摘要:
The disclosed technology brings histopathology into the operating theatre, to enable real-time intra-operative digital pathology. The disclosed technology utilizes confocal imaging devices image, in the operating theatre, “optical slices” of fresh tissue—without the need to physically slice and otherwise process the resected tissue as required by frozen section analysis (FSA). The disclosed technology, in certain embodiments, includes a simple, operating-table-side digital histology scanner, with the capability of rapidly scanning all outer margins of a tissue sample (e.g., resection lump, removed tissue mass). Using point-scanning microscopy technology, the disclosed technology, in certain embodiments, precisely scans a thin “optical section” of the resected tissue, and sends the digital image to a pathologist rather than the real tissue, thereby providing the pathologist with the opportunity to analyze the tissue intra-operatively. Thus, the disclosed technology provides digital images with similar information content as FSA, but faster and without destroying the tissue sample itself.
摘要:
A video-confocal microscopy method for creating an image of an optical section (π) of a sample (99), provides, in one aspect of the invention, illuminating the sample (99) with illumination beams (19) concentrated on spots (x,y) arranged in an illumination pattern (18) in an illumination plane (α0) at the optical section (π); translationally parallel moving the pattern (18) of spots (x,y) to a plurality of positions in the illumination plane; it also provides for each position (u,v) of the illumination pattern (18), receiving light (21) returned by the sample (99) by reflection and/or transmission and/or phosphorescence, and detecting raw images (52), each having a light intensity distribution Iu,v(x,y) on said image detector.
摘要:
A confocal optical scanner according to one aspect of the present invention includes an optical filter. The optical filter includes a plate-shaped body including a first surface and a second surface opposite to the first surface. The first surface has a plurality of pinholes. The optical filter further includes a plurality of first microlenses on the second surface. The first microlenses are disposed nearly coaxially with the pinholes, respectively.
摘要:
A microscope apparatus includes a light source configured to emit a coherent illuminating light, an optical system configured to irradiate a specimen with the illumination light, and a detector configured to form an image based on a light generated from the specimen by the illumination light that irradiates the specimen. The optical system is configured to project a plurality of focal points of the illumination light on the specimen, and allow the plurality of focal points to interfere with each other while changing phases of the plurality of focal points.
摘要:
A video-confocal microscopy method for creating an image of an optical section (π) of a sample (99), provides, in one aspect of the invention, illuminating the sample (99) with illumination beams (19) concentrated on spots (x,y) arranged in an illumination pattern (18) in an illumination plane (α0) at the optical section (π); translationally parallel moving the pattern (18) of spots (x,y) to a plurality of positions in the illumination plane; it also provides for each position (u,v) of the illumination pattern (18), receiving light (21) returned by the sample (99) by reflection and/or transmission and/or phosphorescence, and detecting raw images (52), each having a light intensity distribution Iu,v(x,y) on said image detector.
摘要:
In an episcopic multiplexed confocal scanning microscope system (400, 450, 500, 600, 700, 800, 900, 1000), a spatial filter (432, 514, 632) is placed at a conjugate plane (448) to a back aperture plane (446) at a back aperture of an objective lens (412). The spatial filter is used to combine excitation light and emission light from a multiplexed confocal microscope module (406).
摘要:
Die Erfindung betrifft eine Vorrichtung sowie ein Verfahren zur dreidimensionalen optischen Abtastung einer Probe. Der Abtastprozess erlaubt es, dreidimensionale Daten der zu untersuchenden Probe zu erhalten, ähnlich dem Verfahren der Konfokalen Mikroskopie, jedoch unter Nutzung von strukturierter Beleuchtung. Ein besonderer Vorteil besteht darin, dass die axiale Abtastung sowie die Laterale Abtastung durch einen einzigen Aktuator vorgenommen werden. Durch die Anwendung eines Weitfeldverfahrens und die Möglichkeit zur Abtastung mit kontinuierlicher Geschwindigkeit können hohe Abtastraten erzielt werden. Des Weiteren betrifft die Erfindung die Ermöglichung eines kontinuierlichen Scanprozesses zur kontinuierlichen Abtastung von Flächen, welche größer als das Gesichtsfeld der genutzten Optik sind. Hierfür wird eine besonders effiziente Form der Abtastung vorgeschlagen, bei welcher durch Verkippung der Fokusebene in Bezug auf die Scanbewegung mit nur einem Aktuator gleichzeitig eine Tiefenabtastung der Probe erzielt wird.