公开(公告)号：EP3431605B1

公开(公告)日：2024-11-06

申请号：EP17766877.9

申请日：2017-01-05

发明人： LEE, Na Hum ,  LEE, Jae Hun ,  LI, Hong Xian ,  MOON, Jun Ok ,  UM, Hye Won

IPC分类号： C12N15/77 ,  C12N9/02 ,  C12N9/10 ,  C12N9/88 ,  C12P13/00 ,  C12R1/15

公开(公告)号：EP4452927A1

公开(公告)日：2024-10-30

申请号：EP22839723.8

申请日：2022-12-16

申请人： Covestro Deutschland AG

发明人： KRAUSE, Jonas ,  FRANCISCO CASAL, Maria ,  KLOECKNER, Wolf ,  BEDNARZ, Andreas

IPC分类号： C07C227/42 ,  C07C229/56 ,  C07C229/60 ,  C12P13/00

公开(公告)号：EP4448736A1

公开(公告)日：2024-10-23

申请号：EP22856985.1

申请日：2022-12-14

申请人： Covestro Deutschland AG

发明人： KLAFFL, Simon ,  SPRENGER, Georg ,  GOTTLIEB, Katrin ,  YOUN, Jung-Won

IPC分类号： C12N9/10 ,  C12N15/52 ,  C12P13/22

公开(公告)号：EP4446420A1

公开(公告)日：2024-10-16

申请号：EP22904228.8

申请日：2022-12-06

申请人： Tokyo Women's Medical University

发明人： HASUNUMA, Tomohisa ,  KATO, Yuichi ,  INABE, Kosuke ,  KONDO, Akihiko ,  SHIMIZU, Tatsuya ,  HARAGUCHI, Yuji

IPC分类号： C12P13/04 ,  C12P19/04 ,  C12N5/07 ,  C12N5/077 ,  C12N5/10 ,  C12N1/00 ,  C12N1/12 ,  C12N1/13 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N15/31 ,  C12N15/52 ,  C12N15/53 ,  C12Q1/04 ,  C12Q1/68 ,  C12P7/56 ,  C12P7/62 ,  A23L13/00

摘要： According to the present disclosure, there is provided a system for culturing animal cells using a component derived from an organism such as algae as a nutrient source, and reusing the culture waste liquid. The present disclosure provides an organism or a cultured cell which undergoes a modification, in which the modification includes imparting or enhancing L-lactate utilization ability in the organism or the cultured cell. In addition, the present disclosure provides a method for culturing at least two types of cells including a cell X and a cell Y in a circulation manner, the method including: a step (a) of providing a component excreted from the cell X to the cell Y; a step (b) of providing a component derived from the cell Y to the cell X; a step (c) of culturing the cell X and the cell Y; and a step (d) of repeating the steps (a) to (c) as necessary, in which at least one of the components excreted from the cell X is an assimilable component of the cell Y, and at least one of the components derived from the cell Y is a nutritional component of the cell X.

公开(公告)号：EP4438592A1

公开(公告)日：2024-10-02

申请号：EP22964190.7

申请日：2022-11-21

申请人： Shanghai Bios Technology Co., Ltd.

发明人： YU, Wansheng ,  RUAN, Libo ,  PENG, Qiujun ,  ZENG, Yifei

IPC分类号： C07C271/22 ,  C07C269/00 ,  C07C247/16 ,  C12P13/02 ,  C07D307/00

CPC分类号： Y02P20/55

摘要： The present invention discloses an edoxaban intermediate, and a preparation method therefor. A general structural formula of the edoxaban intermediate is

or

wherein R1 is OH, alkoxy or a N,N-dialkyl group, and R2 is hydrogen, alkoxycarbonyl or an amino protecting group. The preparation method includes subj ecting

and an oxidizing agent to an oxidation reaction to obtain a compound of a formula II; and mixing the compound of the formula II, an aminotransferase, an aminotransferase coenzyme, and a phosphate buffer solution for an enzyme catalyzed reaction or further amine derivatization to obtain a compound of a formula I. Compared with chemical synthesis methods in the prior art, such key edoxaban intermediates and the preparation method therefor provided by the present invention have the advantages of a novel structure, mild reaction conditions, and high yield, having good industrial values.

公开(公告)号：EP4433603A1

公开(公告)日：2024-09-25

申请号：EP22821332.8

申请日：2022-11-17

申请人： METABOLIC EXPLORER

发明人： VASSEUR, Perrine ,  DESFOUGERES, Thomas ,  RAYNAUD, Céline

IPC分类号： C12P13/08 ,  C12N9/88 ,  C12R1/19 ,  C07K14/245 ,  C12N15/52

CPC分类号： C12P13/08 ,  C12N9/88 ,  C12Y403/01019 ,  C12R2001/1920210501 ,  C07K14/245 ,  C12N15/52

公开(公告)号：EP4433602A1

公开(公告)日：2024-09-25

申请号：EP22894614.1

申请日：2022-10-30

申请人： Enzymaster (Ningbo) Bio-Engineering Co., Ltd.

发明人： ZHANG, Yingxin ,  CHEN, Haibin ,  BOCOLA, Marco ,  CAI, Baoqin ,  ZHANG, Zhaoqi ,  LUO, Xiao ,  JI, Yaoyao ,  ZHANG, Chengxiao ,  HONG, Ruimei

IPC分类号： C12P13/02 ,  C12N9/86 ,  C12N15/55

CPC分类号： C12Y305/02002 ,  C12N9/86 ,  C12P13/02 ,  C12P13/04 ,  C12P41/009

公开(公告)号：EP4414448A1

公开(公告)日：2024-08-14

申请号：EP22912896.2

申请日：2022-01-24

申请人： Asymchem Laboratories (Tianjin) Co., Ltd.

发明人： HONG, Hao ,  JAMES, Gage ,  ZHANG, Na ,  JIAO, Xuecheng ,  LI, Rui ,  YANG, Yiming ,  ZHANG, Yanqing

IPC分类号： C12N9/10 ,  C12N15/54 ,  C12N1/21 ,  C12N1/19 ,  C12P17/04 ,  C12P13/00 ,  C12P17/00

CPC分类号： C12P17/04 ,  C12P13/00 ,  C12N9/10 ,  C12R2001/1920210501 ,  C12N15/81 ,  C12N15/74 ,  C12P17/00

摘要： Disclosed are a transaminase mutant and the use thereof, herein the transaminase mutant has a sequence formed after an amino acid mutation occurs in a sequence as shown in SEQ ID NO: 1, and the site at which the amino acid mutation occurs includes a V242W site. On the basis of transaminase shown in SEQ ID NO: 1, the transaminase mutant undergoes the mutation by means of a site-directed mutation method, thereby the amino acid sequence of the transaminase is changed and changes in protein structure and function are achieved, and then the transaminase with the above mutation site is obtained by means of a directed screening method. The transaminase obtained has relatively high catalytic activity, specific selectivity, wide substrate spectrum and broad industrial prospect.

公开(公告)号：EP4077695B1

公开(公告)日：2024-07-24

申请号：EP20823810.5

申请日：2020-12-14

IPC分类号： C12P13/04 ,  C12N9/00 ,  C12N9/10

CPC分类号： C12N9/1003 ,  C12Y201/04001 ,  C12Y201/03003 ,  C12Y403/02001 ,  C12Y603/04005 ,  C12Y201/01002 ,  C12P13/04 ,  C12Y603/04016 ,  C12Y603/05005

公开(公告)号：EP4393939A1

公开(公告)日：2024-07-03

申请号：EP22887593.6

申请日：2022-10-26

申请人： CJ CheilJedang Corporation

发明人： KIM, Hyo Kyung ,  LEE, Zeewon ,  CHOI, Sun Hyoung

IPC分类号： C07K14/34 ,  C12N15/77 ,  C12P13/10

CPC分类号： C12N15/77 ,  C12P13/10 ,  C07K14/34

摘要： The present disclosure provides a LysE variant.