摘要:
The invention relates to a method for automated in situ determination of an analyte content of a liquid sample by means of a bioanalyzer, which has a microfluidic device having at least one measuring channel and means for conducting one or more liquids through the measuring channel, wherein the measuring channel comprises at least one substrate, said method having the step sequence for performing a measurement at least within a measurement region in a repeatable manner: (i) preparing a sensor matrix comprising a plurality of receptors binding the analyte and/or a further target molecule, preferably selectively and specifically, particularly due to an affinitive interaction, or bringing about a chemical conversion of the analyte or the further target molecule, having a step of conducting a preparation solution of at least one first chemical species having at least one functional group binding to the substrate and at least one further functional group through the measuring channel, wherein a plurality of the first chemical species is bonded to the substrate by means of the functional group bonding to the substrate, wherein the further functional group services as a receptor or for subsequently bonding to a receptor for the plurality of the first chemical species bonded to the substrate; (ii) conducting the liquid sample or a measurement fluid obtained by treating the liquid sample with at least one reagent through the measuring channel, wherein analyte present in the liquid sample or the measurement liquid and/or further target molecules present in the liquid sample or the measurement liquid, preferably selectively and specifically bond to the receptors or are chemically converted by the same, and determining a measurement variable correlating to the quantity of the target molecules bonded or converted by the receptors or to the quantity of analyte bonded or converted by the receptors, and deriving therefrom the analyte content of the liquid sample; (iii) regenerating, particularly exposing, the at least one substrate, wherein the sensor matrix and optionally molecules bonded thereto, particularly analyte molecules, further target molecules, or other molecules are released from the substrate and/or are at least partially disintegrated.
摘要:
Disclosed is a device (1) for producing a biological microarray (2) comprising at least one belt-shaped support (3) with receptors (5) which are immobilized thereupon and can form a specific bond with a defined ligand. Said device (1) is provided with a holder (6) for the support (3) and at least one apparatus (8) which is disposed on said holder and applies the receptors (5) to the support (3) that is embodied as a moisture-proof polymer film. The inventive device is further provided with a light source (12) emitting ultraviolet radiation onto the receptors (5) that are applied to the belt. The microarray (2) is able to seal a space accommodating a sample that is to be analyzed against a semiconductor microchip comprising sensors for detecting a receptor-ligand complex.
摘要:
A transport box comprises an energy accumulator, a plurality of sequenced sensor holders with inductive interfaces to respective memosens sensors, a near field communication interface, a microprocessor for controlling communication with the individual interfaces and the near field communication interface.
摘要:
Ein potentiometrischer Sensor, umfasst eine pH-Elektrode zum Erfassen eines pH-Werts; eine Schnittstelle zur Ausgabe eines von dem pH-Wert abhängigen Signals an eine übergeordnete Einheit, insbesondere einen Transmitter (8); einen digitalen Datenspeicher (6), der mit der pH-Elektrode in der Weise fest verbunden ist, dass der digitale Datenspeicher mit der pH-Elektrode eine nicht trennbare Einheit bildet, wobei der Datenspeicher dazu ausgelegt ist, die Extremalwerte der Betriebstemperatur zu speichern; wobei der potentiometrische Sensor (1) über eine mechanische Kupplung, welche die Schnittstelle umfasst, lösbar mit einem Kabel zur Kommunikation mit der übergeordneten Einheit verbindbar ist; wobei die Schnittstelle eine Schnittstelle mit elektrisch leitenden Kontakten ist.
摘要:
In a method for determining the load impedance of a measuring transducer, which is supplied with voltage via a first pair of lines and transmits a variable current signal through a current loop to a control system via a second pair of lines, a test voltage signal is capacitively coupled into the current loop and the corresponding current signal is evaluated. The up-to-date value of the load impedance is determined from a characteristic of the current signal, particularly from the RC time constant. In this way, excess load impedance can already be recognized when starting up a measuring transducer.
摘要:
A method for operating a measuring point (1, 2, 3) having a base unit (11, 12, 13) and a sensor unit (21, 22, 23), said sensor unit being coupled to the base unit via a plug connector coupling; the base unit being used for power supply of the sensor unit, for data exchange with the sensor unit, and for communication with a process monitoring facility, said base unit comprising: a first element, which has a first microprocessor for sensor data preparation for communication to the process monitoring facility (30, 31); a first data memory for storing data specific to the measuring point; said sensor unit having a primary sensor and a sensor head; the primary sensor comprising a converter which outputs a signal as a function of a measured variable; said sensor head comprising: a circuit for preparing the converter signals using an A/D converter and a second microprocessor for measurement signal processing, a second data memory for sensor data; a program memory, which contains firmware; and an interface having a second element of the plug connector coupling; a software module, which allows supplementary functions to the primary sensor monitoring, being transmitted from said base unit to said sensor unit and being stored in said program memory of said sensor unit.
摘要:
The invention relates to a system for process automation with a plurality of intelligent sensors (2.1... 2.n), wherein each sensor (2.1... 2.n) is used to determine or monitor a physical or chemical process variable of a medium (6) and comprises, respectively a primary-side plug-type connector element (3.1... 3.n) and a secondary-side plug-type connector element (4.1... 4.n) provided with a sensor element. The power supply and the data communication between both of the plug-type connector elements (3.1... 3.n; 4.1... 4.n) occurs via a releasable plug-type connector coupling (7.1... 7.n). Each sensor (2.1... 2.n) is associated with a web service interface (8.1... 8.n) via which the sensor (2.1... 2.n) can be connected to a wide area network (WAN) or to a local area network (LAN). A control unit (10) or a server, which provides at least one piece of software for generating a virtual measuring transducer (VM), is provided. The virtual measuring transducer (VM) and the sensors (2.1... 2.n) communicate via the web service-interface (8.1... 8.n).
摘要:
Die Erfindung offenbart einen Sensor, an welchen mittels eines Steckverbinders 1 eine Übertragungsleitung anschließbar ist, wobei der Steckverbinder 1 ein Steckerelement 4 und ein mit dem Steckerelement 4 lösbar verbindbares Buchsenelement 5, zum Anschluss der Übertragungsleitung 2 an den Sensor 3 aufweist, das Steckerelement 4 oder das Buchsenenelement mittels einer elektrischen Kontaktierung 7 an den Sensor 3 angeschlossen ist, und eine geeignete Abdichtung im Bereich des Anschlusses vorgesehen ist, um das Eindringen von Feuchtigkeit, Staub oder Schmutzpartikeln und ätzender Substanzen in den Bereich der elektrischen Kontaktierung 7 zu verhindern, die Übertragungsleitung 2 an das jeweils andere Element des Steckverbinders 1 mittels einer elektrischen Kontaktierung 6 angeschlossen ist, und der Anschluss durch geeignete Mittel abgedichtet ist, um ein Eindringen von Feuchtigkeit, Staub oder Schmutzpartikeln und aggressiver oder ätzender Substanzen in den Bereich der elektrischen Kontaktierung 6 zu verhindern.