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公开(公告)号：EP1633876B1
公开(公告)日：2008-08-20
申请号：EP04737835.1
申请日：2004-06-17
CPC分类号： C07K14/62 , C12N15/8257
摘要： Methods for the production of insulin in plants are described. In one embodiment, the present invention provides a method for the expression of insulin in plants comprising: (a) providing a chimeric nucleic acid construct comprising in the 5’ supl; to 3’ direction of transcription as operably linked components: (i) a nucleic acid sequence capable of controlling expression in plant seed cells; and (ii) a nucleic acid sequence encoding an insulin polypeptide; (b) introducing the chimeric nucleic acid construct into a plant cell; and (c) growing the plant cell into a mature plant capable of setting seed wherein the seed expresses insulin.
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公开(公告)号：EP1007554A1
公开(公告)日：2000-06-14
申请号：EP97946991.3
申请日：1997-12-05
CPC分类号： C07H21/00 , B01D15/08 , B01D15/3804 , B01D15/3809 , C01G11/003 , C07K1/22 , C07K14/415 , C07K16/065 , C08B1/00 , C12N9/6429 , C12Y304/21005
摘要： A method for the separation of a target molecule from a mixture is described. The method employs oil bodies and their associated proteins as affinity matrices for the selective, non-covalent binding of desired target molecules. The oil body proteins may be genetically fused to a ligand having specificity for the desired target molecule. Native oil body proteins can also be used in conjunction with an oil body protein specific ligand such as an antibody or an oil body binding protein. The method allows the separation and recovery of the desired target molecules due to the difference in densities between oil bodies and aqueous solutions.
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公开(公告)号：EP1670434A1
公开(公告)日：2006-06-21
申请号：EP04789702.0
申请日：2004-10-04
CPC分类号： A61Q19/00 , A61K8/06 , A61K9/107 , A61K31/24 , A61K31/685 , A61K36/286
摘要： The present invention provides novel emulsions that comprise oil bodies. The invention also relates to novel methods for generating formulations comprising oil bodies and active ingredients wherein the active ingredient is partitioned into the oil body. The methods are particularly useful for generating emulsions with either hydrophobic or amphipathic biologically active agents.
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公开(公告)号：EP1216306A1
公开(公告)日：2002-06-26
申请号：EP00954228.3
申请日：2000-08-23
CPC分类号： C12N9/6483 , C12N15/8257 , C12Y304/23004
摘要： A method for the commercial production of chymosin which involves the recombinant expression of chymosin in plant seeds, that enables chymosin to accumulate to at least 0,5 % (w/w) of total seed protein is described. An improved method for the isolation of chymosin from the seed is also described.
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公开(公告)号：EP0986309B1
公开(公告)日：2008-02-20
申请号：EP98922563.6
申请日：1998-05-27
发明人： DECKERS, Harm, M. , van ROOIJEN, Gijs , BOOTHE, Joseph , GOLL, Janis , MOLONEY, Maurice , MAHMOUD, Soheil, Sayed
IPC分类号： A23D7/00 , C11B1/10 , B01F17/00 , A23L1/035 , A23K1/16 , A23L1/24 , A23L1/39 , A61K8/00 , A61K9/107
CPC分类号： A23G3/48 , A23D7/001 , A23D7/003 , A23G3/343 , A23G3/346 , A23G3/40 , A23G4/066 , A23G4/068 , A23G9/327 , A23G9/42 , A23G2200/08 , A23G2200/14 , A23K20/168 , A23K20/184 , A23K50/80 , A23L9/10 , A23L23/00 , A23L27/18 , A23L27/60 , A23P20/10 , A61K8/06 , A61K8/922 , A61K9/0014 , A61K9/107 , A61K36/00 , A61K36/185 , A61K36/23 , A61K36/28 , A61K36/286 , A61K36/31 , A61K36/42 , A61K36/47 , A61K36/48 , A61K36/889 , A61K36/899 , A61K47/44 , A61Q1/02 , A61Q1/06 , A61Q1/08 , A61Q1/10 , A61Q5/00 , A61Q11/00 , A61Q13/00 , A61Q17/04 , A61Q19/00 , A61Q19/10 , C09D5/022 , C11B1/10 , Y02A40/818 , Y10S426/805 , Y10S514/937 , A61K2300/00
摘要： The present invention provides novel emulsion formulations which comprise oil bodies. The invention also provides a method for preparing the emulsions and the use of the emulsions in various domestic and industrial compositions. The emulsions are especially suited for the preparation of food products, personal care products, pharmaceutical products and industrial products.
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公开(公告)号：EP1007554B1
公开(公告)日：2006-03-29
申请号：EP97946991.3
申请日：1997-12-05
CPC分类号： C07H21/00 , B01D15/08 , B01D15/3804 , B01D15/3809 , C01G11/003 , C07K1/22 , C07K14/415 , C07K16/065 , C08B1/00 , C12N9/6429 , C12Y304/21005
摘要： A method for the separation of a target molecule from a mixture is described. The method employs oil bodies and their associated proteins as affinity matrices for the selective, non-covalent binding of desired target molecules. The oil body proteins may be genetically fused to a ligand having specificity for the desired target molecule. Native oil body proteins can also be used in conjunction with an oil body protein specific ligand such as an antibody or an oil body binding protein. The method allows the separation and recovery of the desired target molecules due to the difference in densities between oil bodies and aqueous solutions.
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公开(公告)号：EP0986309A1
公开(公告)日：2000-03-22
申请号：EP98922563.6
申请日：1998-05-27
发明人： DECKERS, Harm, M. , van ROOIJEN, Gijs , BOOTHE, Joseph , GOLL, Janis , MOLONEY, Maurice , MAHMOUD, Soheil, Sayed
IPC分类号： A23D7/00 , C11B1/10 , B01F17/00 , A23L1/035 , A23K1/16 , A23L1/24 , A23L1/39 , A61K7/00 , A61K9/107
CPC分类号： A23G3/48 , A23D7/001 , A23D7/003 , A23G3/343 , A23G3/346 , A23G3/40 , A23G4/066 , A23G4/068 , A23G9/327 , A23G9/42 , A23G2200/08 , A23G2200/14 , A23K20/168 , A23K20/184 , A23K50/80 , A23L9/10 , A23L23/00 , A23L27/18 , A23L27/60 , A23P20/10 , A61K8/06 , A61K8/922 , A61K9/0014 , A61K9/107 , A61K36/00 , A61K36/185 , A61K36/23 , A61K36/28 , A61K36/286 , A61K36/31 , A61K36/42 , A61K36/47 , A61K36/48 , A61K36/889 , A61K36/899 , A61K47/44 , A61Q1/02 , A61Q1/06 , A61Q1/08 , A61Q1/10 , A61Q5/00 , A61Q11/00 , A61Q13/00 , A61Q17/04 , A61Q19/00 , A61Q19/10 , C09D5/022 , C11B1/10 , Y02A40/818 , Y10S426/805 , Y10S514/937 , A61K2300/00
摘要： The present invention provides novel emulsion formulations which comprise oil bodies. The invention also provides a method for preparing the emulsions and the use of the emulsions in various domestic and industrial compositions. The emulsions are especially suited for the preparation of food products, personal care products, pharmaceutical products and industrial products.
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公开(公告)号：EP1216306B1
公开(公告)日：2008-07-09
申请号：EP00954228.3
申请日：2000-08-23
CPC分类号： C12N9/6483 , C12N15/8257 , C12Y304/23004
摘要： A method for the commercial production of chymosin which involves the recombinant expression of chymosin in plant seeds, that enables chymosin to accumulate to at least 0,5 % (w/w) of total seed protein is described. An improved method for the isolation of chymosin from the seed is also described.
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公开(公告)号：EP1633876A2
公开(公告)日：2006-03-15
申请号：EP04737835.1
申请日：2004-06-17
CPC分类号： C07K14/62 , C12N15/8257
摘要： Methods for the production of insulin in plants are described. In one embodiment, the present invention provides a method for the expression of insulin in plants comprising: (a) providing a chimeric nucleic acid construct comprising in the 5’ supl; to 3’ direction of transcription as operably linked components: (i) a nucleic acid sequence capable of controlling expression in plant seed cells; and (ii) a nucleic acid sequence encoding an insulin polypeptide; (b) introducing the chimeric nucleic acid construct into a plant cell; and (c) growing the plant cell into a mature plant capable of setting seed wherein the seed expresses insulin.
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公开(公告)号：EP1131047A1
公开(公告)日：2001-09-12
申请号：EP99972527.8
申请日：1999-11-24
CPC分类号： A61K8/368 , A61K8/14 , A61K8/347 , A61K8/365 , A61K8/64 , A61K8/67 , A61K8/671 , A61K8/922 , A61K9/0014 , A61K47/44 , A61K2800/28 , A61Q17/04 , A61Q19/02 , A61Q19/08
摘要： The present invention relates to the topical delivery of active agents, for example cosmetic compounds, to living organisms. In particular the present invention relates to the administration of therapeutically active agents in the presence of compositions obtainable from living cells known as oil bodies. The oil bodies of the present invention are especially suitable in applications where it is desired that the active agent is topically delivered. The oil bodies of the present invention are preferably obtained from oil seed plants.
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