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公开(公告)号：EP4417690A1
公开(公告)日：2024-08-21
申请号：EP22947559.5
申请日：2022-07-29
发明人： HONG, Hao , JAMES, Gage , ZHANG, Na , JIAO, Xuecheng , MA, Yulei , MU, Huiyan , XUAN, Meijuan , ZHANG, Yanqing , ZHANG, Renkuan
摘要： The present disclosure provides a transaminase mutant and a method for preparing a chiral amine compound. Herein, the transaminase mutant includes: (a) a protein having an amino acid sequence as shown in SEQ ID NO: 1; or (b) a protein having an amino acid sequence as shown in SEQ ID NO: 2 or SEQ ID NO: 3; or (c) a protein that undergoes an amino acid mutation in at least one of the following sites of the amino acid sequence in (b): Y89, L380, N86, Y85, T91, P83, K90, S417, S424, F301, G164, T452 and the like, and has a transaminase function; or (d) a protein having more than 80% of the homology with the amino acid sequence defined in any one of (a), (b) or (c) and having a transaminase function.
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公开(公告)号：EP4261280A1
公开(公告)日：2023-10-18
申请号：EP21920431.0
申请日：2021-03-02
发明人： HONG, Hao , JAMES, Gage , XIAO, Yi , ZHANG, Na , JIAO, Xuecheng , LI, Rui , ZHANG, Yanqing , YANG, Yiming
摘要： Provided are an amino acid dehydrogenase mutant and use thereof. The amino acid sequence of the amino acid dehydrogenase mutant has a sequence as shown in SEQ ID NO: 1, and sites at which amino acid mutations occur include a K144G site. According to the amino acid dehydrogenase mutant, changes in protein structure and function are achieved, the enzyme activity is improved, and the usage amount of amino acid dehydrogenase is reduced. In addition, the enzyme specificity is also correspondingly improved, and the cost in industrial production of amino acids is reduced.
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