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    PLATELET PRODUCTION METHOD AND APPARATUS AND METHOD FOR DETERMINING OPERATING CONDITIONS IN PLATELET PRODUCTION APPARATUS
    1.
    发明公开
    PLATELET PRODUCTION METHOD AND APPARATUS AND METHOD FOR DETERMINING OPERATING CONDITIONS IN PLATELET PRODUCTION APPARATUS 审中-公开

    公开(公告)号：EP3650535A1

    公开(公告)日：2020-05-13

    申请号：EP18828313.9

    申请日：2018-07-05

    申请人： Kyoto University ,  Megakaryon Corporation ,  Satake Chemical Equipment Mfg.,Ltd.

    发明人： ETO, Koji ,  NAKAMURA, Sou ,  ITO, Yukitaka ,  SHIGEMORI, Tomohiro ,  KATO, Yoshikazu

    IPC分类号： C12N5/078 ,  B01F7/16 ,  B01F7/18 ,  B01F11/00 ,  C12M1/02 ,  C12M3/00 ,  C12N5/10

    摘要： Methods of production and apparatuses for production for stably producing healthy and functional platelets, and methods for determining operating conditions of an apparatus for producing platelets are provided. The present invention relates to methods of production comprising a step of culturing megakaryocytes in a platelet production medium C, wherein the step of culturing comprises a step of stirring a medium C in containers 1, 4 using stirring mechanisms 2, 5. In one method of production, the step of stirring comprises reciprocating stirring blades 21, 24 in the stirring mechanisms 2, 24 such that any of predetermined turbulent energy, shearing stresses, or Kolmogorov scale is produced, in the medium C. In another method of production, the step of stirring comprises pivoting a stirring blade 51 in the stirring mechanism 5 wherein a plurality of stationary members 53 is placed therearound in a bottom region 4e of the container 4 such that the step of stirring produces turbulence in the medium C. The present invention also relates to apparatuses for production for carrying out the method of production. The present invention also relates to a method for determining operating conditions of an apparatus for producing platelets.

    METHOD FOR PRODUCING STEM CELL CLONES SUITABLE FOR INDUCTION OF DIFFERENTIATION INTO SOMATIC CELLS
    3.
    发明公开
    METHOD FOR PRODUCING STEM CELL CLONES SUITABLE FOR INDUCTION OF DIFFERENTIATION INTO SOMATIC CELLS 审中-公开
    生产干细胞克隆的方法，适用于诱导向体细胞的分化

    公开(公告)号：EP3296390A1

    公开(公告)日：2018-03-21

    申请号：EP16780120.8

    申请日：2016-04-14

    申请人： Kyoto University ,  Megakaryon Corporation

    发明人： ETO, Koji ,  ENDO, Hiroshi ,  SHIGEMORI, Tomohiro

    IPC分类号： C12N5/00 ,  C12N15/00 ,  C12N15/09

    CPC分类号： C12N5/0696 ,  C12N5/0644 ,  C12N2506/11 ,  C12N2506/45 ,  C12N2510/00

    摘要： Provided is a method for producing a stem cell clone, which comprises the steps of:
    (i) introducing into stem cells an exogenous gene associated with induction of differentiation into somatic cells;
    (ii) inducing differentiation of the stem cells, introduced with an exogenous gene, into the somatic cells;
    (iii) dedifferentiating the differentiation-induced somatic cells; and
    (iv) isolating stem cells having the exogenous gene incorporated into a chromosome thereof from a colony of the stem cells formed in step (iii).

    摘要翻译： 提供了用于产生干细胞克隆的方法，其包括以下步骤：（i）将与诱导分化为体细胞相关的外源基因导入干细胞; （ii）诱导用外源基因导入的干细胞分化成体细胞; （iii）去分化分化诱导的体细胞; 和（iv）从步骤（iii）中形成的干细胞的集落中分离具有并入染色体中的外源基因的干细胞。

    METHOD FOR PRODUCING PLATELETS
    5.
    发明公开
    METHOD FOR PRODUCING PLATELETS 审中-公开

    公开(公告)号：EP3594325A1

    公开(公告)日：2020-01-15

    申请号：EP18763555.2

    申请日：2018-03-05

    申请人： Kyoto University ,  Megakaryon Corporation

    发明人： ETO, Koji ,  NAKAMURA, Sou ,  ITO, Yukitaka

    IPC分类号： C12N5/078 ,  C07K14/52 ,  C12N5/10

    摘要： Healthy functional platelets are mass produced. A method for producing platelets, comprising: (1) a culture step of culturing megakaryocytes in a platelet producing medium in the presence of mechanical stress and platelet production promoting factors including MIF, NRDc, IGFBP2, TSP-1, PAI-1, and CCL5, and (2) a harvest step of harvesting the platelets obtained by the culture step; wherein the culture step comprises: (a) a step of promoting a release of the platelet production promoting factors from megakaryocytes by mechanical stress; and/or (b) a step of externally adding platelet production promoting factors including MIF, NRDc, and IGFBP2.