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公开(公告)号：EP2132296A2
公开(公告)日：2009-12-16
申请号：EP08745545.7
申请日：2008-04-10
IPC分类号： C12M3/00
CPC分类号： B01L3/502761 , B01F13/0071 , B01F13/0076 , B01L3/502792 , B01L2200/0605 , B01L2200/0647 , B01L2200/0668 , B01L2200/16 , B01L2300/0819 , B01L2300/0864 , B01L2300/0867 , B01L2300/088 , B01L2300/089 , B01L2400/0427 , B01L2400/043 , G01N27/447
摘要： The invention provides nonlimiting examples of structures for and methods of dispensing droplets in a droplet actuator. The droplet actuator structures and methods of the invention exhibit numerous advantages over droplet actuators of the prior art. In various embodiments, the structures and methods of the invention provide, among other things, improved efficiency, throughput, scalability, and/or droplet uniformity, as compared with existing droplet actuators. Further, in some embodiments, the droplet actuators provide configurations for improved methods of loading and/or unloading fluid and/or droplets. In yet other embodiments, the droplet actuators provide fluid loading configurations for loading numerous fluid reservoirs in a substantially simultaneous and/or substantially sequential manner.
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公开(公告)号：EP2279405A2
公开(公告)日：2011-02-02
申请号：EP09747457.1
申请日：2009-05-13
发明人： POLLACK, Michael , HUA, Zhishan , ECKHARDT, Allen , THWAR, Prasanna , SRINIVASAN, Vijay , PAMULA, Vamsee
CPC分类号： B01L3/502792 , B01L3/502753 , B01L3/502761 , B01L7/525 , B01L2200/10 , B01L2300/0816 , B01L2300/089 , B01L2400/0427 , B01L2400/043 , C12Q1/6848 , C12Q2565/629
摘要： The invention relates to certain novel approaches to reducing or eliminating the movement of contaminants from one droplet to another on a droplet actuator via liquid filler fluid. In one application, droplet actuators are used to conduct genetic analysis using polymerase chain reaction (PCR) techniques. The invention addresses the need for improved methods of performing PCR on a droplet actuator that provide for optimum amplification and detection of a sample target.
摘要翻译： 本发明涉及用于减少或消除液滴填充流体在液滴致动器上从一个液滴向另一个液滴移动污染物的某些新方法。 在一个应用中，液滴致动器被用于使用聚合酶链式反应（PCR）技术进行遗传分析。 本发明解决了对液滴致动器进行PCR的改进方法的需求，其提供了对样品目标的最佳放大和检测。
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3.发明公开DROPLET ACTUATOR CONFIGURATIONS AND METHODS OF CONDUCTING DROPLET OPERATIONS 审中-公开液滴驱动器配置和执行液滴操作的方法
公开(公告)号：EP2237955A2
公开(公告)日：2010-10-13
申请号：EP08868761.1
申请日：2008-12-23
发明人： SRINIVASAN, Vijay , POLLACK, Michael , PAMULA, Vamsee , HUA, Zhishan , SUDARSAN, Arjun , PAIK, Philip
CPC分类号： B01L3/52 , B01F13/0071 , B01F13/0076 , B01L3/0241 , B01L3/502792 , B01L2200/027 , B01L2200/0605 , B01L2200/143 , B01L2200/16 , B01L2300/0816 , B01L2300/0819 , B01L2300/089 , B01L2400/0427 , B01L2400/06 , B01L2400/0688 , F04B19/006
摘要： A droplet actuator with a droplet formation electrode configuration associated with a droplet operations surface, wherein the electrode configuration comprises one or more electrodes configured to control volume of a droplet during formation of a sub-droplet on the droplet operations surface. Methods of making and using the droplet actuator are also provided.
摘要翻译： 具有与液滴操作表面相关联的液滴形成电极配置的液滴致动器，其中该电极配置包括一个或多个电极，该一个或多个电极被配置为在液滴操作表面上形成子液滴期间控制液滴的体积。 还提供了制造和使用液滴致动器的方法。
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公开(公告)号：EP2212683A2
公开(公告)日：2010-08-04
申请号：EP08840456.1
申请日：2008-10-17
CPC分类号： B01L3/502792 , B01J2219/00466 , B01J2219/005 , B01J2219/00596 , B01J2219/00648 , B01J2219/00655 , B01J2219/00659 , B01L2200/0647 , B01L2200/0668 , B01L2300/0816 , B01L2300/089 , B01L2400/043
摘要： The invention provides a method of dispersing or circulating magnetically responsive beads within a droplet in a droplet actuator. The invention, in one embodiment, makes use of a droplet actuator with a plurality of droplet operations electrodes configured to transport the droplet, and a magnet field present at a portion of the plurality of droplet operations electrodes. A bead bead-containing droplet is provided on the droplet actuator in the presence of the uniform magnetic field. Beads are circulated in the droplet during incubation by conducting droplet operations on the droplet within a uniform region of the magnetic field. Other embodiments are also provided.
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公开(公告)号：EP3193180A1
公开(公告)日：2017-07-19
申请号：EP17158606.8
申请日：2011-11-15
CPC分类号： G01N27/221 , B01L3/502784 , B01L3/502792 , B01L2200/14 , B01L2300/0645 , B01L2300/0816 , B01L2300/089 , B01L2400/0427 , B41J2/04541 , B41J2/04555 , B41J2/0458 , G01K7/34
摘要： A method is described for analyzing at least one condition present in a droplet actuator (1100). The droplet actuator comprises a bottom substrate (1110) separated from a top substrate (1112) by a gap (1114). The bottom substrate includes an arrangement of droplet operations electrodes (1116). Droplet operations are conducted on droplets (1124) located atop droplet operations electrodes (1116). The gap is filed with an oil filler fluid (1122) and an impedance sensing system (1200) is electrically connected to the droplet actuator (1100). The method comprises correlating impedance measurements obtained using the impedance sensing system with at least one condition present in the droplet actuator. The impedance sensing system may comprise an impedance spectrometer. The method may be used to determine a liquid temperature, e.g., droplet temperature, or the interfacial tension of the oil layer and/or droplets, or heat produced as a byproduct of chemical reactions.
摘要翻译： 描述了用于分析存在于液滴致动器（1100）中的至少一个状况的方法。 液滴致动器包括通过间隙（1114）与顶部衬底（1112）分离的底部衬底（1110）。 底部衬底包括液滴操作电极（1116）的布置。 液滴操作在位于液滴操作电极（1116）顶上的液滴（1124）上进行。 该间隙填充有油填充流体（1122），并且阻抗感测系统（1200）电连接到液滴致动器（1100）。 该方法包括将使用阻抗感测系统获得的阻抗测量结果与存在于液滴致动器中的至少一个条件相关联。 阻抗感测系统可以包括阻抗谱仪。 该方法可用于确定液体温度，例如液滴温度，或油层和/或液滴的界面张力或作为化学反应副产物产生的热量。
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公开(公告)号：EP2672260A1
公开(公告)日：2013-12-11
申请号：EP13183436.8
申请日：2009-05-13
发明人： POLLACK, Michael , HUA, Zhishan , ECKHARDT, Allen , THWAR, Prasanna , SRINIVASAN, Vijay , PAMULA, Vamsee
CPC分类号： B01L3/502792 , B01L3/502753 , B01L3/502761 , B01L7/525 , B01L2200/10 , B01L2300/0816 , B01L2300/089 , B01L2400/0427 , B01L2400/043 , C12Q1/6848 , C12Q2565/629
摘要： The invention relates to certain novel approaches to reducing or eliminating the movement of contaminants from one droplet to another on a droplet actuator via liquid filler fluid. In one application, droplet actuators are used to conduct genetic analysis using polymerase chain reaction (PCR) techniques. The invention addresses the need for improved methods of performing PCR on a droplet actuator that provide for optimum amplification and detection of a sample target.
摘要翻译： 本发明涉及用于减少或消除液滴填充流体在液滴致动器上从一个液滴向另一个液滴移动污染物的某些新方法。 在一个应用中，液滴致动器被用于使用聚合酶链式反应（PCR）技术进行遗传分析。 本发明解决了对液滴致动器进行PCR的改进方法的需求，其提供了对样品目标的最佳放大和检测。
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公开(公告)号：EP2672259A1
公开(公告)日：2013-12-11
申请号：EP13183435.0
申请日：2009-05-13
发明人： POLLACK, Michael , HUA, Zhishan , ECKHARDT, Allen , THWAR, Prasanna , SRINIVASAN, Vijay , PAMULA, Vamsee
CPC分类号： B01L3/502792 , B01L3/502753 , B01L3/502761 , B01L7/525 , B01L2200/10 , B01L2300/0816 , B01L2300/089 , B01L2400/0427 , B01L2400/043 , C12Q1/6848 , C12Q2565/629
摘要： The invention relates to certain novel approaches to reducing or eliminating the movement of contaminants from one droplet to another on a droplet actuator via liquid filler fluid. In one application, droplet actuators are used to conduct genetic analysis using polymerase chain reaction (PCR) techniques. The invention addresses the need for improved methods of performing PCR on a droplet actuator that provide for optimum amplification and detection of a sample target.
摘要翻译： 本发明涉及通过液体填充液减少或消除液滴致动器上的污染物从一个液滴到另一个液滴的移动的某些新颖方法。 在一个应用中，液滴致动器用于使用聚合酶链反应（PCR）技术进行遗传分析。 本发明解决了在提供对样品靶的最佳放大和检测的液滴致动器上进行PCR的改进方法的需要。
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公开(公告)号：EP2279405B1
公开(公告)日：2013-09-18
申请号：EP09747457.1
申请日：2009-05-13
发明人： POLLACK, Michael , HUA, Zhishan , ECKHARDT, Allen , THWAR, Prasanna , SRINIVASAN, Vijay , PAMULA, Vamsee
CPC分类号： B01L3/502792 , B01L3/502753 , B01L3/502761 , B01L7/525 , B01L2200/10 , B01L2300/0816 , B01L2300/089 , B01L2400/0427 , B01L2400/043 , C12Q1/6848 , C12Q2565/629
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公开(公告)号：EP2705374A2
公开(公告)日：2014-03-12
申请号：EP12779928.6
申请日：2012-05-01
发明人： BORT, Donovan , GRAHAM, Carrie , PAMULA, Vamsee , POLLACK, Michael , SISTA, Ramakrishna , SRINIVASAN, Vijay
CPC分类号： G01N27/44791 , B01F1/0027 , B01F13/0071 , B01F13/0076 , B01F2001/0055 , B01F2215/0037 , B01L3/502761 , B01L3/502792 , B01L2200/0605 , B01L2200/0668 , B01L2200/10 , B01L2200/16 , B01L2300/0816 , B01L2300/0867 , B01L2300/089 , B01L2400/0406 , B01L2400/0418 , B01L2400/0427 , B01L2400/043 , B01L2400/0487 , B81B1/004
摘要： A droplet actuator (110) comprising: one or more substrates (114, 116) arranged to form a droplet operations gap, the droplet operations gap comprising a region of gap height transition in which the gap height of the droplet operations gap transitions from a first gap height to a second gap height; and electrodes associated with the one or more substrates arranged for conducting droplet operations, the electrodes comprising a transition electrode which traverses the region of gap height transition. The electrodes may comprise electrowetting electrodes. The transition electrode may be flanked by first and second sets of electrodes having first and second footprint areas. The first footprint area may be greater than the second footprint area. The first set of electrodes may be arranged in a region of greater gap height and the second set of electrodes may be arranged in the region of lesser gap height.
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公开(公告)号：EP2641097A2
公开(公告)日：2013-09-25
申请号：EP11841725.2
申请日：2011-11-15
IPC分类号： G01R27/26
CPC分类号： G01N27/221 , B01L3/502784 , B01L3/502792 , B01L2200/14 , B01L2300/0645 , B01L2300/0816 , B01L2300/089 , B01L2400/0427 , B41J2/04541 , B41J2/04555 , B41J2/0458 , G01K7/34
摘要： The invention provides for a method of performing capacitance detection on a droplet actuator. A capacitor may be formed by the combination of a conductive droplet, an insulator layer, and one or more transport electrodes within a droplet actuator. At any given electrode, the capacitance measured is proportional to the footprint area of a droplet thereon. Capacitance detection methods of the invention herein may be used as a real-time verification tool in order to detect the absence, presence, and/or partial presence of a droplet at an electrode; analysis of droplet properties; measurement of droplet size or volume; optimization of the speed of droplet operations; and detection of air bubbles. The method may include applying a base oscillation frequency at the position and detecting a deviation from the base oscillation frequency.
摘要翻译： 本发明提供了一种在液滴致动器上执行电容检测的方法。 电容器可以通过导电液滴，绝缘体层和液滴致动器内的一个或多个传输电极的组合来形成。 在任何给定的电极上，所测量的电容与其上的液滴的占地面积成比例。 本文中的本发明的电容检测方法可以用作实时验证工具以检测电极处液滴的存在，存在和/或部分存在; 液滴性质分析; 测量液滴尺寸或体积; 液滴操作速度的优化; 并检测气泡。 该方法可以包括在该位置施加基本振荡频率并且检测与基本振荡频率的偏差。
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