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182 条记录 (耗时 0.038 秒)

    Method for protection against genotoxic mutagenesis
    5.
    发明公开
    Method for protection against genotoxic mutagenesis 失效
    方法以防止基因毒性诱变

    公开(公告)号：EP1437139A3

    公开(公告)日：2004-09-29

    申请号：EP04004524.7

    申请日：1994-09-12

    申请人： ARCH DEVELOPMENT CORPORATION

    发明人： Grdina, David

    IPC分类号： A61K31/661

    CPC分类号： C07F9/1651 ,  A61K31/13 ,  A61K31/185 ,  A61K31/425 ,  A61K31/66 ,  A61K31/661 ,  A61K45/06 ,  C07C323/25 ,  C07H5/10 ,  C07H9/06 ,  A61K2300/00

    摘要： A method for protecting against mutational damage in mammalian cells induced by irradiation comprising administering a phosphorothioate or phosphorothiate metabolite selected from the group consisting S-2-(3-aminopropylamino)ethylphosphorothioic acid, S-1-(aminoethyl) phosphorothioic acid, S-[2-(3-methylaminopropyl) aminoethyl] phosphorothioate acid, S-2-(4-aminobu-lamino) ethylphosphorothioic acid, 3-[(2-mercaptoethyl) amino] propionamide p-toluenesulfonate, S-1-(2-hydroxy-3-amino) propyl phosphorothioic acid, 2-[3-(methylamino) propylamino] ethanethiol, 2-(aminopropylamino)ethanethiol, S-2-(5-aminopentylamino) ethyl phosphorothioic acid, 1 [3-(3-(aminopropyl) thiazolidin-2-Y1]-D-gluco-1,2,3,4,5 pentanepentol dihydrochloride and N,N'-(dithiodi-2,1-ethanediyl)bis-1,3-propanediamme to the mammal before or up to 3 hours after irradiation.

    Interaction trap system for isolating proteins
    8.
    发明公开
    Interaction trap system for isolating proteins 失效
    用于分离这些蛋白交互系统的情况下

    公开(公告)号：EP1362913A3

    公开(公告)日：2003-11-26

    申请号：EP03017549.1

    申请日：1993-10-20

    申请人： THE GENERAL HOSPITAL CORPORATION

    发明人： The designation of the inventor has not yet been filed

    IPC分类号： C12N15/10 ,  C12Q1/68

    CPC分类号： C12Q1/6897 ,  A61K38/00 ,  C07K14/4738 ,  C07K2319/00 ,  C07K2319/61 ,  C07K2319/71 ,  C07K2319/80 ,  C12N9/1205 ,  C12N9/16 ,  C12N15/1055 ,  C12Q2565/201

    摘要： Disclosed is a method for determining whether a first proteins capable of physically interacting with a second protein. The method involves: (a) providing a host cell which contains (i) a reporter gene operably linked to a protein binding site; (ii) a first fusion gene which expresses a first fusion protein, the first fusion protein including the first protein covalently bonded to a binding moiety which is capable of specifically binding to the protein binding site; and (iii) a second fusion gene which expresses a second fusions protein, the second fusion protein including the second protein covalently bonded t a weak gene activating moiety; and (b) measuring expression of the reporter gene as a measure of an interaction between the first and the second proteins. Such a determination facilitated the isolation of the gene encoding the interacting protein. Also disclosed herein is recombinant Cdil polypeptide, nucleic acid encoding the Cdil polypeptide, and uses thereof.

    INTERACTION TRAP SYSTEMS FOR DETECTING PROTEIN INTERACTIONS
    9.
    发明授权
    INTERACTION TRAP SYSTEMS FOR DETECTING PROTEIN INTERACTIONS 失效
    互动FULL系统在蛋白质相互作用的检测

    公开(公告)号：EP0773952B1

    公开(公告)日：2003-11-12

    申请号：EP95928118.9

    申请日：1995-07-20

    申请人： THE GENERAL HOSPITAL CORPORATION ,  Genetics Institute, LLC

    发明人： BRENT, Roger ,  MCCOY, John, M. ,  JESSEN, Timm, H. ,  XU, Chanxing, Wilson

    IPC分类号： C07H21/04 ,  C07K19/00 ,  C12N1/19 ,  C12N5/10 ,  C12Q1/68 ,  G01N33/68

    CPC分类号： C12N9/0036 ,  C07K2319/00 ,  C07K2319/35 ,  C07K2319/70 ,  C07K2319/71 ,  C07K2319/80 ,  C12N15/1055 ,  C12Q1/6897

    摘要： Disclosed herein is a method of determining whether a first protein is capable of physically interacting with a second protein, involving: (a) providing a host cell which contains (i) a reporter gene operably linked to a protein binding site; (ii) a first fusion gene which expresses a first fusion protein, the first fusion protein including the first protein covalently bonded to a binding moiety which is capable of specifically binding to the protein binding site; and (iii) a second fusion gene which expresses a second fusion protein, the second fusion protein including the second protein covalently bonded to a gene activating moiety and being conformationally-constrained; and (b) measuring expression of the reporter gene as a measure of an interaction between the first and the second proteins. Also disclosed are methods for assaying protein interactions, and identifying antagonists and agonists of protein interactions. Proteins isolated by these methods are also discussed. Finally, populations of eukaryotic cells are disclosed, each cell having a recombinant DNA molecule encoding a conformationally-constrained intracellular peptide.