公开(公告)号：EP0839914A1

公开(公告)日：1998-05-06

申请号：EP97919404.0

申请日：1997-04-18

申请人： Antibioticos, S.A.

发明人： GARCIA LOPEZ, José Luis ,  CORTES RUBIO, Estrella ,  GUISAN SEIJAS, José Manuel ,  BARREDO FUENTE, José Luis ,  DIEZ GARCIA, Bruno ,  COLLADOS DE LA VIEJA, Alfonso ,  VITALLER ALBA, Alejandro ,  SALTO MALDONADO, Francisco

IPC分类号： C12N15/55 ,  C12N9/14 ,  C12N1/21 ,  C12P35/06 ,  C07K1/22

CPC分类号： C12N9/86 ,  C07K2319/00 ,  C12N9/84

摘要： A procedure for producing a modified 7β-(4-carboxybutanamide) cephalosporinase enzyme which can be purified in a single chromatographic step. The procedure for production of the enzyme involves: mutagenizing the gene which codes for the enzyme from Acinetobacter sp . ATCC 53891 by inserting a nucleotide sequence coding for six histidine residues; fusing the mutant gene with high-efficiency promoter DNA sequences; transforming Escherichia coli cells with the fusion gene construct; growing the transformed Escherichia coli cells; and recovering the enzyme by the use of supports which contain metal chelates. 7-Aminocephalosporanic acid is an important intermediate for the manufacture of a wide range of antibacterial agents of the cephalosporin family.

摘要翻译： 一种生产改性7测试程序 - （4-羧丁基酰胺）头孢菌素酶，其可以在单个层析步骤中纯化。 产生酶的过程包括：诱变基因，其编码来自不动杆菌的酶。 ATCC 53891通过插入编码六个组氨酸残基的核苷酸序列; 熔融配合高效率启动子的DNA序列的突变基因; 转化大肠杆菌细胞与所述融合基因构建体; 培育转化的大肠杆菌细胞; 和回收通过使用含有金属螯合物的载体的酶。 7-氨基头孢烷酸是适用范围广的头孢菌素家族的抗菌剂的制造中的重要中间体。

公开(公告)号：EP0209363A3

公开(公告)日：1988-03-09

申请号：EP86305447

申请日：1986-07-15

申请人： Antibioticos, S.A.

发明人： Luengo, Jose Maria c/o Antibioticos S.A. ,  Lopez-Nieto, M. J. c/o Antibioticos S.A. ,  Salto, Francisco c/o Antibioticos S.A.

IPC分类号： C07K05/06 ,  C12P37/00 ,  C12R01/75 ,  C12R01/82 ,  C12R01/465

CPC分类号： C07K5/0606 ,  C12P37/00 ,  C12R1/465 ,  C12R1/75 ,  C12R1/82

摘要： Penicillin G and analogous penicillins with arylacylamido side chains can be produced by the action of cyclase on PCV, POCV, or appropriate analogue thereof.

公开(公告)号：EP0209363A2

公开(公告)日：1987-01-21

申请号：EP86305447.4

申请日：1986-07-15

申请人： Antibioticos, S.A.

发明人： Luengo, Jose Maria c/o Antibioticos S.A. ,  Lopez-Nieto, M. J. c/o Antibioticos S.A. ,  Salto, Francisco c/o Antibioticos S.A.

IPC分类号： C07K5/06 ,  C12P37/00

CPC分类号： C07K5/0606 ,  C12P37/00 ,  C12R1/465 ,  C12R1/75 ,  C12R1/82

摘要： Penicillin G and analogous penicillins with arylacylamido side chains can be produced by the action of cyclase on PCV, POCV, or appropriate analogue thereof.

摘要翻译： 青霉素G和类似的具有芳基酰氨基酰氨基侧链的青霉素可以通过环化酶对PCV，POCV或其合适的类似物的作用产生。

公开(公告)号：EP0167651A1

公开(公告)日：1986-01-15

申请号：EP84108148.2

申请日：1984-07-11

申请人： Antibioticos, S.A.

发明人： Lizarbe, D. Jose Ramon Fernandez, Dr. ,  Alba, D. Alejandro Vitaller, Dr.

IPC分类号： C07D501/18 ,  C07D501/12 ,  C12P35/00 ,  C07D501/36

CPC分类号： C12P35/00

摘要： The subject matter of the invention is a process for producing 3-cephem-4-carboxylic acid derivatives substituted in 3-position of formula (1)

wherein R 2 is thienyl, diazolyl, thiazolyl, tetrazolyl, thiadiazolyl, thiatriazolyl, oxazolyl, oxadiazolyl, pyridyl, pyrimidinyl, benzothiazolyl, benzimidazolyl, benzoxazolyl or any of their substituted derivatives in any of the possible positions of the mentioned heterocyclic residues available for substitution, which is characterized in that a 7-aminocephalosporanic acid derivative of formula (II)
wherein R 1 means -OOCCH 2 is reacted with a thiol R 2 SH. The compounds I may be effectively purified by adsorption on a strongly basic anion exchange resin.
The compounds 1 which are obtained by the process of the invention are valuable intermediates in the synthesis of therapeutically useful cephalosporins.

摘要翻译： 本发明的主题是制备在式（I）的3-位取代的3-头孢烯-4-羧酸衍生物的方法，其中R 2是噻吩基，二唑基，噻唑基，四唑基，噻二唑基，三唑基，恶唑基， 恶二唑基，吡啶基，嘧啶基，苯并噻唑基，苯并咪唑基，苯并恶唑基或任何可取代的杂环残基的任何可能取代的衍生物，其特征在于式（II）的7-氨基 - 头孢烷酸衍生物 其中R1表示-OOCCH2与硫醇R2SH反应。 化合物I可以通过在强碱性阴离子交换树脂上的吸附而被有效地纯化。 通过本发明的方法获得的化合物I是合成治疗有用的头孢菌素的有价值的中间体。

公开(公告)号：EP1472259B1

公开(公告)日：2009-09-02

申请号：EP03734703.6

申请日：2003-01-28

申请人： CIPAN-Companhia Industrial Produtora De Antibioticos, S.A.

发明人： ROCHA GONCALVES, Antonio ,  PINEIRO, Marta ,  SERRA, Armenio, C.

IPC分类号： C07D487/22 ,  A61K31/409 ,  A61K49/00 ,  A61P35/00 ,  C07D257/00

CPC分类号： C07D487/22 ,  A61K41/0071

摘要： The invention refers to macrocyclic tetrapirrolic compounds of the family of porphyrins (structure I), chlorins (structure II) and bacteriochlorins (structure III) characterized by the presence of hydrogen or halogen atoms and/or phenyl groups with halogens in the ortho position (with or without hydroxyl groups in the meta position) in positions 5,10,15 and 20 of the macrocycle, and hydrogen or halogen atoms or akyl, vinyl, carboxy, carboxyalkyl, alkoxycarbonyl, alkoxycetoalkyl, alcoxycetoaryl, acetoxy or hydroxyalkyl groups in positions 2,3,7,8,12,13,17 and/or 18.

公开(公告)号：EP1460060A1

公开(公告)日：2004-09-22

申请号：EP02796800.7

申请日：2002-12-20

申请人： Antibioticos, S.A.

发明人： ESTRELLA DE CASTRO, Antonio ,  FRAILE YECORA, Nieves ,  OLIVER RUIZ, Manuel ,  MUNOZ, Angel ,  LOPEZ ORTIZ, Juan Francisco ,  CABRI, Walter

IPC分类号： C07C403/02

CPC分类号： C07C403/24

摘要： A method of obtaining new formulations based on lutein.
The present invention describes a method of preparation of formulations of microcrystalline lutein, particularly in the form of esters, which are resistant to oxidation and are soluble in hydrophilic and/or lipophilic media. For these formulations, the esters of lutein are mixed with antioxidants, vegetable oils and/or organic solvents, and this initial mixture is submitted to various stages depending on the type of final formulation required. These formulations are suitable for direct application as colourants in the pharmaceutical, food and cosmetics fields. They can also be used as diet supplements.

摘要翻译： 获得基于叶黄素的新制剂的方法。 本发明描述了一种制备微晶叶黄素制剂的方法，特别是以酯的形式，它们具有抗氧化作用并且可溶于亲水和/或亲脂性介质。 对于这些制剂，叶黄素的酯与抗氧化剂，植物油和/或有机溶剂混合，并且根据所需的最终制剂的类型将该初始混合物进行各种阶段。 这些制剂适合直接用作制药，食品和化妆品领域的着色剂。 它们也可以用作饮食补充剂。

公开(公告)号：EP0811689B1

公开(公告)日：2002-06-12

申请号：EP96938223.3

申请日：1996-11-15

申请人： Antibioticos, S.A.

发明人： RODRIGUEZ OTERO, Carmelita ,  MORENO VALLE, Miguel Angel ,  LOPEZ NIETO, Manuel Jesús ,  COLLADOS DE LA VIEJA, Alfonso Juan ,  VITALLER ALBA, Alejandro

IPC分类号： C12P17/18

CPC分类号： C12P17/188 ,  Y10S435/886

摘要： A batch fermentation is carried out continuously or semicontinuously using strains of Streptomyces clavuligerus ATCC 27064 or mutants thereof. The fermentation process is carried out with a strict control of the soluble phosphate in the medium, both at the beginning and throughout the process. As a carbon source, lipids, preferably triglycerides can be used. Substantial increases in the production of the antibiotic are obtained.

公开(公告)号：EP0843015A1

公开(公告)日：1998-05-20

申请号：EP97919405.7

申请日：1997-04-18

申请人： Antibioticos, S.A.

发明人： GARCIA LOPEZ, José Luis ,  CORTES RUBIO, Estrella ,  ALONSO PALACIOS, Jorge ,  BARREDO FUENTE, José Luis ,  DIEZ GARCIA, Bruno ,  MORENO VALLE, Miguel Angel ,  SCHLEISSNER SANCHEZ, Carmen ,  COLLADOS DE LA VIEJA, Alfonso ,  VITALLER ALBA, Alejandro ,  SALTO MALDONADO, Francisco

IPC分类号： C12N15/53 ,  C12N9/06 ,  C12P35/06 ,  C12N1/21

CPC分类号： C12N9/0024

摘要： Process for producing the enzyme D-amino acid oxydase of Rhodotorula gracilis in host cells. The process for the expression of the enzyme comprises isolating the complementary DNA corresponding to the messager RNA of the gene which codes for the D-amino acid oxydase of any strain of Rhodotorula gracillis producing said enzyme; fusing the fragment of DNA which codes for D-amino acid oxydase of Rhodotorula gracillis with a DNA sequence which contains a site of union to the ribosome and a high efficiency promoter sequence for the express of genes in host cells; inserting the DNA fragment into a plasmid appropriate for the host cell; cultivating the host cells transformed with said plasmid; and collecting the enzyme.

摘要翻译： 在宿主细胞中生产细小红球菌的D-氨基酸氧化酶酶的方法。 表达酶的过程包括分离对应于编码产生所述酶的任何红曲霉菌株的D-氨基酸氧化酶的基因的信使RNA的互补DNA; 将编码红景天细菌的D-氨基酸氧化酶的DNA片段与含有与核糖体结合的位点的DNA序列和用于在宿主细胞中表达基因的高效启动子序列融合; 将DNA片段插入适合宿主细胞的质粒中; 培养用所述质粒转化的宿主细胞; 并收集酶。

公开(公告)号：EP0811689A1

公开(公告)日：1997-12-10

申请号：EP96938223.3

申请日：1996-11-15

申请人： Antibioticos, S.A.

发明人： RODRIGUEZ OTERO, Carmelita ,  MORENO VALLE, Miguel Angel ,  LOPEZ NIETO, Manuel Jesús ,  COLLADOS DE LA VIEJA, Alfonso Juan ,  VITALLER ALBA, Alejandro

IPC分类号： C12P17/18

CPC分类号： C12P17/188 ,  Y10S435/886

摘要： A batch fermentation is carried out continuously or semicontinuously using strains of Streptomyces clavuligerus ATCC 27064 or mutants thereof. The fermentation process is carried out with a strict control of the soluble phosphate in the medium, both at the beginning and throughout the process. As a carbon source, lipids, preferably triglycerides can be used. Substantial increases in the production of the antibiotic are obtained.

摘要翻译： 使用Streptomyces clavuligerus ATCC 27064或其突变体的菌株连续或半连续地进行分批发酵。 在开始和整个过程中，对培养基中可溶性磷酸盐的严格控制进行发酵过程。 作为碳源，可以使用脂质，优选甘油三酸酯。 获得抗生素生产的实质性增加。

公开(公告)号：EP0209363B1

公开(公告)日：1992-05-06

申请号：EP86305447.4

申请日：1986-07-15

申请人： Antibioticos, S.A.

发明人： Luengo, Jose Maria c/o Antibioticos S.A. ,  Lopez-Nieto, M. J. c/o Antibioticos S.A. ,  Salto, Francisco c/o Antibioticos S.A.

IPC分类号： C07K5/06 ,  C12P37/00

CPC分类号： C07K5/0606 ,  C12P37/00 ,  C12R1/465 ,  C12R1/75 ,  C12R1/82

摘要： Penicillin G and analogous penicillins with arylacylamido side chains can be produced by the action of cyclase on PCV, POCV, or appropriate analogue thereof.