公开(公告)号：EP0770624B1

公开(公告)日：2003-05-02

申请号：EP96307163.4

申请日：1996-09-30

申请人： Ajinomoto Co., Inc. ,  Kikuchi, Kokichi

发明人： Kikuchi, Kokichi ,  Sato, Noriyuki ,  Sahara, Hiromitsu ,  Yasojima, Takahiro ,  Wada, Yoshimasa ,  Suzuki, Manabu, c/o Central Research Labs ,  Hamuro, Junji, c/o Central Research Labs

IPC分类号： C07K14/705 ,  A61K39/00 ,  C12N15/12 ,  C07K7/06

CPC分类号： C07K7/06 ,  A61K38/00 ,  A61K39/00 ,  C07H21/04 ,  C07K14/4748 ,  C07K14/705

公开(公告)号：EP0974653A2

公开(公告)日：2000-01-26

申请号：EP99305469.1

申请日：1999-07-09

申请人： Ajinomoto Co., Inc. ,  Kikuchi, Kokichi

发明人： Kikuchi, Kokichi ,  Sato, Noriyuki ,  Torigoe, Toshihiko ,  Sahara, Hiroeki ,  Suzuki, Manabu, c/o Ajinomoto Co., Inc. ,  Hamuro, Junji, c/o Ajinomoto Co., Inc.

IPC分类号： C12N15/12 ,  C07K14/47 ,  A61K38/17 ,  C07K7/06

CPC分类号： C07K14/4748 ,  A61K38/00 ,  A61K39/00

摘要： A tumor antigen gene is identified by screening a cDNA library derived from a gastric cancer cell line that can induce gastric cancer antigen specific cytotoxic T cell (CTL) by means of hybridization and PCR utilizing an amino acid sequence of peptide fragment of a known gastric cancer antigen protein, introducing a selected cDNA clone into a cell of gastric cancer cell line that cannot induce gastric cancer antigen specific CTL so that the clone should be expressed in the cell, and selecting a transgenic cell that has acquired the ability to induce CTL. According to the present invention, there are provided a protein capable of inducing immune response against human gastric cancer, DNA encoding the protein, as well as vaccine for treatment and prevention of human gastric cancer, and agent for treatment and prevention of human gastric cancer.

摘要翻译： 通过筛选来自胃癌细胞系的cDNA文库来鉴定肿瘤抗原基因，该cDNA文库可以通过使用已知胃癌的肽片段的氨基酸序列的杂交和PCR来诱导胃癌抗原特异性细胞毒性T细胞（CTL） 将选择的cDNA克隆引入到不能诱导胃癌抗原特异性CTL的胃癌细胞系的细胞中，使得克隆应在细胞中表达，并选择已经获得诱导CTL的能力的转基因细胞。 根据本发明，提供能够诱导针对人胃癌的免疫应答的蛋白质，编码该蛋白质的DNA，以及用于治疗和预防人胃癌的疫苗，以及用于治疗和预防人胃癌的药剂。

公开(公告)号：EP0974653B1

公开(公告)日：2005-09-14

申请号：EP99305469.1

申请日：1999-07-09

申请人： Ajinomoto Co., Inc. ,  Kikuchi, Kokichi

发明人： Kikuchi, Kokichi ,  Sato, Noriyuki ,  Torigoe, Toshihiko ,  Sahara, Hiroeki ,  Suzuki, Manabu, c/o Ajinomoto Co., Inc. ,  Hamuro, Junji, c/o Ajinomoto Co., Inc.

IPC分类号： C12N15/12 ,  C07K14/47 ,  A61K38/17 ,  C07K7/06

CPC分类号： C07K14/4748 ,  A61K38/00 ,  A61K39/00

公开(公告)号：EP0974653A3

公开(公告)日：2001-09-19

申请号：EP99305469.1

申请日：1999-07-09

申请人： Ajinomoto Co., Inc. ,  Kikuchi, Kokichi

发明人： Kikuchi, Kokichi ,  Sato, Noriyuki ,  Torigoe, Toshihiko ,  Sahara, Hiroeki ,  Suzuki, Manabu, c/o Ajinomoto Co., Inc. ,  Hamuro, Junji, c/o Ajinomoto Co., Inc.

IPC分类号： C12N15/12 ,  C07K14/47 ,  A61K38/17 ,  C07K7/06

CPC分类号： C07K14/4748 ,  A61K38/00 ,  A61K39/00

摘要： A tumor antigen gene is identified by screening a cDNA library derived from a gastric cancer cell line that can induce gastric cancer antigen specific cytotoxic T cell (CTL) by means of hybridization and PCR utilizing an amino acid sequence of peptide fragment of a known gastric cancer antigen protein, introducing a selected cDNA clone into a cell of gastric cancer cell line that cannot induce gastric cancer antigen specific CTL so that the clone should be expressed in the cell, and selecting a transgenic cell that has acquired the ability to induce CTL. According to the present invention, there are provided a protein capable of inducing immune response against human gastric cancer, DNA encoding the protein, as well as vaccine for treatment and prevention of human gastric cancer, and agent for treatment and prevention of human gastric cancer.

公开(公告)号：EP0770624A3

公开(公告)日：1998-12-23

申请号：EP96307163

申请日：1996-09-30

申请人： AJINOMOTO KK ,  KIKUCHI KOKICHI

发明人： KIKUCHI KOKICHI ,  SATO NORIYUKI ,  SAHARA HIROMITSU ,  YASOJIMA TAKAHIRO ,  WADA YOSHIMASA ,  SUZUKI MANABU ,  HAMURO JUNJI

IPC分类号： C12N15/09 ,  A61K35/14 ,  A61K35/76 ,  A61K38/00 ,  A61K39/00 ,  A61K48/00 ,  A61P1/00 ,  A61P35/00 ,  A61P37/00 ,  A61P43/00 ,  C07H21/04 ,  C07K7/06 ,  C07K14/47 ,  C07K14/705 ,  C07K14/82 ,  C12N1/21 ,  C12N7/00 ,  C12N15/12 ,  C12R1/19

CPC分类号： C07K7/06 ,  A61K38/00 ,  A61K39/00 ,  C07H21/04 ,  C07K14/4748 ,  C07K14/705

摘要： A peptide which is a fragment of a gastric cancer antigen or modification thereof, eg having the amino-acid sequence SEQ ID NO:1 or SEQ ID NO:2, when bound to an HLA molecule induces a cytotoxic T-cell response that targets a gastric cancer cell.
Accordingly, this peptide is expected to be useful as an agent for preventing or treating a gastric cancer.

公开(公告)号：EP0770624A2

公开(公告)日：1997-05-02

申请号：EP96307163.4

申请日：1996-09-30

申请人： Ajinomoto Co., Inc. ,  Kikuchi, Kokichi

发明人： Kikuchi, Kokichi ,  Sato, Noriyuki ,  Sahara, Hiromitsu ,  Yasojima, Takahiro ,  Wada, Yoshimasa ,  Suzuki, Manabu, c/o Central Research Labs ,  Hamuro, Junji, c/o Central Research Labs

IPC分类号： C07K14/705 ,  A61K39/00 ,  C12N15/12

CPC分类号： C07K7/06 ,  A61K38/00 ,  A61K39/00 ,  C07H21/04 ,  C07K14/4748 ,  C07K14/705

摘要： A peptide which is a fragment of a gastric cancer antigen or modification thereof, eg having the amino-acid sequence SEQ ID NO:1 or SEQ ID NO:2, when bound to an HLA molecule induces a cytotoxic T-cell response that targets a gastric cancer cell.
Accordingly, this peptide is expected to be useful as an agent for preventing or treating a gastric cancer.

摘要翻译： 的肽的所有其是胃癌抗原或变体的片段，例如具有氨基酸序列SEQ ID NO：1或SEQ ID NO：2，当绑定到HLA分子诱导细胞毒性T细胞应答做目标的 胃癌细胞。 因此，该肽被认为其作为用于预防或治疗胃癌的药剂是有用的。