Abstract:
An apparatus and method for characterizing luminescent entities by excitation comprising: a substrate (6) being in contact with a solution comprising luminescent entities; a source of electromagnetic radiation (4) providing at least a primary beam of radiation (8); an objective (5); a first optical element (1) capable of transforming the intensity profile of the primary beam into an arbitrary secondary intensity profile (distribution); a second optical element (2) capable of separating (discriminating) radiation by wavelength; and a detector (7); where the arbitrary secondary intensity profile is focused on the back focal plane (x) of the objective such that at least a collimated beam is obtained capable of creating an evanescent field on the side of the substrate (6) where the solution comprising luminescent entities are located, wherein the evanescent field excites the luminescent entities thereby creating emission radiation separated by the first optical element (1) and captioned by the detector (7).
Abstract:
The invention relates to a microscope for raster-free, confocal imaging of a sample arranged in a sample space (2), the microscope having: - an illumination device that has a light source group (4; 20) of individually switchable light sources (5; 21); - a detector device (3); - a pinhole device comprising a pinhole array (8; 26), said array having a plurality of pinhole elements (13; 27) one alongside another, wherein one pinhole element (13; 27) is present for each light source (5; 21); and - an optical unit (6, 7, 9, 11) that causes each pinhole element (13: 27) to be irradiated with light from a single one of the light sources (5; 21) in the light source group (4; 20) and confocally illuminates a single spot (14) situated in the sample space (2). One of the single spots (14) is associated with each pinhole element (13; 27) and the single spots (14) are situated one alongside another in the sample space (2) in relation to an incidence direction of the light, and the optical unit (11, 9, 7, 15, 17) images the single spots (14) through the pinhole device confocally onto the detector device (3).
Abstract:
The present invention relates to a method of obtaining multiple images with a spatially varying periodic fringe pattern produced by a digital light projector (10) that is spatially and temporally synchronized to the rolling shutter exposure of a two-dimensional pixel array detector (33). Two or more images obtained with spatially phase-shifted fringe patterns are combined to form a composite image with the spatial fringes and other unwanted scattered light removed.
Abstract:
The invention relates to a nonlinear optical microscope using a Bessel laser beam so as to image a sample with good lateral resolution and extended field depth. This microscope incorporates a slide scanner. The sample is disposed on a slide, which is loaded automatically onto a motorized platen, so that the laser beam can sweep the entire slide. The useful signals originating from the sample are combined with the conventional image of the slide scanner so as to obtain a global image which clearly reveals, with good resolution, elements of interest.
Abstract:
The invention provides imaging apparatus and methods useful for obtaining a high resolution image of a sample at rapid scan rates. A rectangular detector array having a horizontal dimension that is longer than the vertical dimension can be used along with imaging optics positioned to direct a rectangular image of a portion of a sample to the rectangular detector array. A scanning device can be configured to scan the sample in a scan-axis dimension, wherein the vertical dimension for the rectangular detector array and the shorter of the two rectangular dimensions for the image are in the scan-axis dimension, and wherein the vertical dimension for the rectangular detector array is short enough to achieve confocality in a single axis.
Abstract:
A digital imaging device comprising a light source, a pixel array detector having a rolling shutter functionality, a spatial light modulator configured to produce one or more modulation patterns during a frame exposure of the pixel array detector, and at least one timing signal configured to control a spatial-temporal relationship between a rolling shutter of the pixel array detector and the one or more modulation patterns provided by the spatial light modulator.
Abstract:
Three or more devices can be connected to a microscope and can be used simultaneously. Provided is a microscope connecting unit (120) including a microscope connection port (121) that is connected to a microscope (10) used to observe a sample; three or more unit connection ports (123A, 123B, and 123C) to which a stimulating unit (70) that irradiates the sample with light or a confocal observation unit (50) or an image capturing unit (110) that detects light generated at the sample is connectable; and two or more light-path combining units (127A and 127B) that are disposed between the microscope connecting port (121) and the unit connection ports (123A, 123B, and 123C) and that combine light paths optically connecting the microscope (10) with the confocal observation unit (50), the stimulating unit (70), and the image capturing unit (110).
Abstract:
A method of imaging a sample comprises the steps of: -providing S1 a reference array of spots 104, -illuminating the sample 106 with the reference array of spots 104 and acquiring S2 at least one sample image IMSi comprising a sample related array of spots 107 resulting from the reference array of spots interacting with the sample 106, -determining S3 a spot characterizing parameter for each of a plurality of sample related spots, and -constructing S4 an image of the sample IMs By plotting the spot characterizing parameter for each of the plurality of sample related spots at the respective sample related spot position.
Abstract:
Microscope confocal à balayage comprenant par exemple un miroir galvanométrique (1507) pour balayer des microlentilles (1509) à l'aide d'un faisceau d'éclairage (FX)