公开(公告)号：EP0931160B1

公开(公告)日：2008-12-17

申请号：EP96931558.9

申请日：1996-09-13

申请人： Garvin, Alex M.

发明人： Garvin, Alex M.

IPC分类号： C12P19/34 ,  G01N33/543 ,  C12Q1/68

CPC分类号： C12Q1/68 ,  C12Q1/6804 ,  C12Q2531/143 ,  C12Q2525/149

摘要： A process for detecting protein altering mutations in genes. Coding sequence of the gene is PCR amplified with a 5' primer that contains at its 5' end a polymerase binding site, a translation initiation site, and an in frame sequence coding for a peptide tag, followed by the in frame 5' end of the test sequence. After PCR amplification of the test sequence, the PCR product is used as template to make mRNA in an in vitro transcription reaction using an RNA polymerase that recognizes the polymerase binding site incorporated into the 5' PCR primer. The mRNA is then used as template to make protein in an in vitro translation reaction. The protein encoded by the test sequence has at its amino terminus a peptide tag that can be used to either detect the protein, or to purify the protein for further analysis.

公开(公告)号：EP1272628A2

公开(公告)日：2003-01-08

申请号：EP01938085.6

申请日：2001-04-04

申请人： Garvin, Alex M.

发明人： Garvin, Alex M.

IPC分类号： C12N15/10 ,  C12Q1/68 ,  C07H1/08

CPC分类号： C12N15/1017

摘要： For reducing side effects of blood transfusion, white (nucleated) blood cells are separated from donated human blood by filtering the blood through filter media selectively adsorbing white blood cells. Such filter media are processed for recovering nucleic acids contained in the white blood cells. The cells retained by the filter media are separated from the filter media e.g. by washing with an aqueous solution. The separated cells are then lysed and the nucleic acids are isolated from the components of the lysed cells. Preferably each portion of filter medium is used for filtering the blood of one individual donor and is processed separately whereby samples of nucleic acids of one individual each are obtained.

公开(公告)号：EP4349179A1

公开(公告)日：2024-04-10

申请号：EP22000226.5

申请日：2022-10-07

申请人： Garvin, Alex M.

发明人： Garvin, Alex M.

IPC分类号： A23C19/097 ,  A23C19/14 ,  A23C19/068

摘要： The present invention provides methods for the treatment of larvae infested cheese in order to kill said larvae and other pathogens thus rendering the cheese safe for human consumption.
This inventive method has utility in the food science field.

公开(公告)号：EP0931160A1

公开(公告)日：1999-07-28

申请号：EP96931558.0

申请日：1996-09-13

申请人： Garvin, Alex M.

发明人： Garvin, Alex M.

IPC分类号： C12Q1

CPC分类号： C12Q1/68 ,  C12Q1/6804 ,  C12Q2531/143 ,  C12Q2525/149

摘要： A process for detecting protein altering mutations in genes. Coding sequence of the gene is PCR amplified with a 5' primer that contains at its 5' end a polymerase binding site, a translation initiation site, and an in frame sequence coding for a peptide tag, followed by the in frame 5' end of the test sequence. After PCR amplification of the test sequence, the PCR product is used as template to make mRNA in an in vitro transcription reaction using an RNA polymerase that recognizes the polymerase binding site incorporated into the 5' PCR primer. The mRNA is then used as template to make protein in an in vitro translation reaction. The protein encoded by the test sequence has at its amino terminus a peptide tag that can be used to either detect the protein, or to purify the protein for further analysis.

公开(公告)号：EP0931160A4

公开(公告)日：2004-10-06

申请号：EP96931558

申请日：1996-09-13

申请人： GARVIN ALEX M

发明人： GARVIN ALEX M

IPC分类号： C12Q1/68 ,  C12P19/34 ,  A61K38/00

CPC分类号： C12Q1/68 ,  C12Q1/6804 ,  C12Q2531/143 ,  C12Q2525/149

摘要： A process for detecting protein altering mutations in genes. Coding sequence of the gene is PCR amplified with a 5' primer that contains at its 5' end a polymerase binding site, a translation initiation site, and an in frame sequence coding for a peptide tag, followed by the in frame 5' end of the test sequence. After PCR amplification of the test sequence, the PCR product is used as template to make mRNA in an in vitro transcription reaction using an RNA polymerase that recognizes the polymerase binding site incorporated into the 5' PCR primer. The mRNA is then used as template to make protein in an in vitro translation reaction. The protein encoded by the test sequence has at its amino terminus a peptide tag that can be used to either detect the protein, or to purify the protein for further analysis.