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19 条记录 (耗时 0.012 秒)

    THERMOLABILE EXONUCLEASES
    2.
    发明公开
    THERMOLABILE EXONUCLEASES 审中-公开
    热可塑性外切变

    公开(公告)号：EP3183340A1

    公开(公告)日：2017-06-28

    申请号：EP15770444.6

    申请日：2015-08-19

    申请人： Arcticzymes AS ,  Universitetet I Tromsø - Norges Arktiske Universitet

    发明人： SOLSTAD, Terese ,  ANDREASSEN, Elisabeth, Lill ,  LORENTZEN, Marit, Sjo ,  LANES, Olav ,  ELDE, Morten ,  LARSEN, Atle, Noralf ,  PIOTROWSKI, Yvonne ,  WILLASSEN, Nils, Peder

    IPC分类号： C12N9/22

    CPC分类号： C12Q1/6848 ,  C12N9/22 ,  C12Q1/34 ,  C12Q2521/325 ,  C12Y301/11001 ,  G01N1/30 ,  G01N2333/922

    摘要： The invention provides an exonuclease or an enzymatically active fragment thereof, said exonuclease having the amino acid sequence of SEQ ID No. 1 or an amino acid sequence which is at least about 50% identical thereto, wherein said exonuclease or enzymatically active fragment thereof (i) is substantially irreversibly inactivated by heating at a temperature of about 55° C. for 10 minutes in a buffer consisting of 10 mM Tris-HCI, pH 8.5 at 25° C., 50 mM KCI and 5 mM MgCI2; (ii) is substantially specific for single stranded DNA; and (iii) has a 3′-5′ exonuclease activity. The invention further provides a method of removing single stranded DNA from a sample, a method of nucleic acid amplification, a method of reverse transcription and a method of nucleic acid sequence analysis in which the exonuclease or enzymatically active fragment thereof is used. The invention still further provides nucleic acids encoding said exonuclease or an enzymatically active fragment thereof and kits or compositions comprising the same.

    摘要翻译： 本发明提供了核酸外切酶或其酶促活性片段，所述外切核酸酶具有SEQ ID No.1的氨基酸序列或与其至少约50％同一性的氨基酸序列，其中所述外切核酸酶或其酶促活性片段（i ）通过在由pH为8.5的10mM Tris-HCl，25℃，50mM KCl和5mM MgCl 2组成的缓冲液中，在约55℃的温度下加热10分钟而基本上不可逆地失活; （ii）对单链DNA基本上是特异性的; 和（iii）具有3'-5'外切核酸酶活性。 本发明进一步提供了从样品中去除单链DNA的方法，核酸扩增方法，逆转录方法和核酸序列分析方法，其中使用了核酸外切酶或其酶活性片段。 本发明还进一步提供了编码所述外切核酸酶或其酶促活性片段的核酸和包含其的核酸酶或组合物。

    ANTI-LYMPHOMA PEPTIDES
    8.
    发明公开
    ANTI-LYMPHOMA PEPTIDES 审中-公开
    ANTI-淋巴癌肽

    公开(公告)号：EP3102229A1

    公开(公告)日：2016-12-14

    申请号：EP15703272.3

    申请日：2015-02-04

    申请人： Universitetet i Tromsø - Norges Arktiske Universitet

    发明人： SVENDSEN, John Sigurd ,  REKDAL, Øystein ,  EKSTEEN, Johannes

    IPC分类号： A61K39/00 ,  C07K7/06 ,  C07K7/08

    CPC分类号： C07K7/08 ,  A61K38/00 ,  A61K38/03 ,  C07K7/06

    摘要： The present invention provides a peptide which: (i) consists of 10 to 16 amino acids; (ii) has at least 8 cationic amino acids with either (a) a side chain comprising a guanidinium group, or (b) a side chain comprising an amino group, of which no more than 7 are consecutive; and (iii) has 2 or 3 tryptophan residues which are not both, or not all, consecutive, said peptide optionally in the form of a salt, ester or amide, or a peptidomimetic of said peptide. The invention further relates to pharmaceutical compositions containing these compounds, the use of these compounds in therapy, particularly as anti-tumour (e.g. anti-lymphoma) agents and non-therapeutic uses of these compounds.

    摘要翻译： 本发明提供一种肽，其：（i）由10至16个氨基酸组成; （ii）具有至少8个阳离子氨基酸，其具有（a）包含胍基的侧链，或（b）包含不超过7个连续的氨基的侧链; 和（iii）具有2或3个色氨酸残基，其不同时是或全部连续的，所述肽任选呈盐，酯或酰胺形式或所述肽的肽模拟物。 本发明还涉及包含这些化合物的药物组合物，这些化合物在治疗中的用途，特别是作为抗肿瘤（例如抗淋巴瘤）药物以及这些化合物的非治疗用途。