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    TEMPERATURE SENSITIVE dtsR GENES
    4.
    发明公开
    TEMPERATURE SENSITIVE dtsR GENES 失效
    TEMPERATUR-EMPFINDLICHE dtsR基因

    公开(公告)号：EP1002866A1

    公开(公告)日：2000-05-24

    申请号：EP98929834.4

    申请日：1998-07-03

    申请人： Ajinomoto Co., Inc.

    发明人： KIMURA, Eiichiro, Ajinomoto Co., Inc. ,  YAGOSHI, Chizu, Ajinomoto Co., Inc. ,  NAKAMURA, Jun, Ajinomoto Co., Inc. ,  OSUMI, Tsuyoshi, Ajinomoto Co., Inc. ,  NAKAMATSU, Tsuyoshi, Ajinomoto Co., Inc.

    IPC分类号： C12N15/31 ,  C12N1/21 ,  C12P13/08 ,  C12P13/14

    CPC分类号： C07K14/345 ,  C12P13/08 ,  C12P13/14

    摘要： DNAS encoding the following proteins (A) or (B) which participate in the temperature sensitivity to surfactants of corynebacteria: (A) a protein having the amino acid sequence represented by SEQ ID NO:2 or a protein having the amino acid sequence represented by SEQ ID NO:2 wherein Leu at the 139-position is substituted by another amino acid residue excluding Pro and having a temperature sensitive DTSR activity; and (B) proteins derived from the above proteins (A) by substitution, deletion, insertion, addition or inversion of one or several amino acid residues except the one at the 139-position in the amino acid sequence represented by SEQ ID NO:2 and having the temperature sensitive DTSR activity.

    摘要翻译： 编码参与对棒状杆菌表面活性剂的温度敏感性的以下蛋白质（A）或（B）的DNAS：（A）具有SEQ ID NO：2所示的氨基酸序列的蛋白质或具有SEQ ID NO：2所示的氨基酸序列的蛋白质 SEQ ID NO：2，其中在139位的Leu被除了Pro之外的另一个氨基酸残基取代并且具有温度敏感的DTSR活性; 和（B）衍生自上述蛋白质（A）的蛋白质，除了SEQ ID NO：2所示的氨基酸序列中139位以外的一个或几个氨基酸残基的取代，缺失，插入，添加或倒位 并具有温度敏感的DTSR活性。

    PROCESS FOR PRODUCING TARGET SUBSTANCES BY FERMENTATION
    6.
    发明公开
    PROCESS FOR PRODUCING TARGET SUBSTANCES BY FERMENTATION 失效
    VERFAHREN ZUR HERSTELLUNG VON ZIELSUBSTANZEN DURCH FERMENTATION

    公开(公告)号：EP0974647A1

    公开(公告)日：2000-01-26

    申请号：EP97924309.4

    申请日：1997-06-04

    申请人： Ajinomoto Co., Inc.

    发明人： KUWABARA, Yoko, Ajinomoto Co., Inc. ,  KIMURA, Eiichiro, Ajinomoto Co., Inc. ,  KAWAHARA, Yoshio, Ajinomoto Co., Inc. ,  NAKAMATSU, Tsuyoshi, Ajinomoto Co., Inc.

    IPC分类号： C12N1/21 ,  C12P13/00

    CPC分类号： C12N15/69

    摘要： An object resides in controlling the retaining and dissociation of a gene extrachromosomally for efficient production of an objective substance in a fermentative manner.
    By culturing and growing a microorganism containing a plasmid carrying a gene disadvantageously functioning for the production of an objective enzyme and a temperature-sensitive replication origin, on which plasmid the functional gene is solely present, at a temperature at which the plasmid is replicable, and continuously culturing the microorganism at a temperature at which the plasmid is never replicable, to dissociate the plasmid from the cells and continue the culturing, the objective substance can efficiently be produced.

    摘要翻译： 一个目的在于控制染色体外基因的保留和解离以有效地以发酵方式生产目标物质。 通过在质粒可复制的温度下培养和生长含有携带不利地用于产生目标酶和温度敏感性复制起点的基因的质粒，其中仅存在功能基因的质粒， 在质粒永不复制的温度下连续培养微生物，从细胞中分离质粒并继续培养，可有效地制备目标物质。