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公开(公告)号：EP4276188A9
公开(公告)日：2025-01-15
申请号：EP21924111.4
申请日：2021-07-06
Applicant: Asymchem Laboratories (Tianjin) Co., Ltd.
Inventor： HONG, Hao , JAMES, Gage , XIAO, Yi , ZHANG, Na , JIAO, Xuecheng , MA, Yulei , MOU, Huiyan , WANG, Zujian , SUN, Kaihua , JIA, Ru , LIU, Fang , LIU, Wenjing
Abstract: Provided is a method for synthesizing a chiral amine compound. A transaminase is used to transaminate a ketone substrate under the action of an amino donor, to obtain the chiral amine compound; and the conserved amino acid sequence region of the transaminase at least includes a region 1 (MAGLWCVN) and a region 2 (YNTFFKT). With the transaminase with the specific conserved amino acid sequence region to synthesize a large sterically hindered chiral amine, the enzyme catalytic reaction volume is small, the synthesizing route is short, the product yield is high, a high-cost noble metal is not required for catalysis under the synthesizing conditions, three wastes are reduced, and the production cost is saved.
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公开(公告)号：EP4273237A1
公开(公告)日：2023-11-08
申请号：EP21924110.6
申请日：2021-07-06
Applicant: Asymchem Laboratories (Tianjin) Co., Ltd.
Inventor： HONG, Hao , JAMES, Gage , XIAO, Yi , ZHANG, Na , JIAO, Xuecheng , MA, Yulei , MOU, Huiyan , WANG, Zujian , SUN, Kaihua , LI, Xiang , ZHAO, Tong , CAO, Shan
Abstract: Provided are transaminase mutants and uses thereof. The transaminase mutant is obtained by one or more amino acid mutations occurring in SEQ ID NO: 2 or is a mutant with a conserved amino acid mutation obtained by taking the sequence SEQ ID NO: 1 of a wild-type CvTA transaminase as a reference. Compared with wild-type transaminases, the catalytic activity of the mutant is improved to different degrees, so that the production efficiency of chiral amine compound synthesis may be improved.
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公开(公告)号：EP4512891A1
公开(公告)日：2025-02-26
申请号：EP22938043.1
申请日：2022-05-17
Applicant: Asymchem Laboratories (Tianjin) Co., Ltd.
Inventor： HONG, Hao , JAMES, Gage , XIAO, Yi , ZHANG, Na , JIAO, Xuecheng , ZHANG, Kejian , WANG, Lei , ZHAO, Junqi , LING, Jun , XU, Xinsheng , LI, Rui , WANG, Zujian
IPC: C12N9/10 , C12N15/54 , C12N15/70 , C12N15/81 , C12N1/19 , C12N1/21 , C12P13/00 , C12R1/19 , C12R1/645
Abstract: Provided are a transaminase mutant and use thereof. The transaminase mutant is obtained by mutation occurred in an amino acid sequence as shown in SEQ ID NO:1, and the mutation includes at least T291A; or an amino acid sequence of the transaminase mutant has the mutation in the mutated amino acid sequence, and has more than 80% of identity with the mutated amino acid sequence, preferably has 85% of identity, and more preferably has 90% of identity. The transaminase mutant expands a substrate spectrum of enzymes, improves stereoselectivity, and improves the catalytic capacity of a large steric hindrance substrate; and at the same time, the stability of the enzymes is improved, and a catalytic reaction may be performed to produce chiral amines at high solvent concentrations and high temperatures.
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公开(公告)号：EP4276188A1
公开(公告)日：2023-11-15
申请号：EP21924111.4
申请日：2021-07-06
Applicant: Asymchem Laboratories (Tianjin) Co., Ltd.
Inventor： HONG, Hao , JAMES, Gage , XIAO, Yi , ZHANG, Na , JIAO, Xuecheng , MA, Yulei , MOU, Huiyan , WANG, Zujian , SUN, Kaihua , JIA, Ru , LIU, Fang , LIU, Wenjing
Abstract: Provided is a method for synthesizing a chiral amine compound. A transaminase is used to transaminate a ketone substrate under the action of an amino donor, to obtain the chiral amine compound; and the conserved amino acid sequence region of the transaminase at least includes a region 1 (MAGLWCVN) and a region 2 (YNTFFKT). With the transaminase with the specific conserved amino acid sequence region to synthesize a large sterically hindered chiral amine, the enzyme catalytic reaction volume is small, the synthesizing route is short, the product yield is high, a high-cost noble metal is not required for catalysis under the synthesizing conditions, three wastes are reduced, and the production cost is saved.
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