公开(公告)号：EP1364029A1

公开(公告)日：2003-11-26

申请号：EP02711847.0

申请日：2002-02-08

申请人： Aventis Pharma Deutschland GmbH

发明人： HABERMANN, Paul ,  ERTL, Johann

IPC分类号： C12N15/62 ,  C12N15/70 ,  C07K14/62 ,  C07K14/815 ,  C12N1/21 ,  C12P21/02 ,  C12P21/06

CPC分类号： C07K14/62 ,  C07K14/815 ,  C07K2319/035 ,  C07K2319/75 ,  C12N15/625 ,  C12N15/74

摘要： The invention relates to fusion proteins comprising a fusion part and a protein of interest, the combination of the two proteins leading to the fusion protein being secreted into the supernatant of a bacterial host and the protein of interest being present in its correct three-dimensional structure.

公开(公告)号：EP1222207A1

公开(公告)日：2002-07-17

申请号：EP00960664.1

申请日：2000-09-15

申请人： Aventis Pharma Deutschland GmbH

发明人： HABERMANN, Paul ,  ERTL, Johann ,  MEIWES, Johannes ,  SEIPKE, Gerhard

IPC分类号： C07K14/62 ,  C12N15/10 ,  C12N15/63 ,  C12N15/70

CPC分类号： C07K14/62 ,  C07K2319/00

摘要： The invention relates to a precursor of human insulin or an insulin analogue of the formula (I): Fus-B(1-30)-RDVP-Yn-A(1-21), whereby Fus is an optionally present fusion component of any sequence, B(1-30) is the B chain of human insulin, Y stands for an amino acid chain which ends with a C-terminal basic amino acid, n is 2 to 50 and indicates the length of the amino acid chain Y and A(1-21) is the A chain of human insulin. The A and/or the B chain can be modified by means of amino acid exchanges, deletions and/or additions. The invention also relates to a DNA coding therefor. The invention further relates to the production and the use of the precursor and the DNA as well as to a method for producing human insulin or an insulin analogue.