公开(公告)号：EP0326100A2

公开(公告)日：1989-08-02

申请号：EP89101263.5

申请日：1989-01-25

Applicant: ABBOTT LABORATORIES

Inventor： Hiltibran, Robert G. ,  Jou, Yi-Her ,  Kline, Steven J. ,  Schultz, Steven George ,  Stroupe, Stephen Denham

IPC: G01N33/537 ,  G01N33/543 ,  G01N33/538

CPC classification number: G01N33/538 ,  G01N33/54366 ,  G01N33/57473

Abstract: This invention presents novel separation and assay procedures which allows both the indicator and the capture reagents to be in solution to avoid problems of slowed immunoreaction kinetics. The separation procedure involves an analyte-specific soluble capture reagent, that is conjugated to a charged substance, and an insoluble solid phase material that is oppositely charged. A fluid sample suspected of containing the analyte is mixed with the capture reagent in solution to form a charged capture reagent/analyte complex. When binding is complete, the solution is contacted to the oppositely charged solid phase material to attract, attach, and separate the capture reagent/analyte complex from the fluid sample. With the appropriate indicator reagent, i.e., a second analyte-­specific binding substance which is conjugated to a label capable of producing a detectable signal, both sandwich and competitive assays can be performed. The assay reaction complex can be separated from the solution by contact with the oppositely charged solid phase material, and the presence or amount of analyte is monitored by detecting the label of the indicator reagent.

Abstract translation: 本发明提出了新的分离和测定方法，其允许指示剂和捕获试剂在溶液中以避免减慢的免疫反应动力学的问题。 分离过程包括与带电物质结合的分析物特异性可溶性捕获试剂和相反带电的不溶性固相材料。 将疑似含有分析物的流体样品与捕获试剂在溶液中混合以形成带电的捕获试剂/分析物复合物。 当结合完成时，溶液与相反电荷的固相材料接触以吸引，附着和从流体样品中分离捕获试剂/分析物复合物。 使用合适的指示剂，即与能够产生可检测信号的标签缀合的第二分析物特异性结合物质，可以进行夹心和竞争性测定。 通过与相反电荷的固相材料接触可以将测定反应复合物与溶液分离，并通过检测指示剂试剂的标记来监测分析物的存在或量。

公开(公告)号：EP0406473B1

公开(公告)日：1995-09-20

申请号：EP89114103.8

申请日：1989-07-31

Applicant: ABBOTT LABORATORIES

Inventor： Hiltibran Robert G. ,  Stroupe, Stephen Denham ,  Jou, Yi-Her

IPC: G01N33/538 ,  G01N33/543

CPC classification number: C08G69/10 ,  G01N33/538 ,  G01N33/543 ,  G01N33/54366 ,  G01N33/57473

公开(公告)号：EP0201633A3

公开(公告)日：1989-02-22

申请号：EP85116062.2

申请日：1985-12-17

Applicant: ABBOTT LABORATORIES

Inventor： Ungemach, Frank Schneider ,  Nystrom, David Donald ,  Keegan, Candace Linda ,  Stroupe, Stephen Denham

IPC: C07D493/10 ,  C07K13/00 ,  C07K15/00 ,  G01N33/531 ,  G01N33/94 ,  G01N21/64

CPC classification number: C07D493/10 ,  G01N33/533 ,  G01N33/9486

Abstract: The present invention is directed to a fluorescent polarization assay for acetaminophen, to the various components needed for preparing and carrying out such an assay, and to methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for making them. The tracers and the immunogens are made from substituted anilide compounds. A fluorescein moiety is included in the tracer, while a poly-(amino acid) forms a part of the immunogen. The antibodies are prepared in response to the immunogen. The assay is conducted by measuring the degree of polarization retention of plane polarized light that has been passed through a sample containing antiserum and tracer.

公开(公告)号：EP0201633A2

公开(公告)日：1986-11-20

申请号：EP85116062.2

申请日：1985-12-17

Applicant: ABBOTT LABORATORIES

Inventor： Ungemach, Frank Schneider ,  Nystrom, David Donald ,  Keegan, Candace Linda ,  Stroupe, Stephen Denham

IPC: C07D493/10 ,  C07K13/00 ,  C07K15/00 ,  G01N33/531 ,  G01N33/94 ,  G01N21/64

CPC classification number: C07D493/10 ,  G01N33/533 ,  G01N33/9486

Abstract: The present invention is directed to a fluorescent polarization assay for acetaminophen, to the various components needed for preparing and carrying out such an assay, and to methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for making them. The tracers and the immunogens are made from substituted anilide compounds. A fluorescein moiety is included in the tracer, while a poly-(amino acid) forms a part of the immunogen. The antibodies are prepared in response to the immunogen. The assay is conducted by measuring the degree of polarization retention of plane polarized light that has been passed through a sample containing antiserum and tracer.

Abstract translation: 本发明涉及对乙酰氨基酚的荧光偏振测定，制备和进行这种测定所需的各种组分以及制备这些组分的方法。 具体地，公开了示踪剂，免疫原和抗体，以及制备它们的方法。 示踪剂和免疫原由取代的酰苯胺化合物制成。 荧光素部分包含在示踪剂中，而聚（氨基酸）形成免疫原的一部分。 针对免疫原制备抗体。 该测定通过测量已经通过含有抗血清和示踪剂的样品的平面偏振光的偏振保持程度来进行。

公开(公告)号：EP0326100B1

公开(公告)日：1996-09-11

申请号：EP89101263.5

申请日：1989-01-25

Applicant: ABBOTT LABORATORIES

Inventor： Hiltibran, Robert G. ,  Jou, Yi-Her ,  Kline, Steven J. ,  Schultz, Steven George ,  Stroupe, Stephen Denham

IPC: G01N33/537 ,  G01N33/543 ,  G01N33/538

CPC classification number: G01N33/538 ,  G01N33/54366 ,  G01N33/57473

公开(公告)号：EP0664451A1

公开(公告)日：1995-07-26

申请号：EP95102406.6

申请日：1989-07-31

Applicant: ABBOTT LABORATORIES

Inventor： Hiltibran, Robert G. ,  Stroupe, Stephen Denham ,  Yi-Her, Jou

IPC: G01N33/538 ,  G01N33/543

CPC classification number: C08G69/10 ,  G01N33/538 ,  G01N33/543 ,  G01N33/54366 ,  G01N33/57473

Abstract: This invention presents novel polymeric anionic molecules and novel negatively charged capture reagents comprising the reaction products of said anionic molecules and a specific binding member for use in separation techniques and assay procedures wherein said activated polymeric anionic molecule comprises a compound having the formula:

wherein n is about 10 to about 500;
z is about 1 to about 6;
W is selected from the group consisting of H⁺, Na⁺, K⁺, Li⁺, amine salts, and derivatives thereof; and
X is a reactive group or a structure having a reactive group that enables the chemical binding of said activated polymer to a specific binding member.

Abstract translation: 本发明提出新颖的聚合阴离子分子和新颖的带负电荷的捕获试剂，其包含所述阴离子分子的反应产物和用于分离技术和测定程序的特异性结合成员，其中所述活化的聚合阴离子分子包含具有下式的化合物： 其中n为约10至约500; z为约1至约6; W选自H +，Na +，K +，Li +，胺盐及其衍生物; 并且X是具有能够使所述活化聚合物与特定结合构件化学结合的反应性基团的反应性基团或结构。

公开(公告)号：EP0406473A1

公开(公告)日：1991-01-09

申请号：EP89114103.8

申请日：1989-07-31

Applicant: ABBOTT LABORATORIES

Inventor： Hiltibran Robert G. ,  Stroupe, Stephen Denham ,  Jou, Yi-Her

IPC: G01N33/538 ,  G01N33/543

CPC classification number: C08G69/10 ,  G01N33/538 ,  G01N33/543 ,  G01N33/54366 ,  G01N33/57473

Abstract: This invention presents novel reagents, separation techniques and assay procedures which allow both the indicator and the capture reagents to be in solution to avoid problems of slowed immunoreaction kinetics. The separation procedure involves a soluble capture reagent, comprising a specific binding member attached to a charged substance, and an insoluble solid phase that is oppositely charged with respect to the capture reagent. A test sample suspected of containing the analyte of interest is mixed with the capture reagent to form a charged capture reagent/analyte complex. The reaction mixture is contacted to the oppositely charged solid phase to attract, attach, and separate the capture reagent/analyte complex from the reaction mixture. With an appropriate indicator reagent, i.e., a second specific binding substance which is conjugated to a label capable of producing a detectable signal, both sandwich and competitive assays can be performed.

Abstract translation: 本发明提出新型试剂，分离技术和测定方法，其允许指示剂和捕获试剂在溶液中以避免减慢的免疫反应动力学的问题。 分离方法包括可溶性捕获试剂，其包含与带电物质连接的特异性结合成员和相对于捕获试剂相反带电的不溶性固相。 将怀疑含有感兴趣的分析物的测试样品与捕获试剂混合以形成带电的捕获试剂/分析物复合物。 将反应混合物与相反电荷的固相接触以吸引，附着并将捕获试剂/分析物复合物与反应混合物分离。 使用合适的指示剂，即与能够产生可检测信号的标签缀合的第二特异性结合物质，可以进行夹心和竞争性测定。

公开(公告)号：EP0326100A3

公开(公告)日：1990-11-07

申请号：EP89101263.5

申请日：1989-01-25

Applicant: ABBOTT LABORATORIES

Inventor： Hiltibran, Robert G. ,  Jou, Yi-Her ,  Kline, Steven J. ,  Schultz, Steven George ,  Stroupe, Stephen Denham

IPC: G01N33/537 ,  G01N33/543 ,  G01N33/538

CPC classification number: G01N33/538 ,  G01N33/54366 ,  G01N33/57473

Abstract: This invention presents novel separation and assay procedures which allows both the indicator and the capture reagents to be in solution to avoid problems of slowed immunoreaction kinetics. The separation procedure involves an analyte-specific soluble capture reagent, that is conjugated to a charged substance, and an insoluble solid phase material that is oppositely charged. A fluid sample suspected of containing the analyte is mixed with the capture reagent in solution to form a charged capture reagent/analyte complex. When binding is complete, the solution is contacted to the oppositely charged solid phase material to attract, attach, and separate the capture reagent/analyte complex from the fluid sample. With the appropriate indicator reagent, i.e., a second analyte-­specific binding substance which is conjugated to a label capable of producing a detectable signal, both sandwich and competitive assays can be performed. The assay reaction complex can be separated from the solution by contact with the oppositely charged solid phase material, and the presence or amount of analyte is monitored by detecting the label of the indicator reagent.