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    METHOD FOR IMPROVING THE FREEZING TOLERANCE OF PLANTS
    1.
    发明公开
    METHOD FOR IMPROVING THE FREEZING TOLERANCE OF PLANTS 失效
    方法改良植物的耐霜冻的

    公开(公告)号：EP0998199A1

    公开(公告)日：2000-05-10

    申请号：EP98931854.8

    申请日：1998-07-02

    申请人： UNIVERSITE DU QUEBEC A MONTREAL

    发明人： ALLARD, France ,  SARHAN, Fathey ,  HOUDE, Mario

    IPC分类号： A01N37/44

    CPC分类号： A01N37/44

    摘要： The accumulation of the osmolyte glycine-betaine is associated with increased freezing tolerance (FT) in wheat. An exogenous application of betaine at room temperature increased their FT by more than 5 °C. The treated plants showed some of the characteristics of cold-acclimated plants and expressed the low temperature responsive gene Wcor410 encoding a major membrane associated protein. The combined exposure to low temperature and betaine resulted in a cumulative effect on the improvement of FT surpassing the genetic potential of the plant to withstand freezing. This important finding indicates that an exogenous application of betaine before a predicted frost may be exploited to substantially improve cold or freezing tolerance in sensitive plants. Also disclosed is a method of reducing the growth rate of a plant by at least 30 %, which comprises the step of treating the plant with an effective dosage regimen of betaine or derivative thereof which is not lethal, preferably non-toxic to the plant. When growing the spring wheat variety Glenlea, in the presence of 500 mM of glycine betaine for four days, the growth rate thereof was reduced by about 75 %. Another aspect of the present invention is a method of inhibiting the growth of a plant, which comprises the step of treating said plant with a high dose regimen or betaine or derivative thereof. At lethal doses, this method results in killing undesirable plants. Another aspect of the present invention is a method of improving the germination rate of plant seeds at a temperature which is higher than about 0 °C but not lower than the coldest temperature that the plant seeds can withstand, which comprises the steps of administering to the seeds an effective dosage regimen of betaine or derivative thereof, and allowing the same to germinate at cold temperatures.

    ALBUMIN BASED HYDROGEL
    2.
    发明公开
    ALBUMIN BASED HYDROGEL 失效
    水凝胶BASED白蛋白

    公开(公告)号：EP0705298A1

    公开(公告)日：1996-04-10

    申请号：EP95901297.0

    申请日：1994-11-24

    申请人： UNIVERSITE DU QUEBEC A MONTREAL

    发明人： FORTIER, Guy

    IPC分类号： A61L15 ,  C08H1 ,  C08J3 ,  C08L89 ,  G02B1

    CPC分类号： G02B1/043 ,  A61L15/26 ,  A61L15/32 ,  A61L15/60 ,  C08H1/00 ,  C08J3/075 ,  C08L89/00 ,  C08L2666/02 ,  C08L71/02

    摘要： Novel bioartificial hydrogels consisting of a three-dimensional crosslinked mixture of: (a) a bifunctionalized polyethylene oxide, activated with an activating agent, dissolved in an aqueous solution; and (b) albumin type protein. The novel hydrogels are based on the crosslinking of albumin type protein of various sources including, for example, bovine serum albumin, lactalbumin or ovalbumin, with a bifunctionalyzed polyethylene oxide, most proferably polyethylene glycol, or a mixture of bifunctionalyzed polyethylene oxides preferably polyethylene glycol, of various molecular masses (Mr 2,000 to 35,000), dissolved in aqueous solution in adequate proportions. Also provided is a method and conditions for preparing the novel hydrogels. Also divulged are a variety of biomedical applications for the novel hydrogels. Additionally, it has been found that the mechanical properties of the novel hydrogels can be improved by adding to the casting solution unreactive polyethylen glycol or other inert polymer of high molecular masses (Mr > 100,000). In general terms the novel hydrogels possess advantageous properties such as shape retention and shape memory, high water content (more than 94 % (w/w) based on the dry weight of the hydrogel), good mechanical and optical properties. The hydrogels exhibit additional charcteristics which may render them extremely useful in the pharmaceutic and medical areas due to other advantageous properties such as biocompatibility, resistance to proteases action, slow release of various drugs, hydrophillic surface, good oxygen permeability, controlled porosity.

    ENHANCEMENT OF FREEZING TOLERANCE IN TRANSGENIC PLANTS
    3.
    发明公开
    ENHANCEMENT OF FREEZING TOLERANCE IN TRANSGENIC PLANTS 审中-公开
    改善转基因植物中耐霜冻

    公开(公告)号：EP1339857A2

    公开(公告)日：2003-09-03

    申请号：EP01982000.0

    申请日：2001-10-29

    申请人： UNIVERSITE DU QUEBEC A MONTREAL

    发明人： SARHAN, Fathey ,  HOUDE, Mario ,  DALLAIRE, Sylvain ,  N'DONG, Daniel

    IPC分类号： C12N15/82

    CPC分类号： C07K14/415 ,  C12N15/8273

    摘要： The present invention relates to the transformation of a plant cell with a nucleic acid encoding a cold tolerance polypeptide from the Wcor410 family, possibly combined with a period of low temperature exposure, for increasing its viability, for improving its low temperature, freezing or cross-stress tolerance as well as its membrane stability, fruit and leafy vegetable stability, seed stability and shelf life at low temperature and for improving plant productivity, resistance to osmotic or pathogen stress. More generally, it relates to the transformation of any cell with a nucleic acid encoding a cold tolerance polypeptide from the Wcor410 family for improving its metabolic stability and activity at low temperature or for its cryoprotection, as well as the protection of food or drug by the addition of a cold tolerance polypeptide from the Wcor410 family. A method for plant transformation based on high kanamycin selection pressure is also claimed.