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公开(公告)号：EP3372674A1
公开(公告)日：2018-09-12
申请号：EP16862024.3
申请日：2016-10-31
摘要： The present invention provides a method for producing platelets, including the step of culturing megakaryocyte cells in a culture solution in a culture vessel, wherein in the culturing step, the culture solution is stirred by a stirring blade moving reciprocally.
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公开(公告)号：EP3312270A1
公开(公告)日：2018-04-25
申请号：EP16811730.7
申请日：2016-06-16
发明人： ETO, Koji , NAKAMURA, Sou , ITO, Yukitaka , SHIGEMORI, Tomohiro , DOHDA, Takeaki
IPC分类号： C12N5/078 , A61K35/19 , A61P7/04 , C12N15/09 , C12N15/113
摘要： The present invention provides a highly functional platelet production promoting agent which comprises one or a plurality of aryl hydrocarbon receptor (AhR) antagonists and one or a plurality of Rho-associated coiled-coil forming kinase (ROCK) inhibitors.
摘要翻译： 本发明提供高功能血小板产生促进剂，其包含一种或多种芳基烃受体（AhR）拮抗剂和一种或多种Rho相关卷曲螺旋形成激酶（ROCK）抑制剂。
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公开(公告)号：EP3950921A1
公开(公告)日：2022-02-09
申请号：EP20778817.5
申请日：2020-03-24
发明人： HIGUCHI, Akira , NAITO, Hiroyuki , OKAMOTO, Haruki
摘要： A cell suspension treatment apparatus has a circulation circuit and a filling liquid supply source that supplies a filling liquid to the circulation circuit. The circulation circuit includes a hollow fiber membrane filter, a reservoir, a pump that is provided on a downstream side of the reservoir and on an upstream side of the hollow fiber membrane filter, and a valve that is provided on the downstream side of the reservoir and on an upstream side of the pump. The filling liquid supply source is connected to a portion of the circulation circuit between the first valve and the pump. After the concentrated cell suspension is stored in the reservoir, in a state where the valve is closed and the pump is driven, the filling liquid supply source starts supplying the filling liquid to the circulation circuit to push the cell suspension, which remains in a portion of the circulation circuit from the pump to the inlet port of the reservoir, to flow toward the reservoir by the filling liquid.
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4.发明公开
公开(公告)号：EP3680325A1
公开(公告)日：2020-07-15
申请号：EP18859680.3
申请日：2018-09-19
摘要： The present invention provides a method for producing platelets that can improve at least one of the ability of megakaryocyte to produce platelets and the bioactivity of platelets produced even in high-density culture, for example. The method for producing platelets of the present invention includes a platelet producing step of producing platelets from megakaryocytes, wherein the platelet producing step is performed in the presence of at least one of glycine and cysteine.
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公开(公告)号：EP4063489A1
公开(公告)日：2022-09-28
申请号：EP20899161.2
申请日：2020-12-11
IPC分类号： C12N5/078 , A61K35/19 , A61K38/18 , A61P17/00 , A61P17/02 , A61P17/14 , A61P19/02 , A61P19/04 , A61P43/00 , C12N5/0775
摘要： A composition having cell-derived physiological activity is provided. The composition according to the present invention contains a treated product of megakaryocytes or a culture of the megakaryocytes.
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公开(公告)号：EP3940061A1
公开(公告)日：2022-01-19
申请号：EP20773511.9
申请日：2020-03-13
发明人： TOMIZUKA, Junko , SHIGEMORI, Tomohiro , WATANABE, Natsuki , TAKENAWA, Taichi , SHIRAKAWA, Chikage , NISHIMURA, Masuhiro , FUJITA, Yasutaka , SAWAMOTO, Osamu
IPC分类号： C12N5/0775 , C12N5/078 , C12N5/0783 , C12N1/04
摘要： An object of the present invention is to provide a novel preservation solution for preserving mammalian cells over a long period of time in a non-frozen state. Ascorbic acid and/or niacin is added to an isotonic solution to prepare a preservation solution. The preservation solution is mixed with platelets and preserved under shaking. Decrease in the functions and viability of the platelets can thereby be suppressed for at least 10 days. Alternatively, the aforementioned preservation solution is mixed with mesenchymal stem cells, megakaryocytes, or T cells and preserved in a non-frozen state. Decrease in the functions and viability of these cells can thereby be suppressed for at least several days to several tens of days.
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公开(公告)号：EP3372674B1
公开(公告)日：2020-10-07
申请号：EP16862024.3
申请日：2016-10-31
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公开(公告)号：EP3312270B1
公开(公告)日：2020-09-30
申请号：EP16811730.7
申请日：2016-06-16
发明人： ETO, Koji , NAKAMURA, Sou , ITO, Yukitaka , SHIGEMORI, Tomohiro , DOHDA, Takeaki
IPC分类号： C12N5/078 , A61K35/19 , A61P7/04 , C12N15/09 , C12N15/113
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公开(公告)号：EP4342982A1
公开(公告)日：2024-03-27
申请号：EP22825104.7
申请日：2022-06-17
发明人： ITO, Yukitaka , MISUMI, Sachiyo
摘要： A method for improving the amount of platelets produced. The production method is a method for producing multinucleated megakaryocyte cells with enhanced platelet production capability, the method including a multinucleation step of inducing multinucleated megakaryocyte cells through multinucleation of pre-multinucleated megakaryocyte cells in the presence of a growth factor of the pre-multinucleated megakaryocyte cells. In the multinucleation step, the multinucleated megakaryocyte cells are induced by suppressing the activity of at least one protein selected from the group consisting of an aryl hydrocarbon receptor (AhR), a Rho-associated kinase (ROCK), dual-specificity tyrosine phosphorylation-regulated kinases (DYRK), and myosin 2, and multinucleating the pre-multinucleated megakaryocyte cells in the presence of harmine.
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10.发明公开
公开(公告)号：EP3650535A1
公开(公告)日：2020-05-13
申请号：EP18828313.9
申请日：2018-07-05
摘要： Methods of production and apparatuses for production for stably producing healthy and functional platelets, and methods for determining operating conditions of an apparatus for producing platelets are provided. The present invention relates to methods of production comprising a step of culturing megakaryocytes in a platelet production medium C, wherein the step of culturing comprises a step of stirring a medium C in containers 1, 4 using stirring mechanisms 2, 5. In one method of production, the step of stirring comprises reciprocating stirring blades 21, 24 in the stirring mechanisms 2, 24 such that any of predetermined turbulent energy, shearing stresses, or Kolmogorov scale is produced, in the medium C. In another method of production, the step of stirring comprises pivoting a stirring blade 51 in the stirring mechanism 5 wherein a plurality of stationary members 53 is placed therearound in a bottom region 4e of the container 4 such that the step of stirring produces turbulence in the medium C. The present invention also relates to apparatuses for production for carrying out the method of production. The present invention also relates to a method for determining operating conditions of an apparatus for producing platelets.
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