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    Gene coding for a polypeptide which enhances virus infection of host insects
    2.
    发明公开
    Gene coding for a polypeptide which enhances virus infection of host insects 失效
    编码这增加寄主昆虫的病毒感染的多肽的基因。

    公开(公告)号：EP0596508A1

    公开(公告)日：1994-05-11

    申请号：EP93117929.5

    申请日：1993-11-04

    申请人： BOYCE THOMPSON INSTITUTE FOR PLANT RESEARCH, INC.

    发明人： Granados, Robert R. ,  Hashimoto, Yoshifumi, Dept.of Applied Biology

    IPC分类号： C07K15/00 ,  C12N15/34 ,  A01N63/00

    CPC分类号： C07K14/005 ,  A01N63/02 ,  C12N15/86 ,  C12N2710/14121 ,  C12N2710/14122 ,  C12N2710/14143 ,  Y10S530/826

    摘要： Isolated and cloned baculovirus genes encoding a polypeptide protein present in the occlusion body of certain baculoviruses such as Trichoplusia ni granulosis virus and Pseudaletia unipuncta granulosis virus Hawaiian strain, said genes possessing a biological activity of enhancing virus infection of host insects by causing rapid degradation of the peritrophic membrane lining the midgut lumen of insects. The invention is also directed to pesticides incorporating said genes, i.e. the purified conserved and enhancing baculovirus proteins herein termed enhancins.

    摘要翻译： 编码本中的某些杆状病毒的闭塞体的多肽的蛋白质分离的和克隆的杆状病毒基因：如粉纹夜蛾颗粒体病毒和粘虫unipuncta颗粒体病毒夏威夷菌株，所述基因波塞通过使迅速降解唱寄主昆虫的增强病毒感染的生物活性 食膜衬昆虫中肠流明。 因此，本发明涉及杀虫剂掺入所述基因，即 将纯化的保守的，并且在称之enhancins提高杆状病毒蛋白。

    DNA SEQUENCE CODING FOR A POLYPEPTIDE WHICH ENHANCES VIRUS INFECTION OF HOST INSECTS
    5.
    发明公开
    DNA SEQUENCE CODING FOR A POLYPEPTIDE WHICH ENHANCES VIRUS INFECTION OF HOST INSECTS 失效
    DNA序列编码的宿主昆虫增强多肽的病毒感染

    公开(公告)号：EP0847401A1

    公开(公告)日：1998-06-17

    申请号：EP96932934.0

    申请日：1996-08-23

    申请人： BOYCE THOMPSON INSTITUTE FOR PLANT RESEARCH, INC.

    发明人： GRANADOS, Robert, R.

    IPC分类号： C12N15 ,  A01N63 ,  C07K14 ,  C12N7 ,  A61K38 ,  C12R1

    CPC分类号： C07K14/005 ,  A01N63/02 ,  A61K38/00 ,  C12N2710/14143 ,  C12N2750/12022 ,  Y10S530/825 ,  Y10S530/826

    摘要： This disclosure relates to an isolated and cloned DNA from a granulovirus virus which comprises an amino acid sequence of the viral gene encoding a polypeptide isolated from occlusion bodies of certain baculoviruses and which polypeptide possesses the biological activity of enhancing baculovirus infectivity. Such proteins termed herein as 'enhancins' are found within the viral occlusion body, have a disruptive effect on the insect peritrophic membrane (PM) proteins, and/or interact with the midgut epithelium in such a manner as to permit the increased adsorption, penetration and uptake of virus particles by midgut cells with a concomitant increase in host mortality. Disclosed herein is a recombinant DNA sequence which codes for the enhancin protein of the Helicoverpa armigera granulovirus virus. The DNA sequence is shown in SEQ. ID. NO. :1 and the open reading frame is shown in SEQ. ID. NO.: 1: base pairs 271-2976. The amino acid sequence of the enhancin protein is shown in SEQ. ID. NO.: 2.

    GENETIC ENGINEERING IN PROCARYOTIC ORGANISM
    7.
    发明公开
    GENETIC ENGINEERING IN PROCARYOTIC ORGANISM 失效
    遗传转化原核生物。

    公开(公告)号：EP0113781A1

    公开(公告)日：1984-07-25

    申请号：EP83902654.0

    申请日：1983-07-06

    申请人： BOYCE THOMPSON INSTITUTE FOR PLANT RESEARCH, INC.

    发明人： SZALAY, Aladar, A. ,  WILLIAMS, John, G., K.

    IPC分类号： C12N15 ,  C12N1

    CPC分类号： C12N15/90 ,  C12N15/63

    摘要： Micro-organisme procaryotique et son procédé de production, le micro-organisme contenant au moins une partie d'ADN stable dans le chromosome. Les micro-organismes ci-décrits ainsi que leurs descendants sont exempts de restructuration génétique comprenant l'ADN étranger. Dans un mode préférentiel de réalisation on utilise des cyanobactéries. Les micro-organismes sont produits en introduisant dans la cellule un véhicule d'insertion contenant de l'ADN étranger lié entre deux parties d'ADN homologues à des parties adjacentes du chromosome du récepteur.