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公开(公告)号：EP3312270A1
公开(公告)日：2018-04-25
申请号：EP16811730.7
申请日：2016-06-16
发明人： ETO, Koji , NAKAMURA, Sou , ITO, Yukitaka , SHIGEMORI, Tomohiro , DOHDA, Takeaki
IPC分类号： C12N5/078 , A61K35/19 , A61P7/04 , C12N15/09 , C12N15/113
摘要： The present invention provides a highly functional platelet production promoting agent which comprises one or a plurality of aryl hydrocarbon receptor (AhR) antagonists and one or a plurality of Rho-associated coiled-coil forming kinase (ROCK) inhibitors.
摘要翻译： 本发明提供高功能血小板产生促进剂，其包含一种或多种芳基烃受体（AhR）拮抗剂和一种或多种Rho相关卷曲螺旋形成激酶（ROCK）抑制剂。
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2.发明公开
公开(公告)号：EP3650535A1
公开(公告)日：2020-05-13
申请号：EP18828313.9
申请日：2018-07-05
摘要： Methods of production and apparatuses for production for stably producing healthy and functional platelets, and methods for determining operating conditions of an apparatus for producing platelets are provided. The present invention relates to methods of production comprising a step of culturing megakaryocytes in a platelet production medium C, wherein the step of culturing comprises a step of stirring a medium C in containers 1, 4 using stirring mechanisms 2, 5. In one method of production, the step of stirring comprises reciprocating stirring blades 21, 24 in the stirring mechanisms 2, 24 such that any of predetermined turbulent energy, shearing stresses, or Kolmogorov scale is produced, in the medium C. In another method of production, the step of stirring comprises pivoting a stirring blade 51 in the stirring mechanism 5 wherein a plurality of stationary members 53 is placed therearound in a bottom region 4e of the container 4 such that the step of stirring produces turbulence in the medium C. The present invention also relates to apparatuses for production for carrying out the method of production. The present invention also relates to a method for determining operating conditions of an apparatus for producing platelets.
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3.发明公开METHOD FOR PRODUCING STEM CELL CLONES SUITABLE FOR INDUCTION OF DIFFERENTIATION INTO SOMATIC CELLS 审中-公开生产干细胞克隆的方法，适用于诱导向体细胞的分化
公开(公告)号：EP3296390A1
公开(公告)日：2018-03-21
申请号：EP16780120.8
申请日：2016-04-14
发明人： ETO, Koji , ENDO, Hiroshi , SHIGEMORI, Tomohiro
CPC分类号： C12N5/0696 , C12N5/0644 , C12N2506/11 , C12N2506/45 , C12N2510/00
摘要： Provided is a method for producing a stem cell clone, which comprises the steps of:
(i) introducing into stem cells an exogenous gene associated with induction of differentiation into somatic cells;
(ii) inducing differentiation of the stem cells, introduced with an exogenous gene, into the somatic cells;
(iii) dedifferentiating the differentiation-induced somatic cells; and
(iv) isolating stem cells having the exogenous gene incorporated into a chromosome thereof from a colony of the stem cells formed in step (iii).摘要翻译： 提供了用于产生干细胞克隆的方法，其包括以下步骤：（i）将与诱导分化为体细胞相关的外源基因导入干细胞; （ii）诱导用外源基因导入的干细胞分化成体细胞; （iii）去分化分化诱导的体细胞; 和（iv）从步骤（iii）中形成的干细胞的集落中分离具有并入染色体中的外源基因的干细胞。
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公开(公告)号：EP3312270B1
公开(公告)日：2020-09-30
申请号：EP16811730.7
申请日：2016-06-16
发明人： ETO, Koji , NAKAMURA, Sou , ITO, Yukitaka , SHIGEMORI, Tomohiro , DOHDA, Takeaki
IPC分类号： C12N5/078 , A61K35/19 , A61P7/04 , C12N15/09 , C12N15/113
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5.发明公开
公开(公告)号：EP3680325A1
公开(公告)日：2020-07-15
申请号：EP18859680.3
申请日：2018-09-19
摘要： The present invention provides a method for producing platelets that can improve at least one of the ability of megakaryocyte to produce platelets and the bioactivity of platelets produced even in high-density culture, for example. The method for producing platelets of the present invention includes a platelet producing step of producing platelets from megakaryocytes, wherein the platelet producing step is performed in the presence of at least one of glycine and cysteine.
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公开(公告)号：EP3372674A1
公开(公告)日：2018-09-12
申请号：EP16862024.3
申请日：2016-10-31
摘要： The present invention provides a method for producing platelets, including the step of culturing megakaryocyte cells in a culture solution in a culture vessel, wherein in the culturing step, the culture solution is stirred by a stirring blade moving reciprocally.
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公开(公告)号：EP3351627A1
公开(公告)日：2018-07-25
申请号：EP16846363.6
申请日：2016-09-08
CPC分类号： C12N5/0644 , A61K35/14 , A61K35/19 , C12M3/00 , C12N2506/13 , C12N2527/00
摘要： The present invention provides a method for producing platelets, and the method comprises a step of culturing megakaryocytes in a culture solution in a culture vessel, wherein the culture solution is stirred with a stirrer in the culture step.
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公开(公告)号：EP3940061A1
公开(公告)日：2022-01-19
申请号：EP20773511.9
申请日：2020-03-13
发明人： TOMIZUKA, Junko , SHIGEMORI, Tomohiro , WATANABE, Natsuki , TAKENAWA, Taichi , SHIRAKAWA, Chikage , NISHIMURA, Masuhiro , FUJITA, Yasutaka , SAWAMOTO, Osamu
IPC分类号： C12N5/0775 , C12N5/078 , C12N5/0783 , C12N1/04
摘要： An object of the present invention is to provide a novel preservation solution for preserving mammalian cells over a long period of time in a non-frozen state. Ascorbic acid and/or niacin is added to an isotonic solution to prepare a preservation solution. The preservation solution is mixed with platelets and preserved under shaking. Decrease in the functions and viability of the platelets can thereby be suppressed for at least 10 days. Alternatively, the aforementioned preservation solution is mixed with mesenchymal stem cells, megakaryocytes, or T cells and preserved in a non-frozen state. Decrease in the functions and viability of these cells can thereby be suppressed for at least several days to several tens of days.
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公开(公告)号：EP3372674B1
公开(公告)日：2020-10-07
申请号：EP16862024.3
申请日：2016-10-31
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