公开(公告)号：EP1518114A1

公开(公告)日：2005-03-30

申请号：EP03733764.9

申请日：2003-06-18

申请人： Amersham Biosciences AB

发明人： Larsson, Anders ,  Meyer, Ulrika ,  Stridsberg FRID N Kajsa ,  Von HEIJNE, Eva

IPC分类号： G01N30/48 ,  G01N30/56 ,  B01J20/32 ,  B01J20/22

CPC分类号： B01J20/286 ,  B01D15/327 ,  B01D15/361 ,  B01D15/3804 ,  B01J20/26 ,  B01J20/265 ,  B01J20/285 ,  B01J20/3272 ,  B01J20/3274 ,  B01J39/26 ,  B01J41/20 ,  C08B37/00 ,  C08G81/00 ,  C08J7/12

摘要： The present invention is a surface-modified base matrix comprised of a porous polymeric base matrix onto which branched hydrophilic polyhydroxy-functional polymers have been covalently attached, wherein the polyhydroxy-functional polymers are hyperbranched polymers presenting a degree of branching (DB) of at least about 0.2 and each polymer is tethered to the base matrix at two or more points. The present matrix can for example be a cross-linked carbohydrate material, such as agarose, and the hyperbranched hydrophilic polymer can e.g. be a copolymer of epichiorohydrin and a sugar. The invention also relates to a method of surface-modification of a porous base matrix by activating functional hydroxy groups thereon and contacting the activated matrix with a hydrophilic hyperbranched hydroxy-functional polymer.