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公开(公告)号：EP2003969A2
公开(公告)日：2008-12-24
申请号：EP07755113.3
申请日：2007-04-06
CPC分类号： C12N5/0639 , A61K2039/5154 , A61K2039/5156 , A61K2039/5158 , C12N2501/24 , C12N2501/52 , C12N2510/00
摘要： This invention provides novel CD40L polypeptides and nucleic acids, as well as antigen presenting cells and vaccines comprising such CD40L polypeptides and/or nucleic acids, and related methods for preparing the antigen presenting cells and vaccines. The antigen presenting cells and vaccines are useful for enhancing an immune response. The invention provides a method for preparing mature dendritic cells (DCs), comprising the sequential steps of: (a) signaling isolated immature dendritic cells (iDCs) with a first signal comprising an interferon gamma receptor (IFN-?R) agonist and/or a tumor necrosis factor alpha receptor (TNF-?R) agonist to produce signaled dendritic cells; and (b) signaling said signaled dendritic cells with a second transient signal comprising an effective amount of a CD40 agonist to produce CCR7+ mature dendritic cells. Also provided by this invention are enriched populations of dendritic cells prepared by the methods of the invention. Such dendritic cells have enhanced immunostimulatory properties and increased IL-12 secretion and/or decreased IL-10 secretion. CD40 signaling can be initiated by one or more of polypeptide translated from an exogenous polynucleotide encoding CD40L (e.g., mRNA or DNA), an agonistic antibody to CD40 receptor or by CD40 ligand polypeptide. The enriched populations can be further modified by the administration of an immunogen to the DC. The DC will take up and process the immunogen on its cell surface.
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公开(公告)号：EP1794327A2
公开(公告)日：2007-06-13
申请号：EP05797971.8
申请日：2005-09-14
IPC分类号： C12Q1/70
CPC分类号： C07K14/005 , A61K39/21 , A61K2039/5154 , A61K2039/5156 , C12N7/00 , C12N2740/16041 , C12N2740/16234 , C12N2740/16334 , C12Q1/703
摘要： This in invention relates to methods for the nucleic acid amplification of multiple variants (strains) of any pathogen present in a sample, and preferably in a sample from a pathogen infected individual. In preferred embodiments, the pathogen is a retrovirus, such as HIV. The amplified pathogen nucleic acid can be used to identify the pathogen variants present in a sample, to quantitate the pathogen present in a sample, and as a nucleic acid vaccine, or in the preparation of antigen presenting cell vaccines. Nucleic acids produced by the methods of the invention or the proteins encoded thereby can be used to transfect/load antigen presenting cells. The loaded antigen presenting cells can then be used as a vaccine for the treatment of pathogen infection.; In another embodiment, nucleic acids produced by the methods of the invention can be used directly as nucleic acid vaccines without prior loading into antigen presenting cells.
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公开(公告)号：EP1882031B1
公开(公告)日：2017-11-08
申请号：EP06749566.3
申请日：2006-04-07
IPC分类号： C12N5/0784
CPC分类号： C12N5/0639 , A61K2039/5154 , C12N2501/02 , C12N2501/22 , C12N2501/23 , C12N2501/2301 , C12N2501/2304 , C12N2501/2306 , C12N2501/24 , C12N2501/25 , C12N2502/11 , C12N2506/11 , C12N2506/115 , C12N2523/00
摘要： Methods are provided for the production of dendritic cells from monocytes that have been incubated at a temperature of 1° C.-34° C. for a period of approximately 6 to 96 hours from the time they are isolated from a subject. After the incubation period, the monocytes can then be induced to differentiate into dendritic cells. Mature dendritic cells made by the methods of the invention have increased levels of one or more of CD80, CD83, CD86, MHC class I molecules, or MHC class II molecules as compared to mature dendritic cells prepared from monocytes that have not been held at 1° C.-34° C. for at least 6 hours from the time they were isolated from a subject. Dendritic cells made by the methods of the invention are useful for the preparation of vaccines and for the stimulation of T cells.
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公开(公告)号：EP2003969B1
公开(公告)日：2011-05-25
申请号：EP07755113.3
申请日：2007-04-06
CPC分类号： C12N5/0639 , A61K2039/5154 , A61K2039/5156 , A61K2039/5158 , C12N2501/24 , C12N2501/52 , C12N2510/00
摘要： This invention provides novel CD40L polypeptides and nucleic acids, as well as antigen presenting cells and vaccines comprising such CD40L polypeptides and/or nucleic acids, and related methods for preparing the antigen presenting cells and vaccines. The antigen presenting cells and vaccines are useful for enhancing an immune response. The invention provides a method for preparing mature dendritic cells (DCs), comprising the sequential steps of: (a) signaling isolated immature dendritic cells (iDCs) with a first signal comprising an interferon gamma receptor (IFN-?R) agonist and/or a tumor necrosis factor alpha receptor (TNF-?R) agonist to produce signaled dendritic cells; and (b) signaling said signaled dendritic cells with a second transient signal comprising an effective amount of a CD40 agonist to produce CCR7+ mature dendritic cells. Also provided by this invention are enriched populations of dendritic cells prepared by the methods of the invention. Such dendritic cells have enhanced immunostimulatory properties and increased IL-12 secretion and/or decreased IL-10 secretion. CD40 signaling can be initiated by one or more of polypeptide translated from an exogenous polynucleotide encoding CD40L (e.g., mRNA or DNA), an agonistic antibody to CD40 receptor or by CD40 ligand polypeptide. The enriched populations can be further modified by the administration of an immunogen to the DC. The DC will take up and process the immunogen on its cell surface.
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公开(公告)号：EP1882031A2
公开(公告)日：2008-01-30
申请号：EP06749566.3
申请日：2006-04-07
IPC分类号： C12N5/06
CPC分类号： C12N5/0639 , A61K2039/5154 , C12N2501/02 , C12N2501/22 , C12N2501/23 , C12N2501/2301 , C12N2501/2304 , C12N2501/2306 , C12N2501/24 , C12N2501/25 , C12N2502/11 , C12N2506/11 , C12N2506/115 , C12N2523/00
摘要： Methods are provided for the production of dendritic cells from monocytes that have been incubated at a temperature of 1° C.-34° C. for a period of approximately 6 to 96 hours from the time they are isolated from a subject. After the incubation period, the monocytes can then be induced to differentiate into dendritic cells. Mature dendritic cells made by the methods of the invention have increased levels of one or more of CD80, CD83, CD86, MHC class I molecules, or MHC class II molecules as compared to mature dendritic cells prepared from monocytes that have not been held at 1° C.-34° C. for at least 6 hours from the time they were isolated from a subject. Dendritic cells made by the methods of the invention are useful for the preparation of vaccines and for the stimulation of T cells.
摘要翻译： 提供了用于从单核细胞生产树突状细胞的方法，其已经在从受试者分离出的时间内在温度为1℃-34℃下温育约6至96小时。 潜伏期后，可诱导单核细胞分化为树突状细胞。 通过本发明的方法制备的成熟树突状细胞与从未保持在1的单核细胞制备的成熟树状细胞相比，具有增加的CD80，CD83，CD86，MHC I类分子或MHC II类分子中的一种或多种的水平 从受试者分离至少6个小时。 通过本发明方法制备的树突状细胞可用于制备疫苗和刺激T细胞。
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公开(公告)号：EP1697396A2
公开(公告)日：2006-09-06
申请号：EP04812120.6
申请日：2004-11-24
发明人： TCHEREPANOVA, Irina
CPC分类号： A61K39/00 , A61K2039/5154 , A61K2039/5156 , A61K2039/53 , C12Q1/6865 , Y02A50/407 , Y02A50/41 , Y02A50/412 , Y02A50/466 , Y02A50/48 , Y02A50/56 , C12Q2525/173
摘要： A method of amplifying RNA to obtain RNA molecules that are predominantly in the sense orientation and essentially devoid of RNA molecules that are in the anti-sense orientation. A method of transfecting antigen presenting cells with a composition comprising sense RNA encoding immunogenic antigens and essentially devoid of antisense RNA and dsRNA is also provided as well as dendritic cells prepared according to the method.
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公开(公告)号：EP1794327B1
公开(公告)日：2016-11-30
申请号：EP05797971.8
申请日：2005-09-14
CPC分类号： C07K14/005 , A61K39/21 , A61K2039/5154 , A61K2039/5156 , C12N7/00 , C12N2740/16041 , C12N2740/16234 , C12N2740/16334 , C12Q1/703
摘要： This in invention relates to methods for the nucleic acid amplification of multiple variants (strains) of any pathogen present in a sample, and preferably in a sample from a pathogen infected individual. In preferred embodiments, the pathogen is a retrovirus, such as HIV. The amplified pathogen nucleic acid can be used to identify the pathogen variants present in a sample, to quantitate the pathogen present in a sample, and as a nucleic acid vaccine, or in the preparation of antigen presenting cell vaccines. Nucleic acids produced by the methods of the invention or the proteins encoded thereby can be used to transfect/load antigen presenting cells. The loaded antigen presenting cells can then be used as a vaccine for the treatment of pathogen infection.; In another embodiment, nucleic acids produced by the methods of the invention can be used directly as nucleic acid vaccines without prior loading into antigen presenting cells.
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8.发明授权MATURE DENDRITIC CELL COMPOSITIONS AND METHODS FOR CULTURING SAME 有权ZUSAMMENSETZUNGEN REIFER DENDRITISCHER ZELLEN UND VERFAHREN ZU DEREN KULTIVIERUNG
公开(公告)号：EP1809737B9
公开(公告)日：2011-10-05
申请号：EP05804341.5
申请日：2005-10-07
IPC分类号： C12N5/0784 , A61K39/00
CPC分类号： C12N5/0639 , A61K2039/5154 , C12N15/87 , C12N2500/36 , C12N2501/02 , C12N2501/22 , C12N2501/23 , C12N2501/24 , C12N2501/52 , C12N2510/00
摘要： This invention provides methods to prepare and use immunostimulatory cells for enhancing an immune response. The invention provides a method for preparing mature dendritic cells (DCs), comprising the sequential steps of: (a) signaling isolated immature dendritic cells (iDCs) with a first signal comprising an interferon gamma receptor (IFN-³R) agonist and/or a tumor necrosis factor alpha receptor (TNF-±R) agonist to produce signaled dendritic cells; and (b) signaling said signaled dendritic cells with a second transient signal comprising an effective amount of a CD40 agonist to produce CCR7+ mature dendritic cells. Also provided by this invention are enriched populations of dendritic cells prepared by the methods of the invention. Such dendritic cells have enhanced immunostimulatory properties and increased IL-12 secretion and/or decreased IL-10 secretion. CD40 signaling can be initiated by one or more of polypeptide translated from an exogenous polynucleotide encoding CD40L (e.g. mRNA or DNA), an agonistic antibody to CD40 receptor or by CD40 ligand polypeptide. The enriched populations can be further modified by the administration of an immunogen to the DC. The DC will take up and process the immunogen on its cell surface.
摘要翻译： 本发明提供了制备和使用免疫刺激细胞增强免疫应答的方法。 本发明提供了一种制备成熟树突状细胞（DC）的方法，包括以下顺序步骤：（a）用包含干扰素γ受体（IFN-3R）激动剂和/或 肿瘤坏死因子α受体（TNF-R）激动剂产生信号传导的树突状细胞; 和（b）用包含有效量的CD40激动剂的第二瞬时信号发信号通知所述信号传导的树突状细胞以产生CCR7 +成熟树突状细胞。 本发明还提供了通过本发明的方法制备的富含树枝状细胞的群体。 这种树突状细胞具有增强的免疫刺激性能和增加的IL-12分泌和/或降低的IL-10分泌。 可以由编码CD40L（例如mRNA或DNA）的外源多核苷酸，CD40受体的激动性抗体或CD40配体多肽翻译的一种或多种多肽来启动CD40信号传导。 通过向DC施用免疫原可以进一步修饰富集的群体。 DC将占据并处理细胞表面的免疫原。
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公开(公告)号：EP1809737B1
公开(公告)日：2010-11-24
申请号：EP05804341.5
申请日：2005-10-07
IPC分类号： C12N5/0784 , A61K39/00
CPC分类号： C12N5/0639 , A61K2039/5154 , C12N15/87 , C12N2500/36 , C12N2501/02 , C12N2501/22 , C12N2501/23 , C12N2501/24 , C12N2501/52 , C12N2510/00
摘要： This invention provides methods to prepare and use immunostimulatory cells for enhancing an immune response. The invention provides a method for preparing mature dendritic cells (DCs), comprising the sequential steps of: (a) signaling isolated immature dendritic cells (iDCs) with a first signal comprising an interferon gamma receptor (IFN-³R) agonist and/or a tumor necrosis factor alpha receptor (TNF-±R) agonist to produce signaled dendritic cells; and (b) signaling said signaled dendritic cells with a second transient signal comprising an effective amount of a CD40 agonist to produce CCR7+ mature dendritic cells. Also provided by this invention are enriched populations of dendritic cells prepared by the methods of the invention. Such dendritic cells have enhanced immunostimulatory properties and increased IL-12 secretion and/or decreased IL-10 secretion. CD40 signaling can be initiated by one or more of polypeptide translated from an exogenous polynucleotide encoding CD40L (e.g. mRNA or DNA), an agonistic antibody to CD40 receptor or by CD40 ligand polypeptide. The enriched populations can be further modified by the administration of an immunogen to the DC. The DC will take up and process the immunogen on its cell surface.
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公开(公告)号：EP1809737A2
公开(公告)日：2007-07-25
申请号：EP05804341.5
申请日：2005-10-07
CPC分类号： C12N5/0639 , A61K2039/5154 , C12N15/87 , C12N2500/36 , C12N2501/02 , C12N2501/22 , C12N2501/23 , C12N2501/24 , C12N2501/52 , C12N2510/00
摘要： This invention provides methods to prepare and use immunostimulatory cells for enhancing an immune response. The invention provides a method for preparing mature dendritic cells (DCs), comprising the sequential steps of: (a) signaling isolated immature dendritic cells (iDCs) with a first signal comprising an interferon gamma receptor (IFN-³R) agonist and/or a tumor necrosis factor alpha receptor (TNF-±R) agonist to produce signaled dendritic cells; and (b) signaling said signaled dendritic cells with a second transient signal comprising an effective amount of a CD40 agonist to produce CCR7+ mature dendritic cells. Also provided by this invention are enriched populations of dendritic cells prepared by the methods of the invention. Such dendritic cells have enhanced immunostimulatory properties and increased IL-12 secretion and/or decreased IL-10 secretion. CD40 signaling can be initiated by one or more of polypeptide translated from an exogenous polynucleotide encoding CD40L (e.g. mRNA or DNA), an agonistic antibody to CD40 receptor or by CD40 ligand polypeptide. The enriched populations can be further modified by the administration of an immunogen to the DC. The DC will take up and process the immunogen on its cell surface.
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