公开(公告)号：EP3077528A1

公开(公告)日：2016-10-12

申请号：EP14806590.7

申请日：2014-12-01

申请人： Albert-Ludwigs-Universität Freiburg ,  Karlsruher Institut für Technologie

发明人： NAZARENKO, Irina ,  CATO, Andrew ,  NEEB, Antje ,  BORMANN, Stefanie ,  SCHÖNTHALER, Martin ,  MIERNIK, Arkadiusz ,  KRÖNIG, Malte ,  WILHELM, Konrad ,  HÄNSELMANN, Benjamin ,  HEFELE, Simon

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/158

摘要： The present invention relates to an in vitro process for the diagnosis of prostate cancer and other tumor types in exosomes obtained from a body fluid which comprises a) concentrating the exosomes and other extracellular vesicles from a body fluid of a patient, b) extraction of total RNA from the exosomes obtained in step a), c) conversion of the RNA obtained in step b) to cDNA, d) amplification of the cDNA obtained in step c) with a polymerase chain reaction whereby primers derived from the AGR2 nucleotide sequence or the complement thereof are used, and e) determining whether in the amplification product variants of the AGR2 gene can be identified.

公开(公告)号：EP3077528B1

公开(公告)日：2018-08-22

申请号：EP14806590.7

申请日：2014-12-01

申请人： Albert-Ludwigs-Universität Freiburg ,  Karlsruher Institut für Technologie

发明人： NAZARENKO, Irina ,  CATO, Andrew ,  NEEB, Antje ,  BORMANN, Stefanie ,  SCHÖNTHALER, Martin ,  MIERNIK, Arkadiusz ,  KRÖNIG, Malte ,  WILHELM, Konrad ,  HÄNSELMANN, Benjamin ,  HEFELE, Simon

IPC分类号： C12Q1/6886

CPC分类号： C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/158

摘要： The present invention relates to an in vitro process for the diagnosis of prostate cancer and other tumor types in exosomes obtained from a body fluid which comprises a) concentrating the exosomes and other extracellular vesicles from a body fluid of a patient, b) extraction of total RNA from the exosomes obtained in step a), c) conversion of the RNA obtained in step b) to cDNA, d) amplification of the cDNA obtained in step c) with a polymerase chain reaction whereby primers derived from the AGR2 nucleotide sequence or the complement thereof are used, and e) determining whether in the amplification product variants of the AGR2 gene can be identified.

公开(公告)号：EP3177710A1

公开(公告)日：2017-06-14

申请号：EP15745401.8

申请日：2015-07-31

申请人： Karlsruher Institut für Technologie

发明人： GRUHL, Friederike ,  GÖPPERT, Bettina ,  CATO, Andrew ,  NEEB, Antje

IPC分类号： C12N5/00

CPC分类号： C12N5/0062 ,  C12N2533/40 ,  C12N2539/00

摘要： The invention relates to a 3D cell culture system comprising a cryogel consisting of at least one polyethylene glycol diacrylate (PEGda). The invention further relates to a method for preparing a 3D cell culture system consisting of at least one polyethylene glycol diacrylate cryogel, wherein a pre-cooled mixture of polyethylene glycol diacrylate is initially provided in a buffer solution, whereupon at least one polymerization initiator and at least one polymerization catalyst are added. The mixture is frozen, whereupon the cryogel can be removed.

摘要翻译： 本发明涉及包含由至少一种聚乙二醇二丙烯酸酯（PEGda）组成的冷冻凝胶的3D细胞培养系统。 本发明进一步涉及用于制备由至少一种聚乙二醇二丙烯酸酯cryogel组成的三维细胞培养体系的方法，其中将聚乙二醇二丙烯酸酯的预冷混合物初始提供在缓冲溶液中，于是至少一种聚合引发剂和 加入至少一种聚合催化剂。 冷冻混合物，随后可除去冻凝胶。