/0627 ,  C12N2501/105 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2506/02 ,  C12N2506/1361 ,  C12N2533/54

公开(公告)号：EP2633870B1

公开(公告)日：2018-08-01

申请号：EP12157470.1

申请日：2012-02-29

申请人： Technische Universität Berlin

发明人： Lauster, Roland ,  Binder, Jennifer ,  Rosowski, Mark ,  Marx, Uwe

IPC分类号： A61L27/38

CPC分类号： C12N5/0654 ,  A61K35/32 ,  A61L27/3804 ,  A61L27/3821 ,  A61L27/3865 ,  A61L2430/12 ,  C12N5/0664 ,  C12N2502/094 ,  C12N2506/1361 ,  C12N2513/00 ,  G01N33/5044 ,  G01N33/6887

摘要： The present invention is directed to a method of preparing an artificial tooth primordium in vitro, comprising the steps: a) providing isolated mesenchymal dental pulp cells; and b) culturing the mesenchymal dental pulp cells under non-adherent conditions to form a cell aggregate representing an artificial tooth primordium; as well as to an artificial tooth primordium derived therefrom.

公开(公告)号：EP2832851A4

公开(公告)日：2015-09-02

申请号：EP13769656

申请日：2013-03-28

申请人： QUARRYMEN CORP

发明人： UEDA MINORU

IPC分类号： C12N5/10 ,  A61K9/06 ,  A61K9/08 ,  A61K9/12 ,  A61K9/14 ,  A61K9/70 ,  A61K35/12 ,  A61P1/02 ,  A61P9/00 ,  A61P17/02 ,  A61P19/00 ,  A61P25/00 ,  A61P25/08 ,  A61P25/16 ,  A61P25/18 ,  A61P25/24 ,  A61P25/28 ,  A61P31/00 ,  A61P31/14 ,  C12N15/09

CPC分类号： C12N5/0664 ,  A61K9/0014 ,  A61K9/0043 ,  A61K35/28 ,  A61K35/32 ,  A61K35/54 ,  A61K38/1833 ,  A61K38/1841 ,  A61K38/1866 ,  A61K38/30 ,  C12N5/0696 ,  C12N15/85 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2506/1346 ,  C12N2506/1361

摘要： The purpose of the present invention is to provide immortalized stem cells, which produce a growth factor capable of regenerating various kinds of tissues that have been damaged by a variety of causes, and a method for producing the aforesaid immortalized stem cells. Another purpose of the present invention is to provide a medicinal composition and a medicinal preparation for restoring damaged tissues, and a method for the percutaneous absorption of a culture supernatant. Provided are immortalized stem cells that are obtained by isolating stem cells selected from the group consisting of mammalian mesenchymal cells, an embryo at the early stage of the development and somatic cells, first culturing the cells to give first stage culture cells, transferring four kinds of genes into the first stage culture cells to give transgenic cells, and selecting the desired immortalized stem cells from among the transgenic cells using the expression of STRO-1 as an index. Also provided are a medicinal composition and a medicinal preparation for restoring damaged tissues which comprise a culture supernatant of the immortalized stem cells as the active ingredient.

公开(公告)号：EP2119769B1

公开(公告)日：2013-03-27

申请号：EP08710780.1

申请日：2008-02-05

申请人： Matsumoto Dental University

发明人： OZAWA, Hidehiro ,  YAMAKI, Mariko ,  ASASHIMA, Makoto ,  EBINA, Satoshi

IPC分类号： C12N5/071 ,  G01N33/50

CPC分类号： G01N33/5082 ,  C12N5/0627 ,  C12N2501/105 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2506/02 ,  C12N2506/1361 ,  C12N2533/54

公开(公告)号：EP1619949A4

公开(公告)日：2007-07-04

申请号：EP03724136

申请日：2003-04-19

申请人： US GOV HEALTH & HUMAN SERV

发明人： SHI SONGTAO ,  ROBEY PAMELA GEHRON ,  MIURA MASAKO ,  GRONTHOS STAN

IPC分类号： A01N63/00 ,  A01N65/00 ,  A61K48/00 ,  C12N5/00 ,  C12N5/02 ,  C12N5/077

CPC分类号： A61K35/30 ,  A61K35/12 ,  A61K47/02 ,  C12N5/0602 ,  C12N5/0618 ,  C12N5/0654 ,  C12N5/0664 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2506/1361 ,  C12N2533/54

摘要： The invention generally relates to postnatal dental stem cells and methods for their use. More specifically, the invention relates in one aspect to postnatal dental pulp stem cells, use of the cells to generate dentin, and differentiation of the cells. In another aspect, the invention relates to human postnatal deciduous dental pulp multipotent stem cells, use of the cells to generate dentin, and differentiation of the cells.

公开(公告)号：EP1705245A1

公开(公告)日：2006-09-27

申请号：EP05102356.2

申请日：2005-03-23

申请人： Stiftung CAESAR

发明人： Thie, Michael, Dr. ,  Degistirici, Özer, Dr.

IPC分类号： C12N5/06 ,  C12N5/08 ,  A61P25/00

CPC分类号： C12N5/0623 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/13 ,  C12N2506/1361

摘要： Subject of the invention is a method for generating neural stem cells in vitro, wherein dental follicle stem cells are isolated from tissue of the dental follicle of tooth or wisdom tooth and differentiated to the neural stem cells.

摘要翻译： 本发明的主题是体外产生神经干细胞的方法，其中将牙囊干细胞从牙齿或智齿的牙髓的组织中分离并分化成神经干细胞。

公开(公告)号：EP2408904B1

公开(公告)日：2017-10-18

申请号：EP10753012.3

申请日：2010-03-22

申请人： Mesoblast, Inc.

发明人： ITESCU, Silviu

IPC分类号： C12N5/074 ,  C12N15/86 ,  G01N33/50

CPC分类号： C12N5/0696 ,  C12N15/86 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2506/1353 ,  C12N2506/1361 ,  C12N2506/1384 ,  C12N2510/00 ,  C12N2740/15043 ,  G01N33/5073

公开(公告)号：EP3093023A4

公开(公告)日：2017-10-11

申请号：EP15740157

申请日：2015-01-23

申请人： QUARRYMEN & CO INC

发明人： UEDA MINORU

IPC分类号： A61K35/32 ,  A61K38/18 ,  A61K38/19 ,  A61K38/30 ,  A61P35/00

CPC分类号： A61K38/30 ,  A61K35/12 ,  A61K35/32 ,  A61K38/1841 ,  A61K38/1866 ,  A61K38/195 ,  C12N5/0664 ,  C12N5/0696 ,  C12N2500/02 ,  C12N2500/90 ,  C12N2500/99 ,  C12N2501/105 ,  C12N2501/15 ,  C12N2501/165 ,  C12N2501/21 ,  C12N2501/60 ,  C12N2501/998 ,  C12N2506/1361 ,  C12N2510/04

摘要： The purpose of the present invention is to provide a novel anticancer therapeutic having a high effect against solid cancers, lacking the side effects of chemotherapeutics, and being unlikely to cause resistance. Provided is a pharmaceutical composition for cancer treatment prepared by a method having: a stem cell production step for making immortalized stem cells by introducing four types of genes into deciduous tooth dental pulp stem cells obtained from the dental pulp of mammals; and a condition medium preparation step for culturing the immortalized stem cells for a predetermined length of time in serum-free medium at 23-27°C under conditions of low oxygen concentration at an oxygen concentration of from 0.5% to less than 20%, the pharmaceutical composition containing 1.5 times or more of insulin-like growth factor (IGF-1) and vascular endothelial growth factor (VEGF) than is contained in condition medium prepared when culturing at an oxygen concentration of 20% and otherwise identical conditions.