公开(公告)号：EP0326100A2

公开(公告)日：1989-08-02

申请号：EP89101263.5

申请日：1989-01-25

Applicant: ABBOTT LABORATORIES

Inventor： Hiltibran, Robert G. ,  Jou, Yi-Her ,  Kline, Steven J. ,  Schultz, Steven George ,  Stroupe, Stephen Denham

IPC: G01N33/537 ,  G01N33/543 ,  G01N33/538

CPC classification number: G01N33/538 ,  G01N33/54366 ,  G01N33/57473

Abstract: This invention presents novel separation and assay procedures which allows both the indicator and the capture reagents to be in solution to avoid problems of slowed immunoreaction kinetics. The separation procedure involves an analyte-specific soluble capture reagent, that is conjugated to a charged substance, and an insoluble solid phase material that is oppositely charged. A fluid sample suspected of containing the analyte is mixed with the capture reagent in solution to form a charged capture reagent/analyte complex. When binding is complete, the solution is contacted to the oppositely charged solid phase material to attract, attach, and separate the capture reagent/analyte complex from the fluid sample. With the appropriate indicator reagent, i.e., a second analyte-­specific binding substance which is conjugated to a label capable of producing a detectable signal, both sandwich and competitive assays can be performed. The assay reaction complex can be separated from the solution by contact with the oppositely charged solid phase material, and the presence or amount of analyte is monitored by detecting the label of the indicator reagent.

Abstract translation: 本发明提出了新的分离和测定方法，其允许指示剂和捕获试剂在溶液中以避免减慢的免疫反应动力学的问题。 分离过程包括与带电物质结合的分析物特异性可溶性捕获试剂和相反带电的不溶性固相材料。 将疑似含有分析物的流体样品与捕获试剂在溶液中混合以形成带电的捕获试剂/分析物复合物。 当结合完成时，溶液与相反电荷的固相材料接触以吸引，附着和从流体样品中分离捕获试剂/分析物复合物。 使用合适的指示剂，即与能够产生可检测信号的标签缀合的第二分析物特异性结合物质，可以进行夹心和竞争性测定。 通过与相反电荷的固相材料接触可以将测定反应复合物与溶液分离，并通过检测指示剂试剂的标记来监测分析物的存在或量。

公开(公告)号：EP0326100B1

公开(公告)日：1996-09-11

申请号：EP89101263.5

申请日：1989-01-25

Applicant: ABBOTT LABORATORIES

Inventor： Hiltibran, Robert G. ,  Jou, Yi-Her ,  Kline, Steven J. ,  Schultz, Steven George ,  Stroupe, Stephen Denham

IPC: G01N33/537 ,  G01N33/543 ,  G01N33/538

CPC classification number: G01N33/538 ,  G01N33/54366 ,  G01N33/57473

公开(公告)号：EP0664451A1

公开(公告)日：1995-07-26

申请号：EP95102406.6

申请日：1989-07-31

Applicant: ABBOTT LABORATORIES

Inventor： Hiltibran, Robert G. ,  Stroupe, Stephen Denham ,  Yi-Her, Jou

IPC: G01N33/538 ,  G01N33/543

CPC classification number: C08G69/10 ,  G01N33/538 ,  G01N33/543 ,  G01N33/54366 ,  G01N33/57473

Abstract: This invention presents novel polymeric anionic molecules and novel negatively charged capture reagents comprising the reaction products of said anionic molecules and a specific binding member for use in separation techniques and assay procedures wherein said activated polymeric anionic molecule comprises a compound having the formula:

wherein n is about 10 to about 500;
z is about 1 to about 6;
W is selected from the group consisting of H⁺, Na⁺, K⁺, Li⁺, amine salts, and derivatives thereof; and
X is a reactive group or a structure having a reactive group that enables the chemical binding of said activated polymer to a specific binding member.

Abstract translation: 本发明提出新颖的聚合阴离子分子和新颖的带负电荷的捕获试剂，其包含所述阴离子分子的反应产物和用于分离技术和测定程序的特异性结合成员，其中所述活化的聚合阴离子分子包含具有下式的化合物： 其中n为约10至约500; z为约1至约6; W选自H +，Na +，K +，Li +，胺盐及其衍生物; 并且X是具有能够使所述活化聚合物与特定结合构件化学结合的反应性基团的反应性基团或结构。

公开(公告)号：EP0326100A3

公开(公告)日：1990-11-07

申请号：EP89101263.5

申请日：1989-01-25

Applicant: ABBOTT LABORATORIES

Inventor： Hiltibran, Robert G. ,  Jou, Yi-Her ,  Kline, Steven J. ,  Schultz, Steven George ,  Stroupe, Stephen Denham

IPC: G01N33/537 ,  G01N33/543 ,  G01N33/538

CPC classification number: G01N33/538 ,  G01N33/54366 ,  G01N33/57473

Abstract: This invention presents novel separation and assay procedures which allows both the indicator and the capture reagents to be in solution to avoid problems of slowed immunoreaction kinetics. The separation procedure involves an analyte-specific soluble capture reagent, that is conjugated to a charged substance, and an insoluble solid phase material that is oppositely charged. A fluid sample suspected of containing the analyte is mixed with the capture reagent in solution to form a charged capture reagent/analyte complex. When binding is complete, the solution is contacted to the oppositely charged solid phase material to attract, attach, and separate the capture reagent/analyte complex from the fluid sample. With the appropriate indicator reagent, i.e., a second analyte-­specific binding substance which is conjugated to a label capable of producing a detectable signal, both sandwich and competitive assays can be performed. The assay reaction complex can be separated from the solution by contact with the oppositely charged solid phase material, and the presence or amount of analyte is monitored by detecting the label of the indicator reagent.