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公开(公告)号：EP1212438B1
公开(公告)日：2006-05-17
申请号：EP00954241.6
申请日：2000-08-25
CPC分类号： C07K14/415 , C12N15/8234
摘要： Novel methods for the expression of non-native genes in flax seeds and the seeds of other plant species are provided. The methods involve the use of seed-specific promoters obtained from flax. Additionally provided are novel flax seed-specific promoters, chimeric nucleic acid constructs comprising novel flax seed-specific promoters, transgenic plant cells, transgenic plants and transgenic plant seeds containing novel flax seed-specific promoters. The promoters and methods are useful, for example, for altering the seed oil and protein composition in flax seed or other plant seeds.
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公开(公告)号：EP0636179B1
公开(公告)日：2004-09-22
申请号：EP92908088.5
申请日：1992-04-15
发明人： MOLONEY, Maurice, M.
CPC分类号： C07K14/415 , C07K14/61 , C07K14/8139 , C07K2319/02 , C07K2319/50 , C07K2319/61 , C12N9/6483 , C12N15/8234 , C12N15/8241 , C12N15/8242 , C12N15/8257 , C12Y304/23004
摘要： Methods and compositions for use therein are described for expressing a polypeptide of interest in a seed cell as a fusion protein with an oil body protein. By this means, the fusion protein is targeted to the oil bodies of a seed cell. The oil body is easily separated from other cellular material following lysis of the seed cell, for example by using the partitioning/surface properties of the oil body. The fusion protein may be isolated for example by affinity chromatography using antibodies directed to the oil body protein. Where desired, the polypeptide of interest can be recovered by treatment of the fusion protein with for example a protease capable of recognizing a proteolytic recognition site in the oil body protein proximal to the N-terminus of the polypeptide of interest.
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3.发明公开
公开(公告)号：EP1346056A1
公开(公告)日：2003-09-24
申请号：EP01992353.1
申请日：2001-12-19
发明人： VAN ROOIJEN, Gijs , DECKERS, Harm , HEIFETZ, Peter, Bernard , BRIGGS, Steven, P. , DALMIA, Bipin, Kumar , DEL VAL, Gregg , ZAPLACHINSKI, Steve , MOLONEY, Maurice
IPC分类号： C12N15/79
CPC分类号： C12N15/8258 , C12N15/8257
摘要： Improved methods for the production of multimeric-protein-complexes, such as redox proteins and immunoglobins, in association with oil bodies are described. The redox protein is enzymatically active when prepared in association with the oil bodies. Also provided are related nucleic acids, proteins, cells, plants, and compositions.
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公开(公告)号：EP1289549B1
公开(公告)日：2008-12-10
申请号：EP01944823.2
申请日：2001-06-15
CPC分类号： C07K14/415 , A61K39/39 , A61K2039/54 , A61K2039/55566 , A61K2039/55588 , C07K2319/00
摘要： The present invention relates to the use of oil bodies as a vaccine adjuvant and delivery system for administration of vaccines by parenteral, mucosal (oral, nasal, pulmonary) and transdermal routes. In addition, the present invention relates to methods of eliciting an immune response in an animal by administering oil body-antigen complexes to said mammal. Finally, the present invention relates to methods of preparing oil body-antigen complexes.
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公开(公告)号：EP1633876B1
公开(公告)日：2008-08-20
申请号：EP04737835.1
申请日：2004-06-17
CPC分类号： C07K14/62 , C12N15/8257
摘要： Methods for the production of insulin in plants are described. In one embodiment, the present invention provides a method for the expression of insulin in plants comprising: (a) providing a chimeric nucleic acid construct comprising in the 5’ supl; to 3’ direction of transcription as operably linked components: (i) a nucleic acid sequence capable of controlling expression in plant seed cells; and (ii) a nucleic acid sequence encoding an insulin polypeptide; (b) introducing the chimeric nucleic acid construct into a plant cell; and (c) growing the plant cell into a mature plant capable of setting seed wherein the seed expresses insulin.
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公开(公告)号：EP0871749B1
公开(公告)日：2006-08-16
申请号：EP95941561.3
申请日：1995-12-21
发明人： MOLONEY, Maurice
CPC分类号： C12N9/6483 , C07K14/545 , C07K14/61 , C07K14/8139 , C07K14/815 , C07K2319/00 , C12N9/6429 , C12N15/8234 , C12N15/8241 , C12N15/8242 , C12N15/8257 , C12Y304/21005 , C12Y304/23004
摘要： The present invention relates to the use of a class of genes called oil body protein genes that have unique features. The discovery of these features allowed the invention of methods for the production of recombinant proteins wherein a protein of interest can be easily separated from other host cell components. The invention is further exemplified by methods for exploitation of the unique characteristics of the oil body proteins and oil body genes for expression of polypeptides of interest in many organisms, particularly plant seeds. Said polypeptides may include but are not limited to: seed storage proteins, enzymes, bioactive peptides, antibodies and the like. The invention can also be modified to recover recombinant polypeptides fused to oleosins from non-plant host cells. Additionally the invention provides a method of using recombinant proteins associated with seed oil bodies released during seed germination for expression of polypeptides that afford protection to seedlings from pathogens. Finally, the persistent association of oil body proteins with the oil body can be further utilized to develop a biological means to create novel immobilized enzymes useful for bioconversion of substrates.
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公开(公告)号：EP1007554A1
公开(公告)日：2000-06-14
申请号：EP97946991.3
申请日：1997-12-05
CPC分类号： C07H21/00 , B01D15/08 , B01D15/3804 , B01D15/3809 , C01G11/003 , C07K1/22 , C07K14/415 , C07K16/065 , C08B1/00 , C12N9/6429 , C12Y304/21005
摘要： A method for the separation of a target molecule from a mixture is described. The method employs oil bodies and their associated proteins as affinity matrices for the selective, non-covalent binding of desired target molecules. The oil body proteins may be genetically fused to a ligand having specificity for the desired target molecule. Native oil body proteins can also be used in conjunction with an oil body protein specific ligand such as an antibody or an oil body binding protein. The method allows the separation and recovery of the desired target molecules due to the difference in densities between oil bodies and aqueous solutions.
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公开(公告)号：EP2274014A2
公开(公告)日：2011-01-19
申请号：EP09729878.0
申请日：2009-04-10
发明人： GUTH, Jacob , BOOTH, Joseph , BEAZER, Mitch , SHAFER, Kent
CPC分类号： A61K9/0014 , A23D7/0056 , A61K8/14 , A61K8/345 , A61K8/922 , A61K9/107 , A61K47/10 , A61K47/46 , A61K2800/28 , A61Q19/00
摘要： The physical stability of oleosomes is preserved in a composition by introducing a multihydric alcohol and an acid that can reduce the pH of the composition of less than 6. The resultant composition can be useful in the manufacture of cosmetic, food, and pharmaceutical products, among others.
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公开(公告)号：EP1799831A1
公开(公告)日：2007-06-27
申请号：EP05791719.7
申请日：2005-10-06
CPC分类号： A23D9/00 , C12N15/8247 , C12N15/8251
摘要： Methods to modulate oleosin expression levels in plants are provided. Specifically, methods for preparing seed derived products from seed, in which the composition of seed storage reserves, notably the seed lipid and protein contents, have been altered. In particular the present invention provides methods for preparing seed derived products from seed, in which the seed reserves have been altered by modulation of oleosin gene expression and more particularly the suppression of oleosin gene expression.
摘要翻译： 提供了调节植物中油质蛋白表达水平的方法。 具体而言，用于从种子制备种子衍生产品的方法，其中种子储藏组合物，特别是种子脂质和蛋白质含量已被改变。 具体而言，本发明提供了用于从种子制备种子衍生产品的方法，其中通过调节油质蛋白基因表达并且更具体地抑制油质蛋白基因表达来改变种子储备。
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公开(公告)号：EP1212438B8
公开(公告)日：2007-02-21
申请号：EP00954241.6
申请日：2000-08-25
CPC分类号： C07K14/415 , C12N15/8234
摘要： Novel methods for the expression of non-native genes in flax seeds and the seeds of other plant species are provided. The methods involve the use of seed-specific promoters obtained from flax. Additionally provided are novel flax seed-specific promoters, chimeric nucleic acid constructs comprising novel flax seed-specific promoters, transgenic plant cells, transgenic plants and transgenic plant seeds containing novel flax seed-specific promoters. The promoters and methods are useful, for example, for altering the seed oil and protein composition in flax seed or other plant seeds.
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