公开(公告)号：EP3071951A4

公开(公告)日：2017-08-23

申请号：EP14863825

申请日：2014-11-19

Applicant: ACEA BIOSCIENCES INC

Inventor： LI NAN ,  CHEN YE ,  CHUNG HYUNSUN ,  LI ZENGQIANG ,  WANG XIAOBO

IPC: G01N21/64 ,  G01N15/10 ,  G01N15/14

CPC classification number: G01N21/645 ,  G01N15/1434 ,  G01N15/1459 ,  G01N21/6486 ,  G01N33/56972 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1438 ,  G01N2015/1477 ,  G01N2021/6471 ,  G01N2021/6478 ,  G01N2201/06113

Abstract: An optical engine for use in a bench top flow cytometer, the optical engine comprising a set of lasers; a different set of beam shaping optics for each laser, wherein each set comprises two lenses to adjustably focus light horizontally along an x-axis to a same horizontal position and vertically along a y-axis to a different vertical position along a same plane; collection optics for collecting fluorescence from the flow cell; filtration optics that filter the collected fluorescence from the flow cell into different detection channels according to wavelength ranges; and a detector for each detection channel that converts the filtered fluorescence to electrical signals, wherein electrical signals are processed so that the fluorescence from each laser at the different vertical positions is distinguished at the same detector.

Abstract translation: 一种用于台式流式细胞仪的光学引擎，该光学引擎包括一组激光器; 用于每个激光器的不同组光束成形光学器件，其中每个组包括两个透镜，以沿着x轴将水平可调地聚焦到相同的水平位置，并且沿着y轴垂直地沿着同一平面聚焦到不同的垂直位置; 用于从流动池收集荧光的收集光学器件; 过滤光学器件，其根据波长范围将从流动池收集的荧光过滤到不同的检测通道中; 以及用于每个检测通道的检测器，用于将滤波的荧光转换为电信号，其中处理电信号以使得来自不同垂直位置处的每个激光器的荧光在同一检测器处被区分。