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    VERFAHREN ZUR ERHÖDELTAHUNG DES GEHALTS AN MEHRFACH UNGESÄTTIGTEN LANGKETTIGEN FETTSÄUREN IN TRANSGENEN ORGANISMEN
    1.
    发明公开
    VERFAHREN ZUR ERHÖDELTAHUNG DES GEHALTS AN MEHRFACH UNGESÄTTIGTEN LANGKETTIGEN FETTSÄUREN IN TRANSGENEN ORGANISMEN 有权
    方法用于增加转基因生物多不饱和长链脂肪酸的含量

    公开(公告)号：EP1769074A2

    公开(公告)日：2007-04-04

    申请号：EP05781182.0

    申请日：2005-07-15

    申请人： BASF Plant Science GmbH

    发明人： CIRPUS, Petra ,  BAUER, Jörg ,  QIU, Xiao ,  VRINTEN, Patricia

    IPC分类号： C12N15/09

    CPC分类号： C12N9/0006 ,  C12N9/20 ,  C12N9/88 ,  C12N15/8247

    摘要： The invention relates to a method for increasing the content of polyunsaturated long-chained fatty acids in an organism, by introducing nucleic acid coding for polypeptides and/or proteins having phopholipase, ketoacyl-CoA-reductase and/or dehydratase activity into the organisms. Advantageously, said enzymes stem from ostreococcus or thraustochytrium. The invention also relates to nucleic acid sequences, nucleic acid structures, vectors and organisms containing the inventive nucleic acid sequences, vectors containing the nucleic acid sequences and/or the nucleic acid structures, and transgenic organisms containing the above-mentioned nucleic acid sequences, nucleic acid structures and/or vectors. Advantageously, said above-mentioned nucleic acid frequencies, nucleic acid structures and vectors can optionally be expressed in the organism along with additional nucleic acid sequences coding for the polypeptides and/or proteins of fatty acids or lipid metabolism biosynthesis. Another advantage of the nucleic acid sequences of the fatty acids or lipid metabolism is that they are coded for Δ-9 elongase, Δ-8-desaturase, a Δ-5-desaturase, Δ-4-desaturase, Δ-12-desaturase, Δ-5-elongase and/or Δ-6-elongase activity. Said desaturases and elongases stem from organisms such as thalassiosira, euglena, isochrysis, physcomitrella, thraustochytrium, borago, phytophthora, crypthecodinium, oncorhynchus, primula, xenopus, ciona, arabidopsis, mortierella, caenorhabditis, phaeodactylum, ceratodon or ostreococcus. The invention further relates to oils, lipids, and/or fatty acids that are produced according the inventive method, and to the use thereof, in addition to the inventive unsaturated fatty acids and triglycerides having an increased content of unsaturated fatty acids and to the use thereof.

    PROMOTERS FROM BRASSICA NAPUS FOR SEED SPECIFIC GENE EXPRESSION
    4.
    发明公开
    PROMOTERS FROM BRASSICA NAPUS FOR SEED SPECIFIC GENE EXPRESSION 审中-公开
    油菜发起人种子特异性基因表达

    公开(公告)号：EP2222856A2

    公开(公告)日：2010-09-01

    申请号：EP08861278.3

    申请日：2008-12-15

    申请人： BASF Plant Science GmbH ,  Bioriginal Food & Science Corp.

    发明人： BAUER, Jörg ,  WETJEN, Tom ,  QIU, Xiao ,  WU, Guohai

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8234

    摘要： The present invention is concerned with means and methods for allowing tissue specific and, in particular, seed specific expression of genes. The present invention, accordingly, relates to a polynucleotide comprising an expression control sequence which allows seed specific expression of a nucleic acid of interest being operatively linked thereto. Moreover, the present invention contemplates vectors, host cells, non-human transgenic organisms comprising the aforementioned polynucleotide as well as methods and uses of such a polynucleotide.

    ELONGASEN UND VERFAHREN ZUR HERSTELLUNG MEHRFACH UNGESÄTTIGTER FETTSÄUREN IN TRANSGENEN ORGANISMEN
    5.
    发明公开
    ELONGASEN UND VERFAHREN ZUR HERSTELLUNG MEHRFACH UNGESÄTTIGTER FETTSÄUREN IN TRANSGENEN ORGANISMEN 有权
    延伸酶和过程对于在转基因生物生产多不饱和脂肪酸

    公开(公告)号：EP2176416A2

    公开(公告)日：2010-04-21

    申请号：EP08786637.2

    申请日：2008-07-30

    申请人： BASF Plant Science GmbH ,  Bioriginal Food & Science Corporation

    发明人： BAUER, Jörg ,  QIU, Xiao ,  VRINTEN, Patricia

    IPC分类号： C12N15/82 ,  C12N9/02

    CPC分类号： C12N9/1029 ,  C12N15/8247

    摘要： The present invention relates to polynucleotides from Pythium irregulare, Rhizopus oryzae and Euglena gracilis which code for elongases and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotides according to the invention, and to the polypeptides encoded by the polynucleotides. The invention furthermore relates to antibodies against the polypeptides according to the invention. Finally, the invention also relates to production processes for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions and to their use as drugs, cosmetics, foodstuffs, feedstuffs, preferably fish food, or food supplements.

    VERFAHREN ZUR ERHÖHUNG DES GEHALTS AN MEHRFACH UNGESÄTTIGTEN LANGKETTIGEN FETTSÄUREN IN TRANSGENEN ORGANISMEN
    6.
    发明授权
    VERFAHREN ZUR ERHÖHUNG DES GEHALTS AN MEHRFACH UNGESÄTTIGTEN LANGKETTIGEN FETTSÄUREN IN TRANSGENEN ORGANISMEN 有权
    方法用于增加转基因生物多不饱和长链脂肪酸的含量

    公开(公告)号：EP1769074B1

    公开(公告)日：2009-10-14

    申请号：EP05781182.0

    申请日：2005-07-15

    申请人： BASF Plant Science GmbH

    发明人： CIRPUS, Petra ,  BAUER, Jörg ,  QIU, Xiao ,  VRINTEN, Patricia

    IPC分类号： C12N15/82 ,  C12N15/55 ,  C12N9/20 ,  C12N15/53 ,  C12N9/04 ,  C12N15/60 ,  C12N9/88 ,  A01H5/00

    CPC分类号： C12N9/0006 ,  C12N9/20 ,  C12N9/88 ,  C12N15/8247

    摘要： The invention relates to a method for increasing the content of polyunsaturated long-chained fatty acids in an organism, by introducing nucleic acid coding for polypeptides and/or proteins having phopholipase, ketoacyl-CoA-reductase and/or dehydratase activity into the organisms. Advantageously, said enzymes stem from ostreococcus or thraustochytrium. The invention also relates to nucleic acid sequences, nucleic acid structures, vectors and organisms containing the inventive nucleic acid sequences, vectors containing the nucleic acid sequences and/or the nucleic acid structures, and transgenic organisms containing the above-mentioned nucleic acid sequences, nucleic acid structures and/or vectors. Advantageously, said above-mentioned nucleic acid frequencies, nucleic acid structures and vectors can optionally be expressed in the organism along with additional nucleic acid sequences coding for the polypeptides and/or proteins of fatty acids or lipid metabolism biosynthesis. Another advantage of the nucleic acid sequences of the fatty acids or lipid metabolism is that they are coded for Δ-9 elongase, Δ-8-desaturase, a Δ-5-desaturase, Δ-4-desaturase, Δ-12-desaturase, Δ-5-elongase and/or Δ-6-elongase activity. Said desaturases and elongases stem from organisms such as thalassiosira, euglena, isochrysis, physcomitrella, thraustochytrium, borago, phytophthora, crypthecodinium, oncorhynchus, primula, xenopus, ciona, arabidopsis, mortierella, caenorhabditis, phaeodactylum, ceratodon or ostreococcus. The invention further relates to oils, lipids, and/or fatty acids that are produced according the inventive method, and to the use thereof, in addition to the inventive unsaturated fatty acids and triglycerides having an increased content of unsaturated fatty acids and to the use thereof.

    VERFAHREN ZUR HERSTELLUNG MEHRFACH UNGESÄTTIGTER FETTSÄUREN IN TRANSGENEN
    10.
    发明公开
    VERFAHREN ZUR HERSTELLUNG MEHRFACH UNGESÄTTIGTER FETTSÄUREN IN TRANSGENEN 审中-公开
    VERFAHREN ZUR HERSTELLUNG MEHRFACHUNGESÄTTIGTERFETTSÄURENIN TRANSGENEN

    公开(公告)号：EP1654344A2

    公开(公告)日：2006-05-10

    申请号：EP04763291.4

    申请日：2004-07-16

    申请人： BASF Plant Science GmbH

    发明人： ZANK, Thorsten ,  BAUER, Jörg ,  CIRPUS, Petra ,  ABBADI, Amine ,  HEINZ, Ernst ,  QIU, Xiao ,  VRINTEN, Patricia ,  SPERLING, Petra ,  DOMERGUE, Frederic ,  MEYER, Astrid ,  KIRSCH, Jelena

    IPC分类号： C11B1/02

    CPC分类号： C12N15/8247 ,  A23D9/00 ,  A23D9/02 ,  A23L33/115 ,  A23V2002/00 ,  C11B1/00 ,  C12N9/0071 ,  C12N9/0083 ,  C12N9/0093 ,  C12N9/1029 ,  C12P7/6409 ,  C12P7/6427 ,  C12P7/6463 ,  C12P7/6472 ,  C12Y114/19 ,  C12Y114/19003 ,  C12Y203/01199 ,  Y02P20/52

    摘要： The invention relates to a method for the production of multiply-unsaturated fatty acids in an organism, into which nucleic acids have been introduced, which code for polypeptides with Δ-5 elongase activity. Said nucleic acid sequences, optionally with further nucleic acid sequences, coding for polypeptides for the biosynthesis of fatty acids and lipid metabolism, are advantageously expressed in the organism. Nucleic acid sequences coding for a Δ-6 desaturase, a Δ-5 desaturase, Δ-4 desaturase and/or Δ-6 elongase activity are particularly advantageous and, advantageously, said saturases and elongases are derived from Thalassiosira, Euglena or Ostreococcus. The invention further relates to a method for the production of oils and/or triacylglycerides with an increased content of long-chain, multiply-unsaturated fatty acids. A particular embodiment of the invention is a method for the production of unsaturated ω-3 fatty acids and a method for the production of triglycerides with an increased content of unsaturated fatty acids, in particular, of Δ-3 fatty acids with more than three double bonds. Also disclosed is the production of a transgenic organism, preferably a transgenic plant, or a transgenic microorganism with increased content of ω-3 fatty acids, oils or lipids with ω-3 double bonds as a result of the expression of the elongases and desaturases employed in the above method, preferably in combination with ω-3 desaturases, for example a ω-3 desaturase from fungi of the family Pythiaceae such as the genus Phytophtora, for example, the genus and species Phytophtora infestans, or a ω-3 desaturase from algae such as the family Prasinophyceae, for example, the genus Ostreococcus and, particularly, the genus and species Ostreococcus tauri or diatomaceæ such as the genus Thalassiosira and, particularly, the genus and species Thalassiosira pseudonana. The invention also relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms containing the nucleic acid sequences and/or the nucleic acid constructs and transgenic organisms containing said nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced according to the above method and use thereof and, furthermore, unsaturated fatty acids and triglycerides with an increased content of unsaturated fatty acids and use thereof.

    摘要翻译： 生产多不饱和脂肪酸（I）包括将编码δ9延伸酶或δ6去饱和酶，δ8去饱和酶或δ6延伸酶，δ5去饱和酶，δ5延伸酶和δ4去饱和酶活性的核酸序列引入生物体中以产生转基因生物 其总脂质包括至少1重量％（I）。 制备式（I）的多不饱和脂肪酸包括将编码Δ9延伸酶或δ6去饱和酶，δ8去饱和酶或δ6延伸酶，δ5去饱和酶，δ5延伸酶和δ4去饱和酶活性的核酸序列引入生物体中以产生 其总脂质包含至少1重量％的式（I）的转基因生物。 溶血磷脂酰乙醇胺，溶血磷脂酰甘油，溶血磷脂酰丝氨酸，溶血磷脂酰肌醇，鞘磷脂或OCH 2CH（OR 3））CH 2 OR 2>;（3）其中R 1> OH，辅酶A（硫酯），溶血磷脂酰胆碱，溶血磷脂酰乙醇胺， R 2> H，溶血磷脂酰胆碱，溶血磷脂酰乙醇胺，溶血磷脂酰甘油，溶血磷脂酰丝氨酸，溶血磷脂酰肌醇，（未饱和的）2-24C烷基羰基或CO（CH 2）n（CH = CHCH 2）m（CH 2）pCH 3; R 3> H，（未）饱和的2-24C烷基羰基或CO（CH 2）n（CH = CHCH 2）m（CH 2）pCH 3; n：2-9; m：2-6; p：0或3.还包括：（1）上述生产的油，脂或脂肪酸的独立权利要求。 （2）包含如上生产并衍生自转基因植物的多不饱和脂肪酸的油，脂质或脂肪酸组合物; （3）编码具有本说明书中给出的6个氨基酸序列之一的δ5延伸酶多肽的分离的核酸序列; （4）分离的核酸序列，其编码δ6延伸酶多肽并具有说明书中给出的4个核苷酸序列之一; （5）编码ω3去饱和酶多肽的分离的核酸序列，并具有说明书中给出的2个核苷酸序列之一; （6）分离的核酸序列，其编码δ6去饱和酶多肽并具有说明书中给出的2个核苷酸序列之一; （7）分离的核酸序列，其编码δ5去饱和酶多肽并具有说明书中给出的4个核苷酸序列之一; （8）编码Δ4去饱和酶多肽的分离的核酸序列，并且具有说明书中给出的2个核苷酸序列之一; （9）分离的核酸序列，其编码δ12去饱和酶多肽并具有说明书中给出的2个核苷酸序列之一; （10）由上述分离的核酸序列编码的氨基酸序列; （11）包含如上所述的分离的核酸与一种或多种调节信号连接的遗传构建体; （12）载体，其含有如上所述的核酸或构建体; （13）含有如上所述的核酸，构建体或载体的转基因非人生物体。