公开(公告)号：EP0965045B1

公开(公告)日：2002-01-09

申请号：EP98905473.9

申请日：1998-01-30

申请人： BIO MERIEUX

发明人： PERRIN, Agnès ,  THERETZ, Alain ,  MANDRAND, Bernard

IPC分类号： G01N33/553 ,  G01N33/548 ,  G01N33/543

CPC分类号： G01N33/54326 ,  B01J2219/00648 ,  B01J2219/00659 ,  G01N33/54306 ,  Y10S436/801 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/807 ,  Y10S436/824 ,  Y10T436/24

摘要： The invention concerns a method for isolating at least an analyte (4) in a medium, in which it is distributed, consisting in: (a) providing at least one reagent (3) comprising a free support, in discrete form, exhibiting at least a receptor of the analyte; (b) providing another capture support, the accessible surface of which comprises at least an effective or active zone, of limited surface, exhibiting at least another receptor (10) of an entity to be captured, viz. of said analyte or said receptor; (c) contacting the reagent (3) with a liquid sample obtained from the medium containing the analyte, for forming an intermediate medium comprising, also in discrete form and distributed in said medium, a complex between said support, the receptor and the analyte; (d) contacting the accessible surface of said other support with at least a capture reactant, viz. the reagent that has not reacted during step (c) or the complex, for binding said capture reactant to said accessible surface, and obtaining a concentrated deposit in said effective zone, fixed on said other support by the bond of said other receptor with said receptor or analyte; (e) concentrating the deposit of said capture reactant in said effective zone, by increasing the amount of said reactant per surface unit in said effective zone.

公开(公告)号：EP1381862A2

公开(公告)日：2004-01-21

申请号：EP02726283.1

申请日：2002-04-25

申请人： BIO MERIEUX

发明人： PERRIN, Agnès ,  THERETZ, Alain ,  DELAIR, Thierry ,  MANDRAND, Bernard

IPC分类号： G01N33/543 ,  C12Q1/68

CPC分类号： B82Y30/00 ,  B01J2219/00387 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00655 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/0072 ,  B01L3/0241 ,  B01L3/50857 ,  B01L2400/021 ,  B01L2400/043 ,  C40B60/14 ,  G01N33/54333

摘要： The invention concerns a method for depositing on a substrate (1) a product of interest (2), in a small elementary surface (3), called spot, which consists in: a) preparing or providing a liquid medium (4) comprising a carrier and the product of interest (2); (b) from the liquid medium, forming a drop (5), and setting said drop on the surface (1a) of the substrate, in contact with the liquid medium; (c) eliminating the carrier of the drop (5), for example by drying, so as to allow said spot to remain. The invention is characterised in that it consists, in combination, in: (1) preparing or providing a magnetic support (6), distributed in the carrier; (2) binding the product of interest (2) to the magnetic support (6), so that the liquid medium, wherefrom the drop (5) is formed, comprises transport particles (7); (3) at least while eliminating the carrier of the drop (5), generating a magnetic field (8) passing through the surface (1a) of the substrate (1), so that the resulting spot (3) comprises the transport particles, and hence the product of interest.

公开(公告)号：EP0965045A1

公开(公告)日：1999-12-22

申请号：EP98905473.0

申请日：1998-01-30

申请人： BIO MERIEUX

发明人： PERRIN, Agnès ,  THERETZ, Alain ,  MANDRAND, Bernard

IPC分类号： G01N33

CPC分类号： G01N33/54326 ,  B01J2219/00648 ,  B01J2219/00659 ,  G01N33/54306 ,  Y10S436/801 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/807 ,  Y10S436/824 ,  Y10T436/24

摘要： The invention concerns a method for isolating at least an analyte (4) in a medium, in which it is distributed, consisting in: (a) providing at least one reagent (3) comprising a free support, in discrete form, exhibiting at least a receptor of the analyte; (b) providing another capture support, the accessible surface of which comprises at least an effective or active zone, of limited surface, exhibiting at least another receptor (10) of an entity to be captured, viz. of said analyte or said receptor; (c) contacting the reagent (3) with a liquid sample obtained from the medium containing the analyte, for forming an intermediate medium comprising, also in discrete form and distributed in said medium, a complex between said support, the receptor and the analyte; (d) contacting the accessible surface of said other support with at least a capture reactant, viz. the reagent that has not reacted during step (c) or the complex, for binding said capture reactant to said accessible surface, and obtaining a concentrated deposit in said effective zone, fixed on said other support by the bond of said other receptor with said receptor or analyte; (e) concentrating the deposit of said capture reactant in said effective zone, by increasing the amount of said reactant per surface unit in said effective zone.