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    Visual discrimination qualitative enzyme assay
    5.
    发明公开
    Visual discrimination qualitative enzyme assay 失效
    视觉辨别质量测定

    公开(公告)号：EP0348211A3

    公开(公告)日：1990-11-28

    申请号：EP89306353.7

    申请日：1989-06-23

    申请人： MICROGENICS CORPORATION

    发明人： Khanna, Pyare L. ,  Choate, Glenda L.

    IPC分类号： G01N33/543 ,  G01N33/545 ,  G01N33/58

    CPC分类号： G01N33/581 ,  G01N33/558

    摘要： A β-galactosidase complementation assay for determining the presence of an analyte which is a member of a specific binding pair (sbp) in a sample is provided, which assay permits visual discrimination between those samples wherein the analyte is present above a predetermined, threshold concentration. The method comprises combining the sample with a hydro­phobic enzyme donor- (ED-) analyte conjugate and the complementary member of the sbp to form an sbp complex-­containing assay medium. A small volume of the assay medium is spotted onto an enzyme acceptor (EA) affixed to a bibulous, solid support, followed by development with enzyme substrate solution. A substantially larger area is detectable when analyte in the sample is below as compared to above a threshold concentration. The assay is particularly useful in field testing applications such as determining the presence of antibiotics in milk, toxins in water, or drugs in serum or urine. Kits facilitating the method are also provided.

    Enzyme quantitation wicking assay
    6.
    发明公开
    Enzyme quantitation wicking assay 失效
    Enzym-Quantifizierstest auf einem Docht。

    公开(公告)号：EP0376707A2

    公开(公告)日：1990-07-04

    申请号：EP89313626.7

    申请日：1989-12-28

    申请人： MICROGENICS CORPORATION

    发明人： Khanna, Pyare L. ,  Choate, Glenda L.

    IPC分类号： G01N33/542 ,  G01N33/58 ,  G01N33/558 ,  G01N33/78

    CPC分类号： G01N33/558 ,  G01N33/542 ,  G01N33/581

    摘要： Diagnostic assays are provided comprising complementary enzyme fragments of β-galactosidase, where one of the fragments is bound to a support and the other fragment is conjugated to an immunologically cross-reactive epitope of the analyte or complementary to an analyte receptor. One can achieve a determin­ation of ligand, by providing for competition between the ligand analyte and the conjugate for receptor, where binding to the receptor allows for discrimination between complexed enzyme fragment conjugate and uncomplexed enzyme fragment conjugate. The assay medium is then allowed to wick onto a support to which the complementary fragment is substantially uniformly bound in the detection region. The support is then developed, where color formation from the substrate in the detection region is used as a measure of the presence of analyte in the sample.

    摘要翻译： 提供诊断测定法，其包括β-半乳糖苷酶的互补酶片段，其中一个片段与支持物结合，另一个片段与分析物的免疫交叉反应性表位缀合或与分析物受体互补。 通过提供配体分析物和受体结合物之间的竞争，可以实现配体的测定，其中与受体的结合允许区分复合的酶片段缀合物和未复合的酶片段缀合物。 然后允许测定培养基吸收到在检测区域中基本上均匀结合的互补片段的载体上。 然后开发支持体，其中使用检测区域中底物的颜色形成作为样品中分析物存在的量度。

    Receptor preincubation enzyme assay
    7.
    发明公开
    Receptor preincubation enzyme assay 失效
    受体预温育酶试验

    公开(公告)号：EP0327312A2

    公开(公告)日：1989-08-09

    申请号：EP89300928.2

    申请日：1989-01-31

    申请人： MICROGENICS CORPORATION (a Delaware corporation)

    发明人： Khanna, Pyare L. ,  Friedman, Stephen B. ,  Dworschak, Robert T.

    IPC分类号： G01N33/58 ,  G01N33/535 ,  G01N33/94 ,  G01N33/78

    CPC分类号： G01N33/581 ,  G01N33/542

    摘要： A β-galactosidase complementation immunoassay method for determining the concentration of an analyte in a sample is provided. The method is based on com­bining a preformed enzyme donor- (ED-) analyte conju­gate/anti-analyte antibody complex with analyte present in the sample. The method comprises reacting a β-galactosidase ED-analyte conjugate/anti-analyte anti­body complex, a β-galactosidase enzyme acceptor (EA), enzyme substrate and sample and determining the rate of enzyme-catalyzed reaction as indicative of the amount of analyte present in the sample. Usually, the EA is added to the reaction mixture after a time for the anti-analyte antibody of the complex to react with analyte present in the sample.
    In a particular embodiment, the method is used to determine the amount of digoxin in a serum sample. In another embodiment the method is used to determine the amount of T3 in a sample. Kits facilitating the method are also provided.

    摘要翻译： 提供了用于确定样品中分析物浓度的β-半乳糖苷酶互补免疫测定方法。 该方法基于将预先形成的酶供体 - （ED-）分析物缀合物/抗分析物抗体复合物与样品中存在的分析物组合。 该方法包括使β-半乳糖苷酶ED-分析物缀合物/抗分析物抗体复合物，β-半乳糖苷酶酶接受体（EA），酶底物和样品反应，并确定酶催化反应的速率作为存在的分析物的量的指示 在样本中。 通常，在复合物的抗分析物抗体与样品中存在的分析物反应一段时间之后，将EA添加到反应混合物中。 在一个具体的实施方案中，该方法用于确定血清样品中地高辛的量。 在另一个实施方案中，该方法用于确定样品中T3的量。 还提供了便于该方法的试剂盒。

    Homogeneous T-3 or T-4 uptake assay method and kit
    8.
    发明公开
    Homogeneous T-3 or T-4 uptake assay method and kit 失效
    确定T-3或T-4摄取和组合物的为此均相方法。

    公开(公告)号：EP0310430A2

    公开(公告)日：1989-04-05

    申请号：EP88309122.5

    申请日：1988-09-30

    申请人： MICROGENICS CORPORATION

    发明人： Picone, Teresa K. ,  Friedman, Stephan B.

    IPC分类号： G01N33/78

    CPC分类号： G01N33/581 ,  G01N33/78

    摘要： The present invention provides an improved assay for measuring thyroxine uptake by thyroxine bind­ing globulin in which thyroxine binding globulin (TBG) activity is measured directly. The method comprises combining a sample in an aqueous solution with an enzyme donor (ED) conjugated to an analogue of poly­iodothyronine that competes with thyroxine for thy­roxine binding globulin binding sites. An enzyme acceptor (EA) characterized by providing a modulated enzyme activity in relation to the amount of TBG activity is combined with an enzyme donor and sample in an aqueous solution. The amount of enzyme activity in comparison to a control solution having a known amount of available TBG binding sites is determined.

    摘要翻译： 本发明改进的测定法提供用于其中甲状腺素结合球蛋白（TBG）活性由甲状腺素结合球蛋白测量甲状腺素摄取直接测量。 该方法包括在与缀合至酶供体（ED）的wässrige溶液在polyiodothyronine的类似物的竞争对手甲状腺素甲状腺素结合球蛋白结合位点相结合的样品并。 通过提供相对于调制的酶活性，以TBG活性的量为特征的酶受体（EA）与在酶供体合并，并在wässrige溶液样本。 酶活性相比于对照溶液具有可用的TBG结合位点的已知量的量是确定的开采。