公开(公告)号：EP2639586A1

公开(公告)日：2013-09-18

申请号：EP13159623.1

申请日：2013-03-15

申请人： ARKRAY, Inc. ,  Kikkoman Biochemifa Company

发明人： Sugiyama, Koji ,  Yonehara, Satoshi ,  Shimoji, Kazuhiko

IPC分类号： G01N33/72 ,  C12Q1/54 ,  G01N33/68 ,  G01N33/52 ,  G01N33/92

CPC分类号： C12Q1/37 ,  C12Q1/54 ,  G01N33/526 ,  G01N33/683 ,  G01N33/723 ,  G01N33/92

摘要： A method for measuring an analyte is described that includes the steps of i) preparing a reagent (D) in which an enzyme (A) and an enzyme (B) coexist in the absence of the analyte; ii) bringing the analyte into contact with the enzyme (A) and the enzyme (B) so that the enzyme (A) acts on the analyte to produce a product (E), on which the enzyme (B) does not substantially act, from the analyte; iii) producing a product (C) by allowing the enzyme (A) or an enzyme (F) that is different from the enzyme (A) to act on the analyte and/or the product (E); and iv) detecting the product (C) by the enzyme (B).

摘要翻译： 描述了测量分析物的方法，其包括以下步骤：i）制备其中酶（A）和酶（B）在不存在分析物的情况下共存的试剂（D）; ii）使分析物与酶（A）和酶（B）接触，使得酶（A）作用于分析物以产生酶（B）基本上不起作用的产物（E） 来自分析物; iii）通过使酶（A）或不同于酶（A）的酶（F）作用于分析物和/或产物（E）来产生产物（C）; 和iv）通过酶（B）检测产物（C）。

公开(公告)号：EP2336351A2

公开(公告)日：2011-06-22

申请号：EP10010191.4

申请日：2001-07-12

申请人： Arkray, Inc.

发明人： Yonehara, Satoshi

IPC分类号： C12Q1/26 ,  G01N33/72

CPC分类号： C12Q1/26 ,  C12Q1/37 ,  G01N33/723 ,  G01N33/725

摘要： A method of determining glycated hemoglobin is provided, by which a ratio of the glycated hemoglobin in a sample can be determined accurately and easily. The ratio of glycated hemoglobin can be determined by degrading a glycated hemoglobin in a whole blood sample selectively with a protease to give a glycated hemoglobin degradation product; causing a redox reaction between a glycation site of the glycated hemoglobin degradation product and a fructosyl amino acid oxidoreductase; and determining this redox reaction. Further, as shown in FIG. 1 , in a whole blood sample, there is a correlation between the ratio of the glycated hemoglobin determined by this method and an HbA1c concentration. Thus, without determining the glycated α-amino group as a characteristic structure of HbA1c, an amount of HbA1c can be determined accurately and easily from the determined ratio of the glycated hemoglobin.

摘要翻译： 提供了测定糖化血红蛋白的方法，通过该方法可以准确且容易地测定样品中糖化血红蛋白的比例。 糖化血红蛋白的比例可以通过用蛋白酶选择性降解全血样品中的糖化血红蛋白以得到糖化血红蛋白降解产物来测定; 引起糖化血红蛋白降解产物的糖化位点与果糖基氨基酸氧化还原酶之间的氧化还原反应; 并确定这种氧化还原反应。 此外，如图1所示。 如图1所示，在全血样品中，通过该方法测定的糖化血红蛋白的比例与HbA1c浓度存在相关性。 因此，在不确定糖化的±氨基作为HbA1c的特征结构的情况下，可以从确定的糖化血红蛋白的比例准确，容易地测定HbA1c的量。

公开(公告)号：EP2216413A3

公开(公告)日：2010-10-06

申请号：EP10005386.7

申请日：2002-10-09

申请人： Arkray, Inc.

发明人： Yonehara, Satoshi ,  Komori, Tsuguki

IPC分类号： C12Q1/26 ,  C12Q1/37 ,  C12Q1/28 ,  G01N33/48 ,  G01N33/50 ,  G01N33/72

CPC分类号： C12Q1/26 ,  C12Q1/37 ,  G01N33/723 ,  G01N2333/906

摘要： The present invention provides a method of pretreating a sample containing a glycated amine as an analyte, thereby enabling highly reliable measurement of a glycated amine. A glycated amino acid in the sample is degraded by causing a fructosyl amino acid oxidase (FAOD) to act thereon, and thereafter, a FAOD further is caused to act on the glycated amine as the analyte in the sample to cause a redox reaction. The amount of the glycated amine is determined by measuring the redox reaction. The substrate specificity of the FAOD caused to act on the glycated amino acid may be either the same as or different from that of the FAOD caused to act on the glycated amine. When using the same FAOD, a FAOD is caused to act on the glycated amino acid to degrade it, and thereafter, the sample is treated with a protease to inactivate the FAOD and also to degrade the glycated amine. Then, the same FAOD further is added so that the FAOD acts on the degradation product obtained to cause a redox reaction, and the redox reaction is measured.

公开(公告)号：EP2639586B1

公开(公告)日：2016-06-29

申请号：EP13159623.1

申请日：2013-03-15

申请人： ARKRAY, Inc. ,  Kikkoman Biochemifa Company

发明人： Sugiyama, Koji ,  Yonehara, Satoshi ,  Shimoji, Kazuhiko

IPC分类号： G01N33/72 ,  C12Q1/54 ,  G01N33/68 ,  G01N33/52 ,  G01N33/92

CPC分类号： C12Q1/37 ,  C12Q1/54 ,  G01N33/526 ,  G01N33/683 ,  G01N33/723 ,  G01N33/92

公开(公告)号：EP2543706A2

公开(公告)日：2013-01-09

申请号：EP12187536.3

申请日：2009-03-18

申请人： ARKRAY, Inc.

发明人： Yonehara, Satoshi ,  Inamura, Norio

IPC分类号： C09B21/00 ,  C12Q1/26 ,  C12Q1/37 ,  G01N21/78 ,  C09B11/28 ,  C09B27/06 ,  C09B29/10 ,  C09B61/00 ,  G01N31/22 ,  C09B11/08 ,  C09B29/36 ,  C09B67/22 ,  C09B67/38 ,  G01N33/53

CPC分类号： C09B21/00 ,  B82Y30/00 ,  C09B11/08 ,  C09B29/103 ,  C09B29/366 ,  C09B67/0033 ,  C09B67/0041 ,  C09B67/0089

摘要： The present invention provides a stabilizer that can stabilize a salt of 10-(carboxymethylaminocarbonyl)-3,7-bis(dimethylamino)phenothiazine or a derivative thereof even under the existence of moisture or under light irradiation. A compound described in at least one of (1) and (2) and a compound described in at least one of (3) and (4) below is used as the stabilizer of the salt of 10-(carboxymethylaminocarbonyl)-3,7-bis(dimethylamino)phenothiazine or the derivative thereof.
(1) a surfactant having an alkyl group with a carbon number of 8 to 16;
(2) at least one pigment substance selected from the group consisting of a compound represented by the following formula (I), a compound represented by the following formula (II), and a flavonoid pigment:

        R 1 -N=N-R 2      (I)



(3) quaternary ammonium having a hydrocarbon group with a carbon number of 12 or higher or a salt thereof; and
(4) β-cyclodextrin or a derivative thereof.

摘要翻译： 本发明提供了即使在水分存在或光照射下也能稳定10-（羧甲基氨基羰基）-3,7-双（二甲基氨基）吩噻嗪或其衍生物的盐的稳定剂。 使用（1）和（2）中至少之一描述的化合物和下述（3）和（4）中至少一个中所述的化合物作为10-（羧甲基氨基羰基）-3,7 双（二甲氨基）吩噻嗪或其衍生物。 （1）碳数为8〜16的烷基的表面活性剂; （2）选自由下式（I）表示的化合物，下式（II）表示的化合物和类黄酮颜料的至少一种颜料物质：€ƒ€ƒ€ƒ€ ƒ€ƒ€ƒƒR 1 -N = NR 2€ƒ€ƒ€ƒ（I）（3）碳数为12以上的烃基季铵或其盐; 和（4）β-环糊精或其衍生物。

公开(公告)号：EP2216413A2

公开(公告)日：2010-08-11

申请号：EP10005386.7

申请日：2002-10-09

申请人： Arkray, Inc.

发明人： Yonehara, Satoshi ,  Komori, Tsuguki

IPC分类号： C12Q1/26 ,  C12Q1/37

CPC分类号： C12Q1/26 ,  C12Q1/37 ,  G01N33/723 ,  G01N2333/906

摘要： The present invention provides a method of pretreating a sample containing a glycated amine as an analyte, thereby enabling highly reliable measurement of a glycated amine. A glycated amino acid in the sample is degraded by causing a fructosyl amino acid oxidase (FAOD) to act thereon, and thereafter, a FAOD further is caused to act on the glycated amine as the analyte in the sample to cause a redox reaction. The amount of the glycated amine is determined by measuring the redox reaction. The substrate specificity of the FAOD caused to act on the glycated amino acid may be either the same as or different from that of the FAOD caused to act on the glycated amine. When using the same FAOD, a FAOD is caused to act on the glycated amino acid to degrade it, and thereafter, the sample is treated with a protease to inactivate the FAOD and also to degrade the glycated amine. Then, the same FAOD further is added so that the FAOD acts on the degradation product obtained to cause a redox reaction, and the redox reaction is measured.

摘要翻译： 本发明提供预处理含糖化胺作为分析物的样品，从而使糖化胺的高度可靠的测量的方法。 样品中的糖化氨基酸，通过使果糖基氨基酸氧化酶（FAOD）以在其上作用降解，之后，一个FAOD进一步被作用于糖化胺作为样品中的分析物，以使氧化还原反应。 所述糖化胺的量确定的，通过测量氧化还原反应开采。 引起作用于糖化氨基酸的FAOD的底物特异性可以是相同，或从不同的FAOD没有的作用于糖化胺。 当使用相同的FAOD，一个用FAOD作用于糖化氨基酸上降解它，并且此后，样品被处理用蛋白酶灭活FAOD并因此分解糖化胺。 然后，将相同的FAOD进一步加入所以做了FAOD作用于获得以引起氧化还原反应的降解产物，和氧化还原反应进行测定。

公开(公告)号：EP2336351B1

公开(公告)日：2016-03-09

申请号：EP10010191.4

申请日：2001-07-12

申请人： ARKRAY, Inc.

发明人： Yonehara, Satoshi

IPC分类号： C12Q1/26 ,  G01N33/72

CPC分类号： C12Q1/26 ,  C12Q1/37 ,  G01N33/723 ,  G01N33/725

公开(公告)号：EP2325652B1

公开(公告)日：2013-10-09

申请号：EP11156454.8

申请日：2007-08-10

申请人： Arkray, Inc.

发明人： Yonehara, Satoshi ,  Imai, Toshihiro

IPC分类号： G01N33/72 ,  C07K14/805 ,  C12Q1/26 ,  C12Q1/37 ,  G01N30/88

CPC分类号： G01N33/723 ,  G01N27/447 ,  G01N2030/027 ,  G01N2333/906 ,  G01N2800/042

公开(公告)号：EP2336351A3

公开(公告)日：2011-09-14

申请号：EP10010191.4

申请日：2001-07-12

申请人： Arkray, Inc.

发明人： Yonehara, Satoshi

IPC分类号： C12Q1/26 ,  G01N33/72

CPC分类号： C12Q1/26 ,  C12Q1/37 ,  G01N33/723 ,  G01N33/725

摘要： A method of determining glycated hemoglobin is provided, by which a ratio of the glycated hemoglobin in a sample can be determined accurately and easily. The ratio of glycated hemoglobin can be determined by degrading a glycated hemoglobin in a whole blood sample selectively with a protease to give a glycated hemoglobin degradation product; causing a redox reaction between a glycation site of the glycated hemoglobin degradation product and a fructosyl amino acid oxidoreductase; and determining this redox reaction. Further, as shown in FIG. 1, in a whole blood sample, there is a correlation between the ratio of the glycated hemoglobin determined by this method and an HbA1c concentration. Thus, without determining the glycated α-amino group as a characteristic structure of HbA1c, an amount of HbA1c can be determined accurately and easily from the determined ratio of the glycated hemoglobin.

公开(公告)号：EP2325652A3

公开(公告)日：2011-08-31

申请号：EP11156454.8

申请日：2007-08-10

申请人： Arkray, Inc.

发明人： Yonehara, Satoshi ,  Imai, Toshihiro

IPC分类号： G01N33/72 ,  C07K14/805 ,  C12Q1/26 ,  C12Q1/37 ,  G01N30/88

CPC分类号： G01N33/723 ,  G01N27/447 ,  G01N2030/027 ,  G01N2333/906 ,  G01N2800/042

摘要： This invention provides a method of diagnosing postprandial hyperglycemia of a specimen which comprises measuring a postprandial hyperglycemia marker comprising hemoglobin having a glycated lysine residue by measuring a glycation degree of a lysine residue of hemoglobin with respect to a blood sample obtained from the specimen.