公开(公告)号：EP1692258A2

公开(公告)日：2006-08-23

申请号：EP04801001.1

申请日：2004-11-12

申请人： Xu, Xiao ,  Abbassi, Yama ,  Wang, Xiaobo ,  Gan, Jiangbo

发明人： Xu, Xiao ,  Abbassi, Yama ,  Wang, Xiaobo ,  Gan, Jiangbo

IPC分类号： C12M1/34 ,  C12M1/42

CPC分类号： G01N33/6854 ,  C12M25/08 ,  C12M41/36 ,  G01N33/4836 ,  G01N33/5005 ,  G01N2500/10

摘要： The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. In some preferred aspects, the assays are designed to investigate the affects of compounds on cells, such as cytotoxicity assays. In other preferred aspects, the assays are designed to investigate the compounds that effect IgE-mediated responses of cells to antigens.

公开(公告)号：EP1749203A2

公开(公告)日：2007-02-07

申请号：EP05722991.6

申请日：2005-02-09

申请人： Xu, Xiao ,  Abbassi, Yama ,  Wang, Xiaobo

发明人： Xu, Xiao ,  Abbassi, Yama ,  Wang, Xiaobo

IPC分类号： G01N27/02 ,  G01N33/487 ,  G01N33/543 ,  C12M1/34

CPC分类号： G01N33/4836 ,  C12M25/08 ,  C12M41/36 ,  G01N27/021 ,  G01N33/5005

摘要： The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. The methods can be used to test the effects of compounds on cells, such as in cytotoxicity assays. Methods of cytotoxicity profiling of compounds are also provided.

公开(公告)号：EP2639576A1

公开(公告)日：2013-09-18

申请号：EP13171137.6

申请日：2005-02-09

申请人： Xu, Xiao ,  Abbassi, Yama ,  Wang, Xiaobo

发明人： Xu, Xiao ,  Abbassi, Yama ,  Wang, Xiaobo

IPC分类号： G01N27/02 ,  G01N33/487 ,  G01N33/543 ,  C12M1/34

CPC分类号： C12M25/08 ,  C12M41/36 ,  G01N27/021 ,  G01N33/4836 ,  G01N33/5005

摘要： The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. The methods can be used to test the effects of compounds on cells, such as in cytotoxicity assays. Methods of cytotoxicity profiling of compounds are also provided.

公开(公告)号：EP2450698A1

公开(公告)日：2012-05-09

申请号：EP11193882.5

申请日：2005-02-09

申请人： Abbassi, Yama ,  Wang, Xiaobo ,  Xu, Xiao

发明人： Abbassi, Yama ,  Wang, Xiaobo ,  Xu, Xiao

IPC分类号： G01N27/02 ,  G01N33/487 ,  G01N33/543 ,  C12M1/34

CPC分类号： C12M25/08 ,  C12M41/36 ,  G01N27/021 ,  G01N33/4836 ,  G01N33/5005

摘要： The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. The methods can be used to test the effects of compounds on cells, such as in cytotoxicity assays. Methods of cytotoxicity profiling of compounds are also provided.

摘要翻译： 本发明包括装置，系统，以及用于使用细胞底物阻抗监测测定细胞的方法。 在一个方面，本发明提供了细胞 - 基底阻抗监测装置上的不导电基板，其中每个阵列具有跨越整个阵列在大致均匀的电极电阻的确包括电极阵列。 在另一个方面，本发明提供了细胞 - 基底的监测系统，其包括一种或多种细胞底物监测装置包括以阻抗分析仪电极阵列，每个具有多个孔，一个设备站没有连接各个孔的阵列以阻抗分析仪和软件 用于控制设备站和阻抗分析器。 在另一个方面，本发明提供细胞试验确实使用了阻抗监测，以检测在细胞行为或状态的变化。 的方法可用于测试化合物对细胞的作用，如在细胞毒性试验检测。 因此，提供的化合物的细胞毒性分析的方法。

公开(公告)号：EP1773979A2

公开(公告)日：2007-04-18

申请号：EP05786773.1

申请日：2005-08-04

申请人： Abassi, Yama A. ,  Yu, Naichen ,  Atienza, Josephine ,  Xu, Xiao ,  Wang, Xiaobo

发明人： Abassi, Yama A. ,  Yu, Naichen ,  Atienza, Josephine ,  Xu, Xiao ,  Wang, Xiaobo

IPC分类号： C12M1/34 ,  H01M4/50

CPC分类号： G01N33/4836

摘要： The present application includes systems and methods for identifying a compound capable of interacting with a G-Protein Coupled Receptor (GPCR) or Receptor Tyrosine Kinase (RTK) including providing a device capable of measuring cell-substrate impedance operably connected to an impedance analyzer, adding test cells expressing a GPCR or a RTK to wells of the device, monitoring the impedances of the wells before and after adding a compound of interest, determining the change in the impedance or cell index of a well, comparing the change in impedance or cell index between the compound and the control, and identifying the compound interacts with the GPCR or RTK if the comparison demonstrates a significant difference between the change in impedance or cell index.

公开(公告)号：EP1815025A2

公开(公告)日：2007-08-08

申请号：EP05852157.6

申请日：2005-11-23

申请人： Abassi, Yama A. ,  Wang, Xiaobo ,  Atienza, Josephine ,  Xu, Xiao ,  Xu, Junquan

发明人： Abassi, Yama A. ,  Wang, Xiaobo ,  Atienza, Josephine ,  Xu, Xiao ,  Xu, Junquan

IPC分类号： C12Q1/68

CPC分类号： G01N33/5008 ,  C12M35/02 ,  C12N15/87 ,  G01N15/12 ,  G01N33/48728

摘要： The present invention includes devices and methods for transfecting a cell or cell population and dynamic monitoring of cellular events. A variety of microelectronic devices are provide that incorporate functions such as electroporation, modulation of a transmembrane potential and dynamic monitoring of cellular functions and mechanisms.

公开(公告)号：EP1773980A2

公开(公告)日：2007-04-18

申请号：EP05804409.0

申请日：2005-08-04

申请人： Xu, Xiao ,  Abassi, Yama A. ,  Wang, Xiaobo

发明人： Xu, Xiao ,  Abassi, Yama A. ,  Wang, Xiaobo

IPC分类号： C12M1/34 ,  H01M4/50

CPC分类号： G01N33/4836

摘要： The present invention includes a method of measuring cytolytic activity including providing a device capable of monitoring cell-substrate impedance operably connected to an impedance analyzer, adding target cells to at least one well of the device, adding effector cells to the at least one well, monitoring impedance of the at least one well and optionally determining a cell index from the impedance, wherein monitoring impedance includes measuring impedance during at least one time point before and at least one time point after adding effector cells, and determining viability of said target cells after adding effector cells by comparing the impedance or optionally the cell index at the at least one time point after adding effector cells to the impedance or optionally the cell index at the at least one time point before adding effector cells.

公开(公告)号：EP1800312A2

公开(公告)日：2007-06-27

申请号：EP05812268.0

申请日：2005-09-27

申请人： Abassi, Yama A. ,  Atienza, Josephine ,  Xu, Xiao ,  Wang, Xiaobo

发明人： Abassi, Yama A. ,  Atienza, Josephine ,  Xu, Xiao ,  Wang, Xiaobo

IPC分类号： G11C11/00 ,  C12M1/34 ,  H01M4/50

CPC分类号： G01N29/036 ,  G01N2291/0257

摘要： The present invention includes devices and methods for dynamically monitoring cell adhesion and cell spreading. Cells are added to a microelectronic cell sensor array operably connected to an impedance analyzer. The device also includes a coating including biological molecule or organic compound capable of interacting with the cell. Cell adhesion and cell mobility is determined by detecting changes in impedance and comparing impedance or cell index values between samples.