公开(公告)号：EP1689764A4

公开(公告)日：2010-08-18

申请号：EP04811635

申请日：2004-11-19

申请人： ALLELOGIC BIOSCIENCES CORP

发明人： MAO FEI ,  XIN XING

IPC分类号： C07H21/02 ,  C07H20060101 ,  C07H21/04 ,  C12Q1/68

CPC分类号： C12Q1/6818 ,  C12Q1/6851 ,  Y10S435/975 ,  C12Q2565/1015 ,  C12Q2561/101 ,  C12Q2565/1025

公开(公告)号：EP1689764A2

公开(公告)日：2006-08-16

申请号：EP04811635.4

申请日：2004-11-19

申请人： Allelogic Biosciences Corp.

发明人： MAO, Fei ,  XIN, Xing

IPC分类号： C07H1/00

CPC分类号： C12Q1/6818 ,  C12Q1/6851 ,  Y10S435/975 ,  C12Q2565/1015 ,  C12Q2561/101 ,  C12Q2565/1025

摘要： An embodiment of the invention discloses new methods for designing labeled nucleic acid probes and primers by labeling oligonucleotides with a plurality of spectrally identical or similar dyes and optionally with one or more quencher dyes. Oligonucleotides labeled in accordance with some embodiments of the invention exhibit a detectable increase in signal, for example, fluorescent signal when the labeling dyes are separated from one another. Methods for separating the dye include cleaving the labeled oligonucleotides include using enzymes that have 5'-exonuclease activity. In one embodiment nucleic acid primers of the present invention may fluoresce upon hybridization to a target sequence and incorporation into the amplification product. Nucleic acid probes and primers of the present invention have wide applications ranging from general detection of a target nucleic acid sequence to clinical diagnostics. Major advantages of the oligonucleotides including nucleic acid probes and primers of many embodiments of the present invention are their synthetic simplicity, spectral versatility and superior fluorescent signal.

公开(公告)号：EP2307558A4

公开(公告)日：2012-08-08

申请号：EP09773928

申请日：2009-07-02

申请人： ALLELOGIC BIOSCIENCES CORP

发明人： MAO FEI ,  XIN XING ,  LEUNG WAI-YEE

IPC分类号： C12P19/34 ,  C08H1/00 ,  C12N9/99

CPC分类号： C12Q1/6844 ,  C07K1/064 ,  C08H1/00 ,  C12N9/1252 ,  C12N9/127 ,  C12P19/34 ,  C12Q2521/101 ,  C12Q2521/119

公开(公告)号：EP1863933A4

公开(公告)日：2009-04-08

申请号：EP06738900

申请日：2006-03-17

申请人： BIOTIUM INC ,  ALLELOGIC BIOSCIENCES CORP

发明人： MAO FEI ,  LEUNG WAI-YEE ,  XIN XING

IPC分类号： C12Q1/68 ,  C07D213/02 ,  C07D401/02

CPC分类号： C12Q1/6876 ,  C07D209/08 ,  C07D213/38 ,  C07D221/10 ,  C07D417/06 ,  C07D417/14 ,  C07D491/147 ,  C07H21/00 ,  C09B15/00 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q2563/173

摘要： Dimeric and trimeric nucleic acid dyes, and associated systems and methods are provided. Such a dye may form a hairpin-like structure that enables it to stain nucleic acids via a release-on-demand mechanism, for example. Such a dye may have low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, for example. A dye provided herein may be useful in a variety of applications, such as in DNA quantitation in real-time PCR, for example.