公开(公告)号：EP2212683A4

公开(公告)日：2011-08-31

申请号：EP08840456

申请日：2008-10-17

申请人： ADVANCED LIQUID LOGIC INC ,  ECKHARDT ALLEN

发明人： SISTA RAMAKRISHNAN ,  PAMULA VAMSEE ,  SRINIVASAN VIJAY ,  POLLACK MICHAEL

IPC分类号： G01N27/26 ,  B01J19/08

CPC分类号： B01L3/502792 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00655 ,  B01J2219/00659 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/089 ,  B01L2400/043

公开(公告)号：EP2212683A2

公开(公告)日：2010-08-04

申请号：EP08840456.1

申请日：2008-10-17

申请人： Advanced Liquid Logic, Inc. ,  Eckhardt, Allen

发明人： SISTA, Ramakrishnan ,  PAMULA, Vamsee ,  SRINIVASAN, Vijay ,  POLLACK, Michael

IPC分类号： G01N27/26 ,  B01J19/08

CPC分类号： B01L3/502792 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00655 ,  B01J2219/00659 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/089 ,  B01L2400/043

摘要： The invention provides a method of dispersing or circulating magnetically responsive beads within a droplet in a droplet actuator. The invention, in one embodiment, makes use of a droplet actuator with a plurality of droplet operations electrodes configured to transport the droplet, and a magnet field present at a portion of the plurality of droplet operations electrodes. A bead bead-containing droplet is provided on the droplet actuator in the presence of the uniform magnetic field. Beads are circulated in the droplet during incubation by conducting droplet operations on the droplet within a uniform region of the magnetic field. Other embodiments are also provided.

公开(公告)号：EP2126038A4

公开(公告)日：2010-11-24

申请号：EP08732728

申请日：2008-03-23

申请人： ADVANCED LIQUID LOGIC INC ,  PAMULA VAMSEE ,  ECKHARDT ALLEN

发明人： PAMULA VAMSEE ,  ECKHARDT ALLEN

IPC分类号： C12M1/22 ,  B01F13/00 ,  C12M3/00 ,  C12Q1/34

CPC分类号： G01N21/6486 ,  B01F13/0809 ,  C07H15/04 ,  C12Q1/34 ,  G01N2333/928 ,  G01N2800/042

公开(公告)号：EP2126038A1

公开(公告)日：2009-12-02

申请号：EP08732728.4

申请日：2008-03-23

申请人： Advanced Liquid Logic, Inc. ,  Pamula, Vamsee ,  Eckhardt, Allen

发明人： PAMULA, Vamsee ,  ECKHARDT, Allen

IPC分类号： C12M1/22 ,  C12M3/00

CPC分类号： G01N21/6486 ,  B01F13/0809 ,  C07H15/04 ,  C12Q1/34 ,  G01N2333/928 ,  G01N2800/042

摘要： A method of conducting a droplet-based enzymatic assay is provided. The method generally makes use of a droplet actuator. A droplet comprising an enzyme of interest is provided on the droplet actuator along with a droplet comprising a substrate which is potentially modified in the presence of the enzyme. The method involves executing droplet operations on the droplet actuator to combine the droplets, thereby yielding an assay droplet, and detecting modification of the substrate by the enzyme in the assay droplet on the droplet actuator. The enzyme of interest may, for example, be a potentially mutated or improperly folded enzyme exhibiting altered enzyme activity as compared to a corresponding normal enzyme.

摘要翻译： 提供了进行基于液滴的酶测定的方法。 该方法通常使用液滴致动器。 包含感兴趣的酶的液滴与包含可能在酶存在下被修饰的底物的液滴一起提供在液滴致动器上。 该方法包括在液滴致动器上执行液滴操作以组合液滴，由此产生测定液滴，并且检测液滴致动器上的测定液滴中的酶对底物的修饰。 感兴趣的酶可以是例如与相应的正常酶相比呈现改变的酶活性的潜在突变或不适当折叠的酶。